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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 947 results for: multisite gateway

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Showing: 901 - 920 of 947 results

  2. pEF5/FRT/DEST-3xHA/mGli3/Flag P1-6A

    Plasmid
    #51248
    Purpose
    Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907,980,1006 mutated to Ala
    Depositor
    Rajat Rohatgi
    Publication
    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gli3 (Gli3 Mouse)
    Use
    Flpin system
    Tags
    3xHA-TEV and Flag
    Expression
    Mammalian
    Mutation
    amino acids 849,865,877,907,980,1006 mutated to A…
    Promoter
    EF1a
    Available since
    March 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  3. pEF5/FRT/DEST-3xHA/mGli2 P1-6A

    Plasmid
    #51251
    Purpose
    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 789,805,817,848,923,956 mutated to Ala
    Depositor
    Rajat Rohatgi
    Publication
    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gli2 (Gli2 Mouse)
    Use
    Flpin system
    Tags
    3xHA-TEV
    Expression
    Mammalian
    Mutation
    amino acids 789,805,817,848,923,956 mutated to Ala
    Promoter
    EF1a
    Available since
    Feb. 25, 2014
    Availability
    Academic Institutions and Nonprofits only

  4. pEF5/FRT/DEST-3xHA/mGli2 Pc-gE

    Plasmid
    #51256
    Purpose
    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248 mutated to Glu
    Depositor
    Rajat Rohatgi
    Publication
    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gli2 (Gli2 Mouse)
    Use
    Flpin system
    Tags
    3xHA-TEV
    Expression
    Mammalian
    Mutation
    amino acids 216,220,224,230,248 mutated to Glu
    Promoter
    EF1a
    Available since
    Feb. 25, 2014
    Availability
    Academic Institutions and Nonprofits only

  5. pEF5/FRT/DEST-3xHA/mGli2 Pc-gA/P1-6A

    Plasmid
    #51257
    Purpose
    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 216,220,224,230,248,789,805,817,848,923,956 mutated to Ala
    Depositor
    Rajat Rohatgi
    Publication
    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gli2 (Gli2 Mouse)
    Use
    Flpin system
    Tags
    3xHA-TEV
    Expression
    Mammalian
    Mutation
    amino acids 216,220,224,230,248,789,805,817,848,9…
    Promoter
    EF1a
    Available since
    Feb. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    51257-plasmid-map-sequence-id-501235

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  7. p3E-mTagBFP

    Plasmid
    #75175
    Purpose
    TagBFP (blue) fluorophore with stop codon for C-terminal fusions
    Depositor
    Nicholas Cole
    Publication
    Don et al Zebrafish. 2016 Sep 15.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TagBFP
    Use
    Three prime entry vector for multisite gateway th…
    Promoter
    n/a
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    75175-plasmid-map-sequence-id-154499

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  8. pME-H2B-mCerulean3-P2A-mKOFP2-CAAX

    Plasmid
    #75154
    Purpose
    Nuclear mCerulean2 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with 5’ promoters.
    Depositor
    Nicholas Cole
    Publication
    Don et al Zebrafish. 2016 Sep 15.
    Insert
    H2B-mCerulean3-P2A-mKOFP2-CAAX
    Use
    Middle entry vector for multisite gateway three f…
    Promoter
    n/a
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    75154-plasmid-map-sequence-id-142108

    Enlarge

  11. p3E-mTagBFP-CAAX

    Plasmid
    #75176
    Purpose
    Membrane targeted TagBFP (blue) fluorophore with stop codon for C-terminal fusions
    Depositor
    Nicholas Cole
    Publication
    Don et al Zebrafish. 2016 Sep 15.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mTagBFP-CAAX
    Use
    Three prime entry vector for multisite gateway th…
    Promoter
    n/a
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    75176-plasmid-map-sequence-id-154502

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  12. pME-mVenus-CAAX

    Plasmid
    #75153
    Purpose
    Membrane targeted mVenus (yellow) fluorophore for use with 5’ promoters.
    Depositor
    Nicholas Cole
    Publication
    Don et al Zebrafish. 2016 Sep 15.
    Insert
    mVenus-CAAX
    Use
    Middle entry vector for multisite gateway three f…
    Promoter
    n/a
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    75153-plasmid-map-sequence-id-142107

    Enlarge

  13. pEF5/FRT/DEST-3xHA/mGli3/Flag P5-6A

    Plasmid
    #51249
    Purpose
    Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 980,1006 mutated to Ala
    Depositor
    Rajat Rohatgi
    Publication
    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gli3 (Gli3 Mouse)
    Use
    Flpin system
    Tags
    3xHA-TEV and Flag
    Expression
    Mammalian
    Mutation
    amino acids 980,1006 mutated to Ala
    Promoter
    EF1a
    Available since
    Feb. 28, 2014
    Availability
    Academic Institutions and Nonprofits only

  16. p3E-EGFP-NS-MCS

    Plasmid
    #75180
    Purpose
    EGFP (green) fluorophore with multiple cloning site for use with inducible N-terminal fusions.
    Depositor
    Nicholas Cole
    Publication
    Don et al Zebrafish. 2016 Sep 15.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP-NS-MCS
    Use
    Three prime entry vector for multisite gateway th…
    Promoter
    n/a
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    75180-plasmid-map-sequence-id-153030

    Enlarge

  18. p3E-mCherry-NS-MCS

    Plasmid
    #75179
    Purpose
    mCherry (red) fluorophore with multiple cloning site for use with inducible N-terminal fusions.
    Depositor
    Nicholas Cole
    Publication
    Don et al Zebrafish. 2016 Sep 15.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-NS-MCS
    Use
    Three prime entry vector for multisite gateway th…
    Promoter
    n/a
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    75179-plasmid-map-sequence-id-142186

    Enlarge

  19. pME-mKOFP2-CAAX

    Plasmid
    #75155
    Purpose
    Membrane targeted KOFP2 (orange) fluorophore for use with 5’ promoters.
    Depositor
    Nicholas Cole
    Publication
    Don et al Zebrafish. 2016 Sep 15.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mKOFP2-CAAX
    Use
    Middle entry vector for multisite gateway three f…
    Promoter
    n/a
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    75155-plasmid-map-sequence-id-145998

    Enlarge

Showing: 901 - 920 of 947 results