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PB_TRE_AP4-PDGFR_EF1a_eBFP2

Plasmid

#186306

Purpose

Plasmid for inducible mammalian cell expression of the AP4 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

AP4-PDGFR
eBFP2

Tags

HA, myc

Expression

Mammalian

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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PB_TRE_sg83-PDGFR_EF1a_iRFP670

Plasmid

#186311

Purpose

Plasmid for inducible mammalian cell expression of the sg83 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line construction

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

sg83-PDGFR
iRFP670

Tags

His, myc

Expression

Mammalian

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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PB_TRE_sg88-PDGFR_EF1a_eBFP2

Plasmid

#186312

Purpose

Plasmid for inducible mammalian cell expression of the sg88 helixCAM and constitutive expression of an identifying fluorescent protein (eBFP2). Flanked with PiggyBac sites for easy line construction.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

sg88-PDGFR
eBFP2

Tags

His, myc

Expression

Mammalian

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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PB_TRE_P9-PDGFR_EF1a_mPlum

Plasmid

#186307

Purpose

Plasmid for inducible mammalian cell expression of the P9 helixCAM and constitutive expression of an identifying fluorescent protein (mPlum). Flanked with PiggyBac sites for easy line construction.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

P9-PDGFR
mPlum

Tags

HA, myc

Expression

Mammalian

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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PB_TRE_AP10-PDGFR_EF1a_mOrange

Plasmid

#186308

Purpose

Plasmid for inducible mammalian cell expression of the AP10 helixCAM and constitutive expression of an identifying fluorescent protein (mOrange). Flanked with PiggyBac sites for easy line construction

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

AP10-PDGFR
mOrange

Tags

HA, myc

Expression

Mammalian

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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PB_TRE_P3-PDGFR_EF1a_iRFP670

Plasmid

#186305

Purpose

Plasmid for inducible mammalian cell expression of the P3 helixCAM and constitutive expression of an identifying fluorescent protein (iRFP670). Flanked with PiggyBac sites for easy line construction.

Depositor

George Church

Article

Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

Inserts

P3-PDGFR
iRFP670

Tags

HA, myc

Expression

Mammalian

Available Since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLL3.7m-YFP-SLBP(18-126)-IRES-H1-Skylan62A

Plasmid

#205760

Purpose

Single-color FUCCI4 sensor for S/G2 cell cycle transition and G2/M transition

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

YFP-SLBP(18-126)-IRES-H1-Skylan62A

Use

Lentiviral

Expression

Mammalian

Mutation

wt

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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HA-EcPPK1

Plasmid

#159279

Purpose

Expresses a HA-tagged E. coli PPK1 in mammalian cell lines

Depositor

Michael Downey

Article

Bondy-Chorney et al Cell Rep. 2020 Oct 27;33(4):108318. doi:

Insert

HA-tagged Polyphosphate Kinase 1

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pGENE:EFF-1::mCherry::V5

Plasmid

#133020

Purpose

EFF-1 fused to mCherry fluorescent protein cloned into the pGene vector for inducible expression in mammalian cells

Depositor

Benjamin Podbilewicz

Article

Perez-Vargas et al Cell. 2014 Apr 10;157(2):407-419. doi: 1

Insert

EFF-1::mCherry::V5

Tags

V5; mCherry

Expression

Mammalian

Available Since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pSADB19-dTomato Barcode Library

Pooled Library

#227671

Purpose

RVdG genome plasmids that carry an error-correctable barcode compatible with 3’ capture single cell or single nuclei RNA sequencing readout. For the production of SADB19 strain G-deleted rabies virus.

Depositor

Cathryn Cadwell, Tomasz Nowakowski

Article

Shin et al bioRxiv [Preprint]. 2024 Dec 11:2024.10.

Expression

Mammalian

Species

Synthetic

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only
Oxford Drosophila melanogaster Genome-wide CRISPR Library

Pooled Library

#64750

Purpose

Library designed for genomic targeting and screening in Drosophila cells. All possible gRNA target sites within the shared exonic regions on either DNA strand of the Drosophila genome were computed.

Depositor

Ji-Long Liu

Article

Bassett et al J Genet Genomics. 2015 Jun 20;42(6):301-

Expression

Insect

Species

Drosophila melanogaster, Streptococcus pyogenes, and Synthetic

Use

CRISPR

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only
SARS-CoV-2 Spike (S) Ectodomain Library C

Pooled Library

#157973

Purpose

Encodes Spike ectodomain mutations that stabilize 'up' prefusion conformation

Depositor

Timothy Whitehead

Article

Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

Expression

Bacterial and Yeast

Species

Sars-cov-2

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only
SARS-CoV-2 Spike (S) Ectodomain Library B

Pooled Library

#157972

Purpose

Encodes Spike ectodomain mutations that stabilize 'up' prefusion conformation

Depositor

Timothy Whitehead

Article

Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

Expression

Bacterial and Yeast

Species

Sars-cov-2

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only
SARS-CoV-2 Spike (S) Ectodomain Library A

Pooled Library

#157971

Purpose

Encodes Spike ectodomain mutations that stabilize 'up' prefusion conformation

Depositor

Timothy Whitehead

Article

Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

Expression

Bacterial and Yeast

Species

Sars-cov-2

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only
BLaER1 pgRNA CRISPR library

Pooled Library

#183825

Purpose

Paired gRNA library targeting genes related to B-cell to macrophage transdifferentiation.

Depositor

Roderic Guigo

Article

Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available Since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only
pCMMP-MCS-IRES-eGFP

Plasmid

#36953

Depositor

Bill Sugden

Article

Kennedy et al Mol Cell Biol. 2003 Oct;23(19):6901-8.

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2012

Availability

Academic Institutions and Nonprofits only

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mTFP1-mKO-FRET-8

Plasmid

#58152

Purpose

Localization: Cytosolic FRET, Excitation: 462 / 548, Emission: 492 / 561

Depositor

Michael Davidson

Type

Empty backbone

Tags

mKO and mTFP1

Expression

Mammalian

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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PS-CFP2-mCherry-FRET-10

Plasmid

#58157

Purpose

Localization: Cytosolic FRET, Excitation: 490 / 587, Emission: 511 / 610

Depositor

Michael Davidson

Type

Empty backbone

Tags

PS-CFP2 and mCherry

Expression

Mammalian

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only
U6+27-cxcr4

Plasmid

#22498

Depositor

Phil Sharp

Article

Ebert et al Nat Methods. 2007 Sep . 4(9):721-6.

Type

Empty backbone

Expression

Mammalian

Available Since

Nov. 12, 2009

Availability

Academic Institutions and Nonprofits only
U6+27-20

Plasmid

#21977

Depositor

Phil Sharp

Article

Ebert et al Nat Methods. 2007 Sep . 4(9):721-6.

Insert

U6 miR-20 sponge

Expression

Mammalian

Available Since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only