We narrowed to 7,705 results for: ALP

1. HA-HIF2alpha-P405A/P531A-pBabe-puro

   Plasmid

   #19006

   Depositor

   William Kaelin

   Article

   Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

   Insert

   Hypoxia inducible factor 2 alpha (EPAS1 Human)
   

   Use

   Retroviral

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   cDNA changed amino acids: P405A and P531A

   Available Since

   Aug. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-EF1alpha-dCas9-KRAB-MeCP2_Hygro

   Plasmid

   #192664

   Purpose

   3rd generation lenti vector encoding dCas9-KRAB-MeCP2 with 2A Hygro resistance marker

   Depositor

   Jeffrey Miner

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A, D839A, H840A and N863A in Cas9

   Promoter

   EF1a

   Available Since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPICZ-pre-pro-alphaf(I)-E2-Crimson

   Plasmid

   #117660

   Purpose

   Test intracellular localisation of E2-Crimson and study the secretion efficiency

   Depositor

   Pau Ferrer, Benjamin Glick

   Article

   Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

   Insert

   pre-pro-alphaf(I)-E2-Crimson
   

   Expression

   Yeast

   Mutation

   This plasmid encodes the fluorescent protein E2-C…

   Promoter

   pAOX1

   Available Since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. PGC-1 alpha promoter 2kb luciferase

   Plasmid

   #8887

   Depositor

   Bruce Spiegelman

   Article

   Handschin et al Proc Natl Acad Sci U S A. 2003 Jun 10. 1

   Insert

   PGC-1 alpha promoter (Ppargc1a Mouse)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Available Since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPICZ-pre-Ost1-alphaf(I)-Btl2

   Plasmid

   #117667

   Purpose

   Study secretion efficiency of Btl2

   Depositor

   Pau Ferrer, Benjamin Glick

   Article

   Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

   Insert

   pre-Ost1-alphaf(I)-Btl2
   

   Expression

   Yeast

   Mutation

   This plasmid encodes the lipase Btl2 together wit…

   Promoter

   pAOX1

   Available Since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. Stat1 alpha Flag pRc/CMV

   Plasmid

   #8691

   Depositor

   Jim Darnell

   Article

   Horvath et al Genes Dev 1995 Apr 15;9(8):984-94.

   Insert

   STAT1 alpha (STAT1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Feb. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSV human p85 alpha (HA tag)

   Plasmid

   #11499

   Depositor

   Ronald Kahn

   Article

   Ueki et al J Biol Chem. 2003 Nov 28. 278(48):48453-

   Insert

   p85 alpha (PIK3R1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. Lenti-EF1alpha-VP64-dCas9-p300_Blast

   Plasmid

   #192654

   Purpose

   3rd generation lenti vector encoding VP64-dCas9-p300 with 2A Blast resistance marker

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   VP64-dCas9-p300
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A; H840A in Cas9

   Promoter

   EF1a

   Available Since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Lenti-EF1alpha-VP64-dCas9-VP64_Blast

   Plasmid

   #192650

   Purpose

   3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A Blast resistance marker

   Depositor

   Jeffrey Miner

   Article

   Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

   Insert

   VP64-dCas9-VP64
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9

   Promoter

   EF1a

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA3-mouse PKA-RIalpha-mEGFP

    Plasmid

    #45525

    Depositor

    Haining Zhong

    Article

    Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

    Insert

    PKA regulatory subunit RI alpha tagged by mEGFP
    

    Tags

    His

    Expression

    Mammalian

    Available Since

    June 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. LV_EF1a_Ikkalpha(S176E,S180E)-P2A-Hygro_Barcode

    Plasmid

    #170236

    Purpose

    Barcoded lentiviral vector to express Ikkalpha (S176E, S180E) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    CHUK (CHUK Human)
    

    Use

    Lentiviral

    Tags

    V5

    Mutation

    S176E, S180E

    Promoter

    EF1a

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-anti-TNFalpha(Ozoralizumab) PAGER(FL)

    Plasmid

    #230024

    Purpose

    Expresses anti-TNFalpha PAGER(FL) in mammalian cells

    Depositor

    Alice Ting

    Article

    Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

    Insert

    pAAV-MT3-Ozoralizumab-TEVcs-ALFA-hM4DxcpGFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. XE107 XPKC alpha-GFP-CS2+

    Plasmid

    #16743

    Depositor

    Randall Moon

    Article

    Sheldahl et al Curr Biol. 1999 Jul 1;9(13):695-8. doi:

    Insert

    PKC-alpha (prkca Frog)
    

    Use

    Xenopus expression

    Tags

    GFP

    Available Since

    March 14, 2008

    Availability

    Academic Institutions and Nonprofits only

14. PCDNA3 Flag p38 alpha (agf)

    Plasmid

    #20352

    Depositor

    Roger Davis

    Article

    Enslen et al J Biol Chem. 1998 Jan 16. 273(3):1741-8.

    Insert

    pDCNA3 Flag p38 alpha (agf) (Mapk14 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Replace Thr 180 and Tyr 182 with Ala and Phe resp…

    Available Since

    April 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA3-mouse PKA-RIIalpha-mEGFP

    Plasmid

    #45527

    Depositor

    Haining Zhong

    Article

    Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

    Insert

    PKA regulatory subunit RII alpha tagged by mEGFP
    

    Tags

    His

    Expression

    Mammalian

    Available Since

    June 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-mouse PKA-Calpha-mEGFP

    Plasmid

    #45521

    Depositor

    Haining Zhong

    Article

    Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

    Insert

    PKA catalytic subunit alpha tagged by mEGFP
    

    Expression

    Mammalian

    Available Since

    June 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. Flag-neo-PP1-Alpha-H248K

    Plasmid

    #171268

    Purpose

    Mammalian expression of 3x-Flag-tagged human PP1 alpha catalytic subunit H248K mutant that loses enzymatic activity

    Depositor

    Steven Balk

    Article

    Liu et al Oncotarget. 2016 Jan 12;7(2):1754-64. do

    Insert

    3x-Flag-tagged human PP1 alpha catalytic subunit H248K mutant (PPP1CA Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    PPP1CA-His248Lys (H248K)

    Promoter

    CMV

    Available Since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDGB3 alpha2 P35S:dCas9:TV:Tnos (GB2047)

    Plasmid

    #160626

    Purpose

    TU for the constitutive expression of dCas9 fused to TV (TALx6 - VP128) activation domains

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    P35s-dCas9:TV-Tnos
    

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDGB3 alpha2 P35S:dCas9:SRDX:Tnos (GB1188)

    Plasmid

    #160608

    Purpose

    TU for the expression of the dCas9 with the repressor domain SRDX as a CT fusion (CRISPR tools)

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

    Insert

    P35s:dCas9:SRDX:tNOS
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. Anti-GABA(A)R, Alpha1 [N95/35R]

    Plasmid

    #177574

    Purpose

    Mammalian Expression Plasmid of anti-GABA(A)R, Alpha1 (Human). Derived from hybridoma N95/35.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-GABA(A)R, Alpha1 (Homo sapiens) recombinant mouse monoclonal antibody (GABRA1 Mouse)
    

    Expression

    Mammalian

    Promoter

    Dual CMV

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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