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Showing: 2251 - 2300 of 27839 results

  1. pOliSpec3'

    Plasmid
    #228550
    Purpose
    Template for PCR amplification for gRNA cloning. The PCR product recombines in vivo with a PCRs products from pOliSpec5' and oligo-spacer amplifications, to clone gRNA plasmid.
    Depositor
    Jeferson Gross
    Insert
    Truncated 3' region of aadA
    Use
    CRISPR
    Available Since
    Dec. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    228550-plasmid-map-sequence-id-454859

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  3. pAAV-FLEX-EGFPL10a (AAV5)

    Viral Prep
    #98747-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-FLEX-EGFPL10a (#98747). In addition to the viral particles, you will also receive purified pAAV-FLEX-EGFPL10a plasmid DNA. Cre-dependent EGFPL10a (EGFP-tagged ribosomal protein) expression for translating ribosome affinity purification (TRAP). These AAV preparations are suitable purity for injection into animals.
    Depositor
    Nathaniel Heintz, Alexander Nectow, Eric Schmidt
    Article
    Nectow et al Cell Rep. 2017 Apr 18;19(3):655-667. doi
    Promoter
    EF1a
    Tags
    EGFP (Cre-dependent)
    Available Since
    Sept. 28, 2018
    Availability
    Academic Institutions and Nonprofits only

  4. Sc RFC[hk-1s + 5]-pLANT/RIL

    Plasmid
    #239200
    Purpose
    Overeexpress Sc RFC1 (truncated N-terminus; N-terminal his tag, PKA kinase motif) in E. coli
    Depositor
    Michael O'Donnell
    Inserts
    RFC1 (RFC1 Budding Yeast)
    RFC5 (RFC5 Budding Yeast)
    Tags
    His-10 + PKA kinase motif on truncated Sc RFC1
    Expression
    Bacterial
    Mutation
    N-terminal truncation
    Available Since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    239200-plasmid-map-sequence-id-476583

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  7. pLSU1/t35s[]:mCherry-I{PIV-180}:NosT

    Plasmid
    #212179
    Purpose
    This binary vector expresses mCherry containing the PIV intron (180bp into the gene) with the truncated 35sCAMV promoter
    Depositor
    Wayne Curtis
    Insert
    mCherry-I{PIV-180}
    Expression
    Plant
    Mutation
    Truncated mCherry, missing the first 9 amino acid…
    Promoter
    truncated 35sCaMV
    Available Since
    Aug. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212179-plasmid-map-sequence-id-443888

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  8. pOpen-RNAse A

    Plasmid
    #165565
    Purpose
    Ribonuclease A or RNase A; endoribonuclease purified from bovine pancreas. Important enzyme for the removal of RNA for RNA free DNA purification reactions such as plasmid DNA purification and genomic DNA purification, RNA removal from recombinant protein preparations, ribonuclease protection assays, mapping single-base mutations in DNA/RNA.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    RNAse A
    Use
    Synthetic Biology
    Available Since
    Aug. 17, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165565-plasmid-map-sequence-id-340093

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  10. pOCC102-His-MBP-DDX3X(WT)

    Plasmid
    #219988
    Purpose
    DDX3X(WT) protein purification
    Depositor
    Simon Alberti
    Insert
    DDX3X(WT) (DDX3X Human)
    Tags
    6xHis and MBP
    Expression
    Insect
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219988-plasmid-map-sequence-id-436790

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  16. pOCC120-MBP-DDX3X-monoGFP-His

    Plasmid
    #219985
    Purpose
    DDX3X(WT) protein purification
    Depositor
    Simon Alberti
    Insert
    DDX3X(WT) (DDX3X Human)
    Tags
    MBP and monoGFP and 6xHis
    Expression
    Insect
    Available Since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    219985-plasmid-map-sequence-id-436890

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  18. pTXB1-sfGFP

    Plasmid
    #51562
    Purpose
    Expression of superfolder GFP for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone)
    Depositor
    Sankar Adhya, Francisco Malagon
    Insert
    superfolder GFP
    Tags
    Intein-Chiting Binding Domanin
    Expression
    Bacterial
    Available Since
    March 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    51562-plasmid-map-sequence-id-78800

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  22. pRAV12

    Plasmid
    #67713
    Purpose
    Parental plasmid for transcription of RNAs with a HdV/M-domain 3'-tag for native purification
    Depositor
    Robert Batey, Jeffrey Kieft
    Article
    Kieft et al RNA. 2004 Jun;10(6):988-95.
    Insert
    Transcription cassette for 3'-tagged RNAs
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    T7 RNA polymerase
    Available Since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    67713-plasmid-map-sequence-id-126588

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  24. pLSU1/t35s[TMV]:mNeonGreen-I{PDK-198}:NosT

    Plasmid
    #212170
    Purpose
    This binary vector expresses mNeonGreen containing the castor bean catalase (PDK) intron (198bp into the gene) with the truncated 35sCAMV promoter and the tomato mosaic virus UTR
    Depositor
    Wayne Curtis
    Insert
    Tomato codon optimized version of mNeonGreen-I{PDK-198}
    Expression
    Plant
    Mutation
    mNeonGreen codon-optimized for tomato and PDK int…
    Promoter
    truncated 35sCaMV
    Available Since
    March 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    212170-plasmid-map-sequence-id-422699

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  27. RHCglo

    Plasmid
    #80169
    Purpose
    Minigene reporter for identification and analysis of cis elements and trans factors affecting pre-mRNA splicing
    Depositor
    Thomas Cooper
    Article
    Singh et al Biotechniques. 2006 Aug;41(2):177-81.
    Insert
    segments of chicken cardiac troponin T (Tnnt2) introns 4 and 5 flanking an artifical exon
    Expression
    Mammalian
    Available Since
    Sept. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    80169-plasmid-map-sequence-id-150808

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  33. pYCTK034

    Plasmid
    #176738
    Purpose
    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Bar1 gene of Saccharomyces cerevisiae (barrier part).
    Depositor
    Victor Sourjik
    Article
    Krink et al bioRxiv 2021
    Insert
    bar1Sc
    Use
    Synthetic Biology
    Available Since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    176738-plasmid-map-sequence-id-351575

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  34. pYCTK027

    Plasmid
    #176731
    Purpose
    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Saccharomyces cerevisiae (receiver part).
    Depositor
    Victor Sourjik
    Article
    Krink et al bioRxiv 2021
    Insert
    ste2Sc
    Use
    Synthetic Biology
    Available Since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    176731-plasmid-map-sequence-id-351583

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  35. pYCTK002

    Plasmid
    #176706
    Purpose
    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor-responsive promoter FUS1 of S. cerevisiae (response part)
    Depositor
    Victor Sourjik
    Article
    Krink et al bioRxiv 2021
    Insert
    P-FUS1
    Use
    Synthetic Biology
    Available Since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    176706-plasmid-map-sequence-id-351547

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  37. pTXB1-FIS

    Plasmid
    #51564
    Purpose
    Expression of E. coli FIS for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone
    Depositor
    Sankar Adhya, Francisco Malagon
    Insert
    FIS
    Tags
    Intein-Chiting Binding Domanin
    Expression
    Bacterial
    Available Since
    March 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    51564-plasmid-map-sequence-id-78815

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  39. pTXB1-CI

    Plasmid
    #51565
    Purpose
    Expression of bacteriophage lambda CI for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone
    Depositor
    Sankar Adhya, Francisco Malagon
    Insert
    cI
    Tags
    Intein-Chiting Binding Domanin
    Expression
    Bacterial
    Available Since
    March 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    51565-plasmid-map-sequence-id-78820

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  41. pAIP4_4IPC

    Plasmid
    #234229
    Purpose
    Heterologous protein expression of replication protein A 70 kDa DNA-binding subunit in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4IPC (RPA1 Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234229-plasmid-map-sequence-id-464194

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  44. pYCTK006

    Plasmid
    #176710
    Purpose
    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor-responsive promoter SST2 (response part).
    Depositor
    Victor Sourjik
    Article
    Krink et al bioRxiv 2021
    Insert
    P-SST2
    Use
    Synthetic Biology
    Available Since
    April 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    176710-plasmid-map-sequence-id-351557

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  45. pYCTK001

    Plasmid
    #176705
    Purpose
    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor-responsive promoter DIG2 of S. cerevisiae (response part)
    Depositor
    Victor Sourjik
    Article
    Krink et al bioRxiv 2021
    Insert
    P-DIG2
    Use
    Synthetic Biology
    Available Since
    Feb. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    176705-plasmid-map-sequence-id-351600

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  46. pYCTK007

    Plasmid
    #176711
    Purpose
    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor-responsive promoter YPS1 (response part).
    Depositor
    Victor Sourjik
    Article
    Krink et al bioRxiv 2021
    Insert
    P-YPS1
    Use
    Synthetic Biology
    Available Since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    176711-plasmid-map-sequence-id-351599

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  47. pYCTK016

    Plasmid
    #176720
    Purpose
    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor gene of Saccharomyces cerevisiae (sender part).
    Depositor
    Victor Sourjik
    Article
    Krink et al bioRxiv 2021
    Insert
    mfalpha1Sc
    Use
    Synthetic Biology
    Available Since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    176720-plasmid-map-sequence-id-351593

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  48. pYCTK005

    Plasmid
    #176709
    Purpose
    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor-responsive promoter PRM1 of S. cerevisiae (response part)
    Depositor
    Victor Sourjik
    Article
    Krink et al bioRxiv 2021
    Insert
    P-PRM1
    Use
    Synthetic Biology
    Available Since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    176709-plasmid-map-sequence-id-351556

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  49. pYCTK004

    Plasmid
    #176708
    Purpose
    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor-responsive promoter MSG5 of S. cerevisiae (response part)
    Depositor
    Victor Sourjik
    Article
    Krink et al bioRxiv 2021
    Insert
    P-MSG5
    Use
    Synthetic Biology
    Available Since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    176708-plasmid-map-sequence-id-351555

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  50. pYCTK003

    Plasmid
    #176707
    Purpose
    The plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the alpha-factor-responsive promoter FUS3 (response part).
    Depositor
    Victor Sourjik
    Article
    Krink et al bioRxiv 2021
    Insert
    P-FUS3
    Use
    Synthetic Biology
    Available Since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    176707-plasmid-map-sequence-id-351554

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Showing: 2251 - 2300 of 27839 results