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pRK5_mEGFP-YAP-MAMLD1

Plasmid

#237675

Purpose

For overexpression of mEGFP-YAP-MAMLD1

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

YAP-MAMLD1 (YAP1, MAMLD1 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCHW110032

Plasmid

#222438

Purpose

Level 0, promoter module (GGAG-AATG) containing CBS4 and CMV1 promoters.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Insert

CBS4-CMV1, , CBS4 promoter + CMV1 (Cucumber mosaic virus)

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCHW110033

Plasmid

#222441

Purpose

Level 0, CDS module (AATG-GCTT) containing Cre recombinase.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Insert

Cre recombinase

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCSIIbsr-Eevee-ROCK-NES

Plasmid

#209918

Purpose

A FRET biosensor for ROCK activity

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

Eevee-ROCK-NES

Use

Lentiviral

Tags

ECFP and YPet

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast CHKA

Plasmid

#218540

Purpose

human CHKA cDNA

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

CHKA (CHKA Human)

Use

Retroviral

Expression

Mammalian

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3_CDC50A

Plasmid

#203694

Purpose

expresses CDC50A

Depositor

Peter Vangheluwe

Article

Martin et al Acta Neuropathol. 2020 Jun;139(6):1001-1

Insert

TMEM30A (TMEM30A Human)

Expression

Mammalian

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DIO-GRAB_NTS1.0

Plasmid

#208671

Purpose

Expresses the genetically-encoded fluorescent neurotensin (NTS) sensor GRAB_NTS1.0 in a cre-dependent manner

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based neurotensin (NTS) sensor GRAB_NTS1.0

Use

AAV

Promoter

EFS

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Tbg-CES2A-ΔC

Plasmid

#200558

Purpose

Secreted mouse CES2A driven by heptaocyte-specific promoter in AAV viral vector

Depositor

Jonathan Long

Article

Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

Insert

Mouse Carboxylesterase 2A delta C-terminal (Ces2a Mouse)

Use

AAV

Tags

Flag

Promoter

TBG promoter

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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KIF1C

Plasmid

#155687

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

KIF1C (KIF1C Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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W118-1_hTGM4-Flag

Plasmid

#180409

Purpose

lentiviral transduction of human TGM4 (with a C-terminal Flag tag) gene

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

TGM4 (TGM4 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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W118-1_hTGM5-V5

Plasmid

#180414

Purpose

lentiviral transduction of human TGM5 (with a C-terminal V5 tag) gene

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

TGM5 (TGM5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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CDC40

Plasmid

#155444

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CDC40 (CDC40 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCold I-Hero7

Plasmid

#187926

Purpose

Bacterial expression of heat-resistant obscure (Hero) protein Hero7

Depositor

Yukihide Tomari

Article

Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

Insert

Hero7 (SERF2 Human)

Tags

Flag-6xHis

Expression

Bacterial

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF350

Plasmid

#88526

Purpose

Donor Vector containing ZNF350 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF350 (ZNF350 Human)

Use

Gateway Cloning

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF655

Plasmid

#88578

Purpose

Donor Vector containing ZNF655 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF655 (ZNF655 Human)

Use

Gateway Cloning

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF785

Plasmid

#88459

Purpose

Donor Vector containing ZNF785 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF785 (ZNF785 Human)

Use

Gateway Cloning

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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mEm-CoilE-Actin

Plasmid

#181993

Purpose

CoilE genetically encoded tag on mEmerald-Actin, to be used with CoilR labeled with a reporter

Depositor

Kimberly Beatty

Article

Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

Insert

mEm-CoilE-Actin

Tags

mEmerald

Expression

Mammalian

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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Sc eRF3∆165N (WT)

Plasmid

#175418

Purpose

Encodes His-MBP-TEV-WT eRF3∆165N

Depositor

Joseph Puglisi

Article

Lawson et al Science. 2021 Aug 20;373(6557):876-882.

Insert

eRF3 (SUP35 Budding Yeast)

Tags

His-MBP-TEV

Expression

Bacterial

Mutation

∆165N

Promoter

T7

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF449

Plasmid

#88453

Purpose

Donor Vector containing ZNF449 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF449 (ZNF449 Human)

Use

Gateway Cloning

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223-ZNF770

Plasmid

#88562

Purpose

Donor Vector containing ZNF770 transcription factor, part of the Human TFome Collection

Depositor

Marc Vidal

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF770 (ZNF770 Human)

Use

Gateway Cloning

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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