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pDest-312 (attR4-attR3)

Plasmid

#161897

Purpose

Multisite Gateway (attR4-attR3) vector for mammalian protein expression

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Expression

Mammalian

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pSEL2

Plasmid

#23012

Depositor

James Bowie

Article

Massey-Gendel et al Protein Sci. 2009 Feb . 18(2):372-83.

Insert

Kanamycin resistance

Tags

6 His Tag and Xpress epitope

Expression

Bacterial

Available Since

Feb. 17, 2010

Availability

Academic Institutions and Nonprofits only
pID3.01B

Plasmid

#22720

Depositor

Geraldine Seydoux

Article

D'Agostino et al Dev Biol. 2006 Apr 1. 292(1):244-52.

Insert

unc-119 rescuing fragment / pie-1promoter-GFP- gateway destination cassette B -pie-1 3’

Expression

Bacterial

Available Since

Jan. 21, 2010

Availability

Academic Institutions and Nonprofits only

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GGE080

Plasmid

#120774

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for position 3 (T3_Lip2)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

T3_Lip2

Use

Cloning/assembly plasmid

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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LI A-B pNOS c1

Plasmid

#54456

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

nopaline synthase promoter

Use

Cloning vector

Available Since

June 18, 2014

Availability

Academic Institutions and Nonprofits only

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pDest-302 (attR4-attR2)

Plasmid

#161894

Purpose

Multisite Gateway (attR4-attR2) vector for mammalian protein expression

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Expression

Mammalian

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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YIplac211-iGFP-TLG2

Plasmid

#105262

Purpose

Plasmid for pop-in/pop-out replacement of Tlg2 with iGFP-Tlg2.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Tlg2 (TLG2 Budding Yeast)

Tags

iGFP

Expression

Yeast

Available Since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-ARF1-msGFP

Plasmid

#105255

Purpose

Plasmid for pop-in/pop-out replacement of Arf1 with Arf1-msGFP.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Arf1 (ARF1 Budding Yeast)

Tags

msGFP

Expression

Yeast

Available Since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCHAP4

Plasmid

#206850

Purpose

A plasmid containing a 16,121 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector.

Depositor

Bogumil Karas

Article

Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pPt_Cp

Plasmid

#206855

Purpose

A plasmid containing the whole P. tricornutum chloroplast genome, cloned with a pCC1BAC-based backbone. The marker URA3 was integrated into the genome.

Depositor

Bogumil Karas

Article

Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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