We narrowed to 2,788 results for: DAP

1. pRK-flag-USP19 494-1290

   Plasmid

   #78580

   Purpose

   Expression flag-USP19 494-1290 in mammalian cells

   Depositor

   Yihong Ye

   Article

   Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

   Insert

   flag-USP19 494-1290 (USP19 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   deleted amino acid 1-493 and mutated 1291 residue…

   Promoter

   CMV

   Available Since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pET28a-Hook3 aa 1-160

   Plasmid

   #74607

   Purpose

   Expresses Hook domain of Hook3 in bacteria for purification

   Depositor

   Ron Vale

   Article

   Schroeder et al J Cell Biol. 2016 Aug 1;214(3):309-18. d

   Insert

   human Hook3 amino acids 1-160 (HOOK3 Human)
   

   Use

   Tags

   6x His, Strep II, and superfolder GFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. M34 Xdsh PDZ bait-pGBT9

   Plasmid

   #17190

   Depositor

   Randall Moon

   Article

   Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

   Insert

   Xdsh (dvl2.L Frog)
   

   Use

   Tags

   Gal4-DBD

   Expression

   Yeast

   Mutation

   Dsh bp 343-1191

   Promoter

   Available Since

   Feb. 1, 2008

   Availability

   Academic Institutions and Nonprofits only

4. XE160 XDshHA-pt mutant PDZ-CS2+

   Plasmid

   #16780

   Depositor

   Randall Moon

   Article

   Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

   Insert

   Dishevelled (dvl2.L Frog)
   

   Use

   Xenopus expression

   Tags

   HA

   Expression

   Mutation

   point mutation in the binding pocket of the PDZ b…

   Promoter

   Available Since

   March 24, 2008

   Availability

   Academic Institutions and Nonprofits only

5. XE161 XDshHA-triple pt mutant-CS2+

   Plasmid

   #16779

   Depositor

   Randall Moon

   Article

   Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

   Insert

   Dishevelled (dvl2.L Frog)
   

   Use

   Xenopus expression

   Tags

   HA

   Expression

   Mutation

   triple point mutation outside of the PDZ binding …

   Promoter

   Available Since

   March 14, 2008

   Availability

   Academic Institutions and Nonprofits only

6. EC.SurA.pTYB1.a21-281&390-428.L239R

   Plasmid

   #15959

   Depositor

   David McKay

   Article

   Xu et al J Mol Biol. 2007 Oct 19. 373(2):367-81.

   Insert

   Escherichia coli surA delta PPIase domain 2
   

   Use

   Tags

   intein

   Expression

   Bacterial

   Mutation

   residues 282-389 deleted; L239R mutation

   Promoter

   Available Since

   Nov. 9, 2007

   Availability

   Academic Institutions and Nonprofits only

7. Danio rerio cryaa V62T

   Plasmid

   #47630

   Purpose

   Recombinant protein production

   Depositor

   Mason Posner

   Article

   Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

   Insert

   Alpha A-crystallin (cryaa Zebrafish)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Changed Valine 62 to Threonine

   Promoter

   T7

   Available Since

   Oct. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. Danio rerio cryaa T147V

   Plasmid

   #47628

   Purpose

   Recombinant protein production

   Depositor

   Mason Posner

   Article

   Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

   Insert

   Alpha A-crystallin (cryaa Zebrafish)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Changed Theronine 147 to Valine

   Promoter

   T7

   Available Since

   Sept. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. Danio rerio cryaa C143S

   Plasmid

   #47619

   Purpose

   Recombinant protein production

   Depositor

   Mason Posner

   Article

   Posner et al PLoS One. 2012;7(3):e34438. doi: 10.1371

   Insert

   Alpha A-crystallin (cryaa Zebrafish)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Cysteine 143 to Serine

   Promoter

   T7

   Available Since

   Sept. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. EC.SurA.pTYB1.a21-281&390-428.M231R

    Plasmid

    #15958

    Depositor

    David McKay

    Article

    Xu et al J Mol Biol. 2007 Oct 19. 373(2):367-81.

    Insert

    Escherichia coli surA delta PPIase domain 2
    

    Use

    Tags

    intein

    Expression

    Bacterial

    Mutation

    residues 282-389 deleted; M231R mutation

    Promoter

    Available Since

    Nov. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

11. pRK5-FLAG-p14

    Plasmid

    #42330

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

    Insert

    p14 (LAMTOR2 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available Since

    March 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-FLAG-FADD

    Plasmid

    #78802

    Purpose

    To overexpress FADD in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Lee et al Nat Commun. 2012;3:978. doi: 10.1038/nco

    Insert

    FADD (FADD Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3 myc card9

    Plasmid

    #16253

    Depositor

    William Kaelin

    Article

    Yang et al Mol Cell. 2007 Oct 12. 28(1):15-27.

    Insert

    card9 (CARD9 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. psigma1-EGFP

    Plasmid

    #53611

    Purpose

    Expresses sigma1 of adaptor protein of AP1

    Depositor

    Tom Kirchhausen

    Article

    Kural et al Cell Rep. 2012 Nov 29;2(5):1111-9. doi:

    Insert

    sigma 1 (Ap1s1 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-AMPKsub-YPet-NES

    Plasmid

    #84635

    Purpose

    Encodes C-terminal (substrate) fragment of bimolecular AMPK/BRSK activity reporter (bimABKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NES

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    AMPKsub-YPet-NES
    

    Use

    Tags

    6xHis, Nuclear export signal (NES), T7 tag (gene …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRS315e_pGal-dCas9-SH3-pGal-PmCDA1-SHL

    Plasmid

    #79614

    Purpose

    Expresses dCas9-SH3 and PmCDA1-SHL in yeast cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Inserts

    SpCas9
    PmCDA1
    

    Use

    Tags

    SH3 domain and SHL

    Expression

    Yeast

    Mutation

    D10A and H840A for nuclease deficient Cas9

    Promoter

    pGal1 and pGal10

    Available Since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-mCherry-LKB1(KD)

    Plasmid

    #84640

    Purpose

    mCherry-tagged, LKB1(K78I); kinase-dead mutant

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    mCherry-LBK1(K78I) (STK11 Human, Synthetic)
    

    Use

    Tags

    6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …

    Expression

    Mammalian

    Mutation

    Lysine 78 mutated to Isoleucine; kinase dead

    Promoter

    CMV

    Available Since

    May 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. Intersectin I SH3 A domain (human)

    Plasmid

    #47413

    Depositor

    Peter McPherson

    Article

    Yamabhai et al J Biol Chem. 1998 Nov 20;273(47):31401-7

    Insert

    Intersectin SH3 domain A (ITSN1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    only SH3 domain A

    Promoter

    tac

    Available Since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3.1_pCMV-nCas-PmCDA1 pH1-gRNA(HPRT)

    Plasmid

    #79619

    Purpose

    Expresses nCas9-PmCDA1 and gRNA(HPRT) in mammalian cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    3xFlag-PmCDA1

    Expression

    Mammalian

    Mutation

    D10A for nickase Cas9

    Promoter

    pCMV

    Available Since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1_pCMV-dCas-PmCDA1 pH1-gRNA(HPRT)

    Plasmid

    #79621

    Purpose

    Expresses dCas9-PmCDA1 and gRNA(HPRT) in mammalian cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    PmCDA1

    Expression

    Mammalian

    Mutation

    D10A and H840A for nuclease deficient Cas9

    Promoter

    pCMV

    Available Since

    Sept. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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