We narrowed to 7,288 results for: ef1a

1. pAAV-EF1a-mCherry-IRES-Flpo

   Plasmid

   #55634

   Purpose

   Expresses Flpo in Mammalian Cells

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Has Service

   AAV Retrograde and AAV1

   Inserts

   mCherry
   Flpo
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a and Ef1a/IRES

   Available since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. CoV2-Spike-EF1a-D614G-N501Y

   Plasmid

   #177939

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Spike proteins (D614G, N501Y). This version gives lower expression of Spike and is more convenient for generating VLPs.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Spike protein (D614G, N501Y) (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D614G, N501Y

   Promoter

   Available since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLX304 Flag-mCherry-eEF1A1_IRES-GFP

   Plasmid

   #198383

   Purpose

   eEF1A1 fluorescent reporter

   Depositor

   Jack Taunton

   Article

   Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

   Insert

   eEF1A1 (EEF1A1 Human)
   

   Use

   Lentiviral

   Tags

   Flag-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. lenti-EF1a-dCas9-VP64-Puro

   Plasmid

   #99371

   Purpose

   3rd generation lenti vector encoding dCas9-VP64 with 2A puromycin resistance marker (EF1a-dCas9-VP64-T2A-Puro-WPRE)

   Depositor

   Kristen Brennand

   Article

   Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

   Insert

   dCas9-VP64-T2A-Puro
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Aug. 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRRL sEF1a HA.NLS.Sce(opt).T2A.IFP

   Plasmid

   #31484

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

   Insert

   EF1s HA NLS Sce(opt) T2A IFP1.4
   

   Use

   Lentiviral

   Tags

   HA and NLS

   Expression

   Mutation

   "Opt" indicates the I-SceI coding seque…

   Promoter

   Available since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-EF1A-CD19.8h.8TM.28.3z

   Plasmid

   #200670

   Purpose

   Modular CD8hinge-CD8transmembrane-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

   Depositor

   Michele Bernasconi

   Article

   Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):

   Insert

   FMC63-CD8HD-CD8TM-CD28-CD3z-P2A-EGFP (CD8A, CD28, CD247 Human)
   

   Use

   Lentiviral

   Tags

   MycTag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a-short

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSIN4-EF1a-ETV2-IRES-Puro

   Plasmid

   #61061

   Purpose

   lentiviral vector for ETV2

   Depositor

   Igor Slukvin

   Article

   Elcheva et al Nat Commun. 2014 Jul 14;5:4372. doi: 10.

   Insert

   ETV2 (ETV2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1alpha

   Available since

   March 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSB-TRE-CRISPRoff-EF1A-TetOn

   Plasmid

   #203355

   Purpose

   Drug-inducible expression of CRISPRoff components needed for DNA methylation and gene repression with Sleeping Beauty backbone

   Depositor

   Jonathan Brunger

   Article

   Gil et al Bio Protoc. 2024 Jan 5;14(1):e4913. doi:

   Insert

   CRISPRoff-TetOn
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX-EF1a-CCO-IRES-Puromycin

   Plasmid

   #134861

   Purpose

   Expresses EGFP-tagged inner mitochondrial membrane marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   cytochrome c oxidase subunit VIII (COX8A Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-EF1a-DIO-JEDI-2P-Kv-WPRE

    Plasmid

    #179459

    Purpose

    Double floxed soma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-2P in AAV production vector expressed under the mammalian promoter (EF1a)

    Depositor

    Francois St-Pierre

    Article

    Liu et al Cell. 2022 Aug 16. pii: S0092-8674(22)00

    Has Service

    AAV1

    Insert

    JEDI-2P-Kv
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAF0689 Ef1a-Pa01_attB-BFP acceptor

    Plasmid

    #193472

    Purpose

    Acceptor plasmid for 3 plasmid recombination assay

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Pa01_attB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-EF1a-DIO-GRAB_g5-HT3.0

    Plasmid

    #208726

    Purpose

    Expresses the green 5-HT sensor GRAB_g5-HT3.0 in neurons in the presence of Cre recombinase

    Depositor

    Yulong Li

    Article

    Deng et al bioRxiv 2023.05.27.542566

    Insert

    Green fluorescent 5-HT sensor GRAB_g5-HT3.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEF1a-Ronin-FLAG-IRES-Neo

    Plasmid

    #28020

    Depositor

    Thomas Zwaka

    Article

    Dejosez et al Genes Dev. 2010 Jul 15. 24(14):1479-84.

    Insert

    Ronin (THAP11 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-Ef1a-hMGT-PGK-puro

    Plasmid

    #172394

    Purpose

    Lentiviral expression vector for human direct cardiac reprogramming

    Depositor

    Li Qian

    Article

    Garbutt et al STAR Protoc. 2020 Jun 19;1(1). doi: 10.1

    Insert

    Mef2c, Gata4, Tbx5 (MEF2C, GATA4, TBX5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-Ef1a-DIO-oG-WPRE-hGH

    Plasmid

    #74290

    Purpose

    pAAV vector to express oG (optimized Glycoprotein) in a Cre-dependent manner

    Depositor

    Edward Callaway

    Article

    Kim et al Cell Rep. 2016 Apr 26;15(4):692-699. doi

    Insert

    oG (optimized Glycoprotein)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    chimeric glycoprotein

    Promoter

    Ef1a

    Available since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHLS-EF1a-FKBP12-Gag(HIV)

    Plasmid

    #138476

    Purpose

    Expresses FKBP12 fused Gag for packaging FRB-SpCas9 into NanoMEDIC particle.

    Depositor

    Akitsu Hotta

    Article

    Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

    Insert

    HIV Gag
    

    Use

    Tags

    FRBP12 and Lynn Myristolation site

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLVH-EF1a-GFP-P2A-OCT4

    Plasmid

    #130692

    Purpose

    Lentiviral plasmids encoding human OCT4 (POU5f1) along with GFP linked via P2A.

    Depositor

    Kejin Hu

    Article

    Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

    Insert

    human OCT4 (POU5F1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVH-EF1a-GFP-P2A-MYC

    Plasmid

    #130695

    Purpose

    Lentiviral plasmids encoding human MYC with co-expressoin of GFP mediated by P2A.

    Depositor

    Kejin Hu

    Article

    Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

    Insert

    human MYC (MYC Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC CBA-SpCas9.EF1a-BFP.sgLMNA

    Plasmid

    #98971

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9 and a sgRNA targeting LMNA. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNA and spCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. Lenti-UbC-LARGE1-EF1a-BSD

    Plasmid

    #205151

    Purpose

    Express UbC-driven LARGE1 and EF-1a driven BSD

    Depositor

    Monkol Lek

    Insert

    LARGE xylosyl- and glucuronyltransferase 1 (LARGE1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UbC

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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