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pRS315-SEC22-UWOPS872421

Plasmid

#226272

Purpose

Plasmid expressing the SEC22 allele from UWOPS87-2421, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SEC22 (SEC22 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pRS315-SEC22-S288C

Plasmid

#226270

Purpose

Plasmid expressing the SEC22 allele from S288C, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SEC22 (SEC22 Budding Yeast)

Expression

Yeast

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pML107-PMR1

Plasmid

#226264

Purpose

Plasmid expressing Cas9 and gRNA ACATGACCGTATCTAAACTT which targets the PMR1 gene

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

PMR1 gRNA (PMR1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pRS316-SSD1-NCYC110

Plasmid

#226279

Purpose

Plasmid expressing the SSD1 allele from NCYC110, under control of its native promoter

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

SSD1 (SSD1 Budding Yeast)

Expression

Yeast

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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gRNA_SRR134.3_miRFP670

Plasmid

#163754

Purpose

gRNA expression vector containing the miRFP670 fluorescent marker to target the region downstream of the SRR134 SOX2 enhancer in human cells.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

gRNA_SRR134.3

Use

CRISPR

Tags

miRFP670

Expression

Mammalian

Promoter

U6

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pBa.PEX 1-42-tdTM-FKBP

Plasmid

#224415

Purpose

Mammalian expression of Pex3

Depositor

Marvin Bentley

Article

Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

Insert

Pex3 (Pex3 Mouse)

Tags

tdTom-FKBP

Expression

Mammalian

Promoter

Chicken Beta actin

Available Since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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300_pETcon_SARS2_FLip

Plasmid

#222230

Purpose

yeast surface display of the SARS-CoV-2 FLip variant RBD

Depositor

Tyler Starr

Article

Taylor et al Virus Evol. 2024 Sep 2;10(1):veae067. do

Insert

SARS-CoV-2 FLip RBD (S SARS-CoV-2 virus, Budding Yeast)

Tags

HA and c-Myc

Expression

Yeast

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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297_pETcon_SARS2_Omicron-BA286

Plasmid

#222231

Purpose

yeast surface display of the SARS-CoV-2 BA.2.86 variant RBD

Depositor

Tyler Starr

Article

Taylor et al Virus Evol. 2024 Sep 2;10(1):veae067. do

Insert

SARS-CoV-2 BA.2.86 RBD (S SARS-CoV-2 virus, Budding Yeast)

Tags

HA and c-Myc

Expression

Yeast

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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299_pETcon_SARS2_EG-5

Plasmid

#222232

Purpose

yeast surface display of the SARS-CoV-2 EG.5 variant RBD

Depositor

Tyler Starr

Article

Taylor et al Virus Evol. 2024 Sep 2;10(1):veae067. do

Insert

SARS-CoV-2 EG.5 RBD (S SARS-CoV-2 virus, Budding Yeast)

Tags

HA and c-Myc

Expression

Yeast

Available Since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pTarget-Dual

Plasmid

#207884

Purpose

Carries the cognate target sites for the homing endonucleases I-OnuI and I-GpeMI.

Depositor

David Edgell

Article

Foo et al Nucleic Acids Res. 2024 Feb 28;52(4):206

Inserts

I-OnuI target site
I-GpeMI target site

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pAGK43

Plasmid

#222223

Purpose

Edits NPAS2 Gene.

Depositor

Seth Shipman

Article

Crawford et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

Insert

Eco1 RT; 112 nt donor; PAM -19 (NPAS2 Human)

Use

CRISPR

Expression

Mammalian

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pRAR119

Plasmid

#185051

Purpose

J23108(spel), pHP14 stability hairpin, strong RBS, L34 coding region, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L34

Expression

Bacterial

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pAcGFP-N1_CBPdIDR7KTG

Plasmid

#221915

Purpose

Transient expression of GFP-tagged CBPdeltaIDR7KTG in mammalian cells

Depositor

Daniel Bose

Insert

CBP (CREBBP Human)

Tags

GFP

Expression

Mammalian

Mutation

deletion of IDR7 (aa 2109-2434); mutation of lysi…

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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pC0.378

Plasmid

#203937

Purpose

Down Flank. Neutral site. Down flanking sequence of ω3 acyl-lipid desaturase (desB, FEK30_04840) in Synechococcus sp. PCC 11901.

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

desB DOWN

Use

Synthetic Biology

Available Since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pC0.438

Plasmid

#203943

Purpose

Down Flank. Neutral site. Up flanking sequence of intergenic region (NS1) between FEK30_11550 and FEK30_11555 in Synechococcus sp. PCC 11901.

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

NS1 DOWN

Use

Synthetic Biology

Available Since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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pRH3128

Plasmid

#210165

Purpose

Protein quality control substrate and associated control YIp ADE2-LEU2 pTDH3::ADE1-GFP::tADH1

Depositor

Randy Hampton

Article

Flagg et al Mol Biol Cell. 2023 Dec 1;34(13):ar125.

Insert

YIp ADE2-LEU2 pTDH3::ADE1-GFP::tADH1 (ADE2 Budding Yeast)

Expression

Yeast

Available Since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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gRNA-hIRF-1 #12/pSIR-hCD2

Plasmid

#135392

Purpose

Expresses a guide RNA (gRNA) to target human IRF-1 promoter

Depositor

Hodaka Fujii

Article

Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

Insert

gRNA_hIRF1 promoter #12

Use

CRISPR and Retroviral

Expression

Mammalian

Promoter

U6

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pzk490-pmax-NES-dpspCas13b(AAAA)-FseI[ADAR2-DD-splitC]

Plasmid

#196843

Purpose

Express NES-dpspCas13b(AAAA)-FseI[ADAR2-DD-splitC]

Depositor

Albert Cheng

Article

Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

Insert

NES-dpspCas13b(AAAA)-FseI[ADAR2-DD-splitC]

Expression

Mammalian

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO FLAG-RPS28

Plasmid

#203156

Purpose

N-FLAG-RPS28 in inducible pcDNA5 vector for Flp-In integration

Depositor

Susan Baserga

Article

Bryant et al Nucleic Acids Res. 2024 Feb 28;52(4):198

Insert

RPS28 (RPS28 Human)

Tags

1X FLAG tag with GGGGS linker

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-Vector

Plasmid

#214477

Purpose

Vector control for mCherry fusions. Created from the backbone of Addgene #55043 mCherry-Ezrin-N-14 from Michael Davidson.

Depositor

Natalie Ahn

Article

Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.

Insert

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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