We narrowed to 18,351 results for: multi
-
Plasmid#104607PurposeExpresses TALE3 under UAS controlDepositorInsertTALE3
UseTALENExpressionInsectPromoterhsp70Available sinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUC CBA-SpCas9N863Anickase.EF1a-BFP.sgLMNApair1
Plasmid#98976PurposeCas9 Homologous Recombination Reporter. SpCas9N863A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 44bp 3' overhangs. TagBFP.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLMNA sgRNAs pair 1 and Cas9(N863A)
ExpressionMammalianAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair1
Plasmid#98972PurposeCas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNA targeting hLMNA. Generates breaks with 44bp 5' overhangs. TagBFP.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLMNA sgRNAs pair 1 and Cas9(D10A)
ExpressionMammalianAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCXCR4_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
Plasmid#101113PurposeEncodes dCas9(C)-VP64 with SAM components fused to CXCR4DepositorInsertCXCR4-dCas9(C)-VP64
UseCRISPR and Synthetic BiologyTagsFlag and HAExpressionMammalianAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
OeNL-H2B_pcDNA3
Plasmid#89527PurposeOrange color variant of bright luminescent protein, fused to histone 2BDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertOeNL
Tagshistone 2BExpressionMammalianPromoterCMVAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
Cox8x2-OeNL_pcDNA3
Plasmid#89526PurposeOrange color variant of bright luminescent protein, targeted to the MitochondriaDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertOeNL
TagsCoxVIII X2ExpressionMammalianPromoterCMVAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
LAMP-OeNL_pcDNA3
Plasmid#89525PurposeOrange color variant of bright luminescent protein, fused to Lysosomal-associated membrane protein 1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertOeNL
TagsLAMP1ExpressionMammalianPromoterCMVAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
YeNL-Actin_pcDNA3
Plasmid#89521PurposeYellow color variant of bright luminescent protein, fused to actinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertYeNL
TagsActinExpressionMammalianPromoterCMVAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
tubulin-YeNL_pcDNA3
Plasmid#89519PurposeYellow color variant of bright luminescent protein, fused to b-tubulinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertYeNL
Tagsb-tubulinExpressionMammalianPromoterCMVAvailable sinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-013
Plasmid#78701PurposeA weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a strong RBS.DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
UseSynthetic BiologyAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACYC-Skp-hLYZ
Plasmid#83924PurposeCo-expresses cytoplasmic copies of the Skp protein folding chaperone and wild type human lysozyme..DepositorAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFastBacDual with LFn + PrgJ
Plasmid#84869PurposeBaculovirus Expression of LFn-PrgJ fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel proteinDepositorInsertsLFn
PrgJ
UseBaculovirusTags6xHisExpressionInsectPromoterPolyhedrinAvailable sinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-SS-267
Plasmid#68770PurposeSensor pair 1 V1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsC-terminal Sensor 1 V1
N-Terminal Sensor 1 V1
TagsNLS, flag, and nlsExpressionMammalianAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
XW20
Plasmid#65836PurposePunc-4-QF-3'UTR-unc-54 (The endogenous SphI, KpnI and SacI sites in QF are removed, and no SL2-mcherry)DepositorInsertOR74A quinic acid utilization activator (qa-1F)
ExpressionWormMutationThe endogenous SphI, KpnI and SacI sites in QF ar…Available sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC1-pCR8-dCas9VP48
Plasmid#48214PurposedCas9VP48 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expressionDepositorInsertdCas9(D10A;H840A) fusion with VP48 activation domain
UseCRISPR and Gateway CloningTagsHA Tag and VP48MutationD10A;H840AAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
Rc/CMV cyclin D2 LxCxE HA
Plasmid#8957DepositorPublicationAvailable sinceOct. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
-
pAAV-hSyn-con/fon DREADD Gi-mCherry
Plasmid#200660PurposeConstruct for chemogenetically silencing and visualizing subpopulations of neurons in the retinaDepositorInsertDREADD Gi-mCherry
UseAAV, Cre/Lox, and Mouse Targeting ; IntrsectTagsmCherryExpressionMammalianPromoterhSynAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-H3.3(WT)-3AID-HA-2A-mTurquoiseBSD
Plasmid#225701PurposeLentiviral expression of AID degron tagged Wildtype H3.3 and mTurquoise fused BlasticidinRDepositorUseLentiviralTags3xAID HA and T2A-mTurquoiseExpressionMammalianAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
HuEx-1xNLS-iNme2Cas9-npNLS_EGFP-T1
Plasmid#222992PurposeMammalian expression of iNme2Cas9 construct with EGFP-targeting sgRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHuEx-1xNLS-iNme2Cas9-npNLS_EGFP-T1
UseCRISPRTagsPuroExpressionMammalianPromoterpCAGAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only