-
Plasmid#160782PurposeSuppress ERCC2DepositorInsertshERCC2_2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P ERCC2_1
Plasmid#160781PurposeSuppress ERCC2DepositorInsertshERCC2_1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P DNA2_2
Plasmid#160780PurposeSuppress DNA2DepositorInsertshDNA2_2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P DNA2_1
Plasmid#160779PurposeSuppress DNA2DepositorInsertshDNA2_1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P CISD1_2
Plasmid#160774PurposeSuppress CISD1DepositorInsertshCISD1_2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P BRIP1_2
Plasmid#160772PurposeSuppress BRIP1DepositorInsertshBRIP1_2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1P BRIP1_1
Plasmid#160771PurposeSuppress BRIP1DepositorInsertshBRIP1_1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1B CDC25C_2
Plasmid#160770PurposeSuppress CDC25CDepositorInsertshCDC25C_2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1B CDC25C_1
Plasmid#160769PurposeSuppress CDC25CDepositorInsertshCDC25C_1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1B CDC25A_1
Plasmid#160767PurposeSuppress CDC25ADepositorInsertshCDC25A_1
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-aC-VTS-US
Plasmid#160425PurposeExpression of c-luciferase from sp. Vargula tsujii (USA)DepositorInsertluciferase
UseLuciferaseTags6x Histidine tag, Yeast alpha secretion factor, c…ExpressionYeastMutationSee depositor comments belowPromoterAOXAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPICZ-aC-MSVU-BZ
Plasmid#160426PurposeExpression of c-luciferase from sp. Maristella sp. SVU (Belize)DepositorInsertluciferase
UseLuciferaseTags6x Histidine tag, Yeast alpha secretion factor, c…ExpressionYeastMutationSee depositor comments belowPromoterAOXAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c2X-ORF24-NTD-3L_A
Plasmid#138466PurposeExpresses MBP-tagged ORF24-NTD 3L_A in bacteriaDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsMBPExpressionBacterialMutationAmino acids 1-201; amino acids L73-75 mutated to …PromotertacAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQLink-GST-2xCTD
Plasmid#138470PurposeExpresses GST-tagged 2xCTD heptapeptide repeat in bacteriaDepositorInsertRpb1 (POLR2A Human)
UseTagsGSTExpressionBacterialMutation2x CTD repeatPromoterT5Available sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF24 L73A_L75A-3xFLAG
Plasmid#138429PurposeExpresses full-length ORF24 L73A/L75A with a C-terminal 3xFLAG tag in mammalian cellsDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsFLAGExpressionMammalianMutationAmino acids L73, L75 mutated to alaninePromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF24 L74A_L75A-3xFLAG
Plasmid#138430PurposeExpresses full-length ORF24 L74A/L75A with a C-terminal 3xFLAG tag in mammalian cellsDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsFLAGExpressionMammalianMutationAmino acids L74, L75 mutated to alaninePromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF24 R68A-3xFLAG
Plasmid#138431PurposeExpresses full-length ORF24 R68A with a C-terminal 3xFLAG tag in mammalian cellsDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsFLAGExpressionMammalianMutationAmino acid R68 mutated to alaninePromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF24 R72A-3xFLAG
Plasmid#138432PurposeExpresses full-length ORF24 R72A with a C-terminal 3xFLAG tag in mammalian cellsDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsFLAGExpressionMammalianMutationAmino acid R72 mutated to alaninePromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF24 L73A_L74A-3xFLAG
Plasmid#138433PurposeExpresses full-length ORF24 L73A/L74A with a C-terminal 3xFLAG tag in mammalian cellsDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsFLAGExpressionMammalianMutationAmino acids L73, L74 mutated to alaninePromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-UL87 3L_A-2xStrep
Plasmid#138437PurposeExpresses full-length UL87 3L_A with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertUL87 (UL87 HCMV (HHV-5))
UseTagsStrepExpressionMammalianMutationAmino acids L90-L92 mutated to alaninePromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-BcRF1 3L_A-2xStrep
Plasmid#138439PurposeExpresses full-length BcRF1 3L_A with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertBcRF1 (BcRF1 EBV (HHV-4))
UseTagsStrepExpressionMammalianMutationAmino acids L64-L66 mutated to alaninePromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF24 3L_A-2xStrep
Plasmid#138440PurposeExpresses full-length ORF24 3L_A with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsStrepExpressionMammalianMutationAmino acids L73-L75 mutated to alaninePromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF24 202-752-3xFLAG
Plasmid#138424PurposeExpresses truncated ORF24 with a C-terminal 3xFLAG tag in mammalian cellsDepositorInsertORF24 202-752 (ORF24 KSHV (HHV-8))
UseTagsFLAGExpressionMammalianMutationAmino acids 202-752PromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF24 3L_A-3xFLAG
Plasmid#138425PurposeExpresses full-length ORF24 3L_A with a C-terminal 3xFLAG tag in mammalian cellsDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsFLAGExpressionMammalianMutationAmino acids L73-L75 mutated to alaninePromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF24 L73A-3xFLAG
Plasmid#138426PurposeExpresses full-length ORF24 L73A with a C-terminal 3xFLAG tag in mammalian cellsDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsFLAGExpressionMammalianMutationAmino acid L73 mutated to alaninePromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF24 L74A-3xFLAG
Plasmid#138427PurposeExpresses full-length ORF24 L74A with a C-terminal 3xFLAG tag in mammalian cellsDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsFLAGExpressionMammalianMutationAmino acid L74 mutated to alaninePromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF24 L75A-3xFLAG
Plasmid#138428PurposeExpresses full-length ORF24 L75A with a C-terminal 3xFLAG tag in mammalian cellsDepositorInsertORF24 (ORF24 KSHV (HHV-8))
UseTagsFLAGExpressionMammalianMutationAmino acid L75 mutated to alaninePromoterCMVAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFPC1-Cerulean3-Cerulean3-FLARE-D1ER
Plasmid#123354PurposeCyan-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator, targeted to endoplasmic reticulum lumen.DepositorInsertcp-moxCerulean3-moxCerulean3-FLARE-D1ER
UseTagsC-terminal ER retention signal (KDEL), cp-moxCeru…ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPK-352
Plasmid#157922PurposepcDNA-CMV-PCYA-IRES-HO1-P2A-FD-P2A-FNRDepositorInsertstPcyA
IRES
HO1
FD
FNR
UseSynthetic Biology; OptogeneticsTagsMitochondrial Targeting Sequence, Mitochondrial T…ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMinDis.X513-iNicSnFR3a-(CC93)-ER
Plasmid#125121PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotineDepositorInsertiNicSnFR3a-(CC93)-ER
UseTagsER retention motifExpressionMammalianMutationPromoterT7Available sinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMinDis.X513-iNicSnFR3b-(V7)-ER
Plasmid#125123PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotineDepositorInsertiNicSnFR3a-(V7)-ER
UseTagsFusion protein -Endoplasmic retention motifExpressionMammalianMutationPromoterT7Available sinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHHM.X513-iNicSnFR3a-(CC93)
Plasmid#124880PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotineDepositorInsertiNicSnFR3a-(CC93)
UseTagsHA Tag and His TagExpressionBacterialMutationNAPromoterT7Available sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHHM.X513-iNicSnFR2-(CC90)
Plasmid#124836PurposeDevelop a family of genetically encoded fluorescent biosensors for nicotine, termed iNicSnFRs thus enabling optical subcellular pharmacokinetics for nicotineDepositorInsertiNicSnFR2-(CC90)
UseTagsHA tags and His TagExpressionBacterialMutationNAPromoterT7Available sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCHKU17.1-2.2
Plasmid#115530PurposeExpression of a fungal biosynthetic gene clusterDepositorInsertS_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLG1-puro-sgATL1-2
Plasmid#109006PurposeCRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)DepositorInsertATL1-sgRNA #2 (ATL1 Human)
UseLentiviralTagsExpressionMammalianMutationPromotermU6 promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCHSU70.1-2.2
Plasmid#110682PurposeExpression of a fungal biosynthetic gene clusterDepositorInsertPKS
UseTagsflagExpressionYeastMutationPromoterAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCHSU70.2-2.2
Plasmid#110683PurposeExpression of a fungal biosynthetic gene clusterDepositorInsertS_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pONL-C1_fibrillarin
Plasmid#65710PurposeExpresses ONL-fibrillarin in mammalian cellsDepositorInsertfibrillarin (FBL Human)
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYNL-C1_fibrillarin
Plasmid#65708PurposeExpresses YNL-fibrillarin in mammalian cellsDepositorInsertfibrillarin (FBL Human)
UseLuciferaseTagsYNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast HA-POLE2
Plasmid#160809PurposeExpress HA-tagged POLE2DepositorInsertHA-POLE2 (POLE2 Human)
UseRetroviralTagsHAExpressionMutationPromoterAvailable sinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
TetON-shYAP
Plasmid#115667PurposeInducible YAP knockdownDepositorInsertYAP shRNA (YAP1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
ATG-2460
Plasmid#108713PurposeEF1-CBR2opt-T2A-copGFP (construct used for lentiviral transduction)DepositorInsertCBR2opt-copGFP fusion
UseLentiviralTagsCBR2opt fused to copGFPExpressionMutationPromega used directed evolution to create CBR (Cl…Promoterelongation factor-1Available sinceOct. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-Venus-Fluc (puro)
Plasmid#140328PurposeLentivirus for expression of Venus-Fluc (firefly luciferase) fusion proteinDepositorInsertVenus-Firefly Luciferase (luc2)
UseLentiviralTagsExpressionMutationPromoterhuman PGK promoterAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-GRET
Plasmid#158222PurposeControl lentiviral plasmid encoding GFP-Nluc BRET reporter from Schaub et al., Cancer Research, 2015.DepositorInsertGFP-Nluc
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
ATG-3925 (CBG2)
Plasmid#167839PurposeExpresses CBG2 (Click Beetle Green 2) in E. coli and mammalian cellsDepositorInsertClick Beetle Green 2
UseTags6-HisExpressionBacterial and MammalianMutationQ348H. Click Beetle Green 2 (CBR2) has 3 amino a…PromoterCMV, T7Available sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCherryC1 VAP-A
Plasmid#226407PurposeExpression of human VAP-A fused to mCherry in mammalian cellsDepositorInsertVAP-A (VAPA Human)
UseTagsT7-Xpress tag and mCherryExpressionMammalianMutationPromoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
Rtn4a-GFP
Plasmid#61807PurposeHuman Rtn4a fluorescently tagged with GFP on the C-terminus for mammalian expressionDepositorInsertRtn4a (RTN4 Human)
UseTagsAcGFPExpressionMammalianMutationRtn4a Stop Codon removedPromoterCMVAvailable sinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
CHOP promoter (-649/+136) pmCherry-1
Plasmid#36035DepositorInsertCHOP promoter (-647 to +136) (DDIT3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 7, 2012AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Mito-7
Plasmid#54160PurposeLocalization: Mitochondria, Excitation: 487, Emission: 509DepositorInsertMito (COX8A Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28a-CohIII-CBM-HIS-ybbR
Plasmid#60866PurposeCharacteristic fingerprint molecule fused to cohesin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced ybbR tagDepositorInsertsCohIII
CBM
UseTags6xHIS, HRV 3C, and ybbRExpressionBacterialMutationCysteine 63 to Serine in CBM modulePromoterT7Available sinceNov. 26, 2014AvailabilityAcademic Institutions and Nonprofits only