We narrowed to 21,065 results for: Cre

Showing: 9261 - 9280 of 21065 results

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pLV_hU6-sgRNA_hUbC-dSaCas9-KRAB-T2A-PuroR

Plasmid

#162334

Purpose

Lentiviral expression of dSaCas9-KRAB and a sgRNA with puromycin resistance

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

dSaCas9-KRAB

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

hUbC

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-AncBE4max

Plasmid

#174164

Purpose

This is an AncBE4max (a cytosine base editor) expressing plasmid built on the 3rd generation system of lentiviral vector. It can be used for stable expression of AncBE4max in cells.

Depositor

Wensheng Wei

Article

Xu et al Nat Biotechnol. 2021 Jun 21. pii: 10.103

Insert

AncBE4max

Use

Lentiviral

Tags

P2A-mCherry

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pAct-FRT-stop-FRT3-FRT-FRT3-Gal4 attB

Plasmid

#52889

Purpose

"CoinFLP-Gal4" - Expresses Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express Gal4, or FRT3-FRT3 pair to prevent Gal4 expression

Depositor

Iswar Hariharan

Article

Bosch et al Development. 2015 Feb 1;142(3):597-606.

Insert

Gal4 (GAL4 Budding Yeast)

Expression

Insect

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pCashGem-Xyl2-URA3-LINEAR

Plasmid

#174840

Purpose

pRS414 backbone containing LINEAR fragment targeting XYL2 in S. stipitis

Depositor

Zengyi Shao

Article

Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

Insert

Codon optimized Cas9:hGem

Use

CRISPR and Synthetic Biology

Tags

hGeminin tag

Expression

Bacterial and Yeast

Promoter

ENO1p

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-PKM2-WT

Plasmid

#122690

Purpose

Express His-tagged PKM2 (wild type) protein in bacteria.

Depositor

Wen-Ching Wang

Article

Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

Insert

Pyruvate kinase M2 isoform (PKM Human)

Tags

6His-tag

Expression

Bacterial

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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IRE1 alpha-pcDNA3.EGFP

Plasmid

#13009

Depositor

Fumihiko Urano

Article

Lipson et al Cell Metab. 2006 Sep . 4(3):245-54.

Insert

Inositol Requiring Enzyme-1 (ERN1 Human)

Expression

Mammalian

Available Since

Nov. 27, 2006

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_TNF-SBP-EGFP

Plasmid

#65278

Purpose

synchronize trafficking of TNF from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

TNF (TNF Human)

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Promoter

CMV

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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AV4-EF1-NMNAT2

Plasmid

#124970

Purpose

Express NMNAT2

Depositor

Kazuhiro Oka

Article

Ljungberg et al Hum Mol Genet. 2012 Jan 15;21(2):251-67.

Insert

NMNAT2, GFP (NMNAT2 Human)

Use

AAV

Promoter

EF1-alpha

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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SSTR2-DuET

Plasmid

#213377

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

SSTR2 (SSTR2 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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mKate2-P2A-APEX2-GBP

Plasmid

#67650

Purpose

Mammalian expression vector driving APEX2-GBP (EM peroxidase marker fused to GFP-targeting nanobody). Also contains bicistronially expressed cytoplasmic red fluorescent reporter.

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

APEX2-GBP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti6/V5-p53_R249S

Plasmid

#22935

Depositor

Bernard Futscher

Article

Junk et al Neoplasia. 2008 May . 10(5):450-61.

Insert

tumor protein 53 (TP53 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

R249S Mutant

Available Since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only

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pEGFP VAMP8

Plasmid

#42311

Depositor

Thierry Galli

Article

Paumet et al J Immunol. 2000 Jun 1;164(11):5850-7.

Insert

VAMP 8 (VAMP8 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2013

Availability

Academic Institutions and Nonprofits only

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HotPETase_pET21b

Plasmid

#215407

Purpose

Expression construct for HotPETase gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

HotPETase

Expression

Bacterial

Mutation

S121E/D186H/R280A/P181V/S207R/S214Y/Q119K/S213E/N…

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCW-tTRE-scFv-SunTag

Plasmid

#193138

Purpose

Expresses scFv intracellular antibody component of the SunTag system under Dox regulation

Depositor

Brad Rosenberg

Article

Danziger et al PLoS Pathog. 2022 Apr 14;18(4):e1010464.

Insert

scFv-SunTag

Use

Lentiviral

Expression

Mammalian

Promoter

tight TRE promoter

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-REPORT(PGK)

Plasmid

#172328

Purpose

LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an PGK promoter driving nuclear d2eGFP E2A Hygromycin

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Inserts

monomeric nuclear Tomato
d2eGFP
HygR

Use

Lentiviral and Synthetic Biology

Tags

3x SV40 NLS

Expression

Mammalian

Promoter

PGK

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-Myc-Parkin

Plasmid

#17612

Depositor

Ted Dawson

Article

Zhang et al Proc Natl Acad Sci U S A 2000 Nov 21;97(

Insert

Parkin (PRKN Human)

Tags

Myc

Expression

Mammalian

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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pLenti6/V5-p53_R280K

Plasmid

#22933

Depositor

Bernard Futscher

Article

Junk et al Neoplasia. 2008 May . 10(5):450-61.

Insert

tumor protein 53 (TP53 Human)

Use

Lentiviral

Tags

v5

Expression

Mammalian

Mutation

R280K mutant

Available Since

Jan. 27, 2010

Availability

Academic Institutions and Nonprofits only
p2,0_3M5M_eGFP

Plasmid

#135474

Purpose

Encodes Marburg virus minigenome with eGFP reporter gene under control of a T7 RNA polymerase promoter

Depositor

Elke Mühlberger

Article

Manhart et al Cell Rep. 2018 Sep 4;24(10):2573-2580.e4

Insert

Marburg virus minigenome, eGFP reporter

Expression

Mammalian

Mutation

None

Promoter

T7

Available Since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-PKM2-R399E

Plasmid

#122691

Purpose

Express His-tagged PKM2 (R399E) protein in bacteria.

Depositor

Wen-Ching Wang

Article

Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

Insert

Pyruvate kinase M2 isoform (PKM Human)

Tags

6His-tag

Expression

Bacterial

Mutation

R399E

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pGL4.10-VEGFprom(-1000 to -1)

Plasmid

#66128

Purpose

Luciferase-based reporter for VEGF promoter region (-1000 to -1 bp)

Depositor

David Mu

Article

Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

Insert

Vascular Endothelial Growth Factor -A promoter region (VEGFA Human)

Use

Luciferase

Promoter

VEGF -1000 to -1

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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