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YSBE 596 : pNAT_mCherry

Plasmid

#92088

Purpose

Plasmid for tagging proteins in Cryptococcus neoformans; mCherry tag, NAt selection

Depositor

Yong-Sun Bahn

Article

So et al J Microbiol Biotechnol. 2017 Feb 28;27(2

Insert

mCherry

Use

tagging proteins in cryptococcus neoforman

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pT-35

Plasmid

#16092

Depositor

Yoshiko Kikuchi

Article

Takahashi et al J Biol Chem. 2001 Dec 28. 276(52):48973-

Insert

SMT3 (SMT3 Budding Yeast)

Tags

GST

Expression

Bacterial

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
RCASBP-AP (E) (CC#39)

Plasmid

#15162

Depositor

Connie Cepko

Article

Fekete et al Mol Cell Biol. 1993 Apr . 13(4):2604-13.

Insert

PLAP (ALPP Human)

Use

Retroviral; Avian expression

Available Since

June 8, 2007

Availability

Academic Institutions and Nonprofits only
pPOTv7-blast-mScarlet

Plasmid

#179818

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScarlet

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-g418-mNG

Plasmid

#179821

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNG

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPP384

Plasmid

#158799

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-3(D413A) in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

dCasPhi-3 (D413A)

Expression

Bacterial

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-RNF17S

Plasmid

#35414

Depositor

Jeremy Wang

Article

Pan et al Development. 2005 Sep;132(18):4029-39. E

Insert

Rnf17s (Rnf17 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 25, 2013

Availability

Academic Institutions and Nonprofits only
pT7-EGFP-C1-HsY14_I

Plasmid

#146583

Purpose

Mammalian Expression of HsY14

Depositor

Elisa Izaurralde

Article

Palacios et al Nature. 2004 Feb 19. 427(6976):753-7.

Insert

HsY14 (RBM8A Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSLQ2853-4 pHR: U6-Sasgv2CXCR4-2 CMV-EGFP

Plasmid

#84255

Purpose

Expresses optimized Sa sgCXCR4-2 gRNA with an EGFP fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sa sgCXCR4-2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-mScarletI

Plasmid

#201442

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScarlet-I

Use

Unspecified

Promoter

read through

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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mCherryC1-PIPKI{gamma}661

Plasmid

#29584

Depositor

Anna Huttenlocher

Article

Lokuta et al Mol Biol Cell. 2007 Dec . 18(12):5069-80

Insert

phosphatidylinositol phosphate kinase type I gamma

Tags

mCherry

Expression

Mammalian

Available Since

May 23, 2011

Availability

Academic Institutions and Nonprofits only

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p6661 MSCV-IP N-HA only 16E6

Plasmid

#42603

Depositor

Peter Howley

Article

White et al J Virol. 2012 Dec;86(24):13174-86. doi:

Insert

HPV16 E6

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

April 26, 2013

Availability

Academic Institutions and Nonprofits only
pFastBac FPH

Plasmid

#1925

Depositor

Robert Kingston

Article

Francis et al Mol Cell 2001 Sep;8(3):545-56.

Insert

polyhomeotic (ph-d, ph-p Fly)

Tags

Flag

Expression

Insect

Available Since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only
SNAP-EF(N)-60nmSAH-GFP(1-10)

Plasmid

#61663

Purpose

2x-30nm SAH scaffold with EF Hand and split GFP attachment sites

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

SNAP-EF(N)-60nmSAH-GFP(1-10)

Expression

Mammalian

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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pPOTv7-hygro-mScarletI

Plasmid

#179820

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScarlet-I

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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mEGFP-30nmSAH-GFP(1-10)

Plasmid

#61677

Purpose

GFP-labeled SAH scaffold with split GFP attachment site

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

mEGFP-30nmSAH-GFP(1-10)

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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pCellFree_G05

Plasmid

#67139

Purpose

Gateway destination vector for cell-free expression with N-term 8xHis mCherry tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

His and mCherry

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only
pXS-dTomato

Plasmid

#117387

Purpose

pGEN-mcs with a modular dTomato expression scaffold

Depositor

Karen Guillemin

Article

Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

Insert

dTomato

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptac

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pPD355 VPR-FRB (NLS)

Plasmid

#138847

Purpose

Constitutive expression of VPR-FRB under the CMV promoter.

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

VPR-FRB

Use

Synthetic Biology

Tags

3xFlag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pKM498

Plasmid

#134215

Purpose

Baculovirus expression of CENP-T and CENP-W

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

his-CENPT + CENPW

Expression

Insect

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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