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TL026_AAV_hSyn:SEP-GluA1

Plasmid

#192519

Purpose

Expression of AMPA GluA1 with N terminal super ecliptic phluorin (SEP) fusion

Depositor

Adam Cohen

Article

Kim et al bioRxiv. 2023 May 29:2023.05.26.541296.

Insert

SEP-GluA1

Use

AAV and Cre/Lox

Tags

super ecliptic phluorin

Promoter

hSyn

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pSLIK 3xFLAG-TWIST2 neo

Plasmid

#197936

Purpose

Lentiviral expression vector for an inducible 3xFLAG-tagged TWIST2

Depositor

Kevin Janes

Article

Dutta et al Genome Res. 2023 Feb 21;33(3):314-31. do

Insert

TWIST2 (TWIST2 Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNAHygro(+)LAMP2B

Plasmid

#86029

Purpose

Expresses LAMP2B in Mammalian cells

Depositor

Janice Blum

Article

Zhou et al Immunity. 2005 May;22(5):571-81.

Insert

Lysosome-associated membrane protein 2b (LAMP2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_B2515

Plasmid

#91072

Purpose

Module B, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Esp3I ccdb cassette for single gRNA spacer cloning

Use

CRISPR

Promoter

AtU6

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab6ba

Plasmid

#109653

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab6ba

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab6ba (rab6ba Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNAHygro(+)LAMP2AS

Plasmid

#86146

Purpose

Anti sense for LAMP2

Depositor

Janice Blum

Article

Zhou et al Immunity. 2005 May;22(5):571-81.

Insert

LAMP2a (LAMP2 Human)

Expression

Mammalian

Mutation

contains first 525 bases of LAMP cDNA

Promoter

CMV

Available Since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

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lentiTet-P2A-mCherry BlastR

Plasmid

#186743

Purpose

Dox inducible P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene

Depositor

Richard Sherwood

Article

Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

Insert

P2A-mcherry

Use

Lentiviral

Expression

Mammalian

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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Cas9-GFP_sg_mAMPKa1

Plasmid

#79004

Purpose

Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 1.

Depositor

Reuben Shaw

Article

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Insert

Prkaa1 (Prkaa1 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pCRII-TOPO CMV-cGFP-mMALAT1_3' Comp.15 Sense

Plasmid

#46839

Purpose

Express cGFP transcript ending in a mutant MALAT1 triple helix that causes the transcript to be stable but poorly translated

Depositor

Phil Sharp

Article

Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

Insert

Coral Green Fluorescent Protein (cGFP)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/AUG-nLuc-3XFLAG

Plasmid

#127299

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2019

Availability

Academic Institutions and Nonprofits only

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Stat3 Flag pRc/CMV

Plasmid

#8707

Depositor

Jim Darnell

Article

Horvath et al Genes Dev 1995 Apr 15;9(8):984-94.

Insert

Stat3 (Stat3 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

*see comments

Available Since

Sept. 26, 2005

Availability

Academic Institutions and Nonprofits only

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Intron-Tagging-EGFP-Donor

Plasmid

#159740

Purpose

Contains EGFP flanked by a splice acceptor and a splice donor. Together with other intron tagging plasmids, it can be used to place the EGFP tag as a synthetic exon into introns of target genes.

Depositor

Stefan Kubicek

Article

Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

Insert

EGFP

Use

Intron tagging donor

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pDULE-ABK

Plasmid

#49086

Purpose

A pyrrolysyl tRNA/tRNA sythetase pair evolved to efficiently incorporate an aliphatic diazirine amino acid into proteins in E. coli and mammalian cells for crosslinking experiments

Depositor

Peter Schultz

Article

Ai et al Chembiochem. 2011 Aug 16;12(12):1854-7.

Inserts

tRNA synthetase
tRNA for a photocrosslinking amino acid

Expression

Bacterial

Mutation

L274M, C313A, Y349F

Promoter

GlnRS and lpp

Available Since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only

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Cas9-GFP_sg_mAMPKa2

Plasmid

#79005

Purpose

Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 2.

Depositor

Reuben Shaw

Article

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Insert

Prkaa2 (Prkaa2 Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pIS1-Eef25UTR-renilla

Plasmid

#38235

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

Eef2 5'UTR / Renilla (Eef2 Mouse)

Use

Luciferase

Expression

Mammalian

Mutation

Eef2 5' UTR fused to renilla ORF

Promoter

Endogenous Eef2

Available Since

Nov. 7, 2012

Availability

Academic Institutions and Nonprofits only

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pK.myc-MYPT1

Plasmid

#24101

Depositor

Masumi Eto

Article

Eto et al Cell Motil Cytoskeleton. 2005 Oct . 62(2

Insert

Myosin-targeting subunit-1 (PPP1R12A Human)

Tags

Myc

Expression

Mammalian

Mutation

S50G, P415Q

Available Since

March 19, 2010

Availability

Academic Institutions and Nonprofits only

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pIS1-Eef25UTR-TOPmut-renilla

Plasmid

#38236

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

Eef2_TOPm_5UTR/Renilla (Eef2 Mouse)

Use

Luciferase

Expression

Mammalian

Mutation

The first nucleotides of the 5' UTR were cha…

Promoter

Endogenous Eef2

Available Since

Nov. 7, 2012

Availability

Academic Institutions and Nonprofits only
pDIRECT_23A

Plasmid

#91138

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:bar

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:AtCas9 + AtU6:gRNA

Use

CRISPR

Expression

Plant

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pETM33_YES1_SH3

Plasmid

#178492

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

YES1_SH3 (YES1 Human, Synthetic)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-CRY2-iSH2

Plasmid

#66839

Purpose

Expression of a light-inducible PI3-kinase, generated by fusing the iSH2 domain of human p85α (residues 420–615) to mCherry-CRY2. Binds endogenous PI3-kinase p110α catalytic subunits constitutively.

Depositor

Pietro De Camilli

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

iSH2

Tags

mCherry-CRY2

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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