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Addgene

We narrowed to 2,968 results for: FAS

Showing: 921 - 940 of 2968 results
  1. pBlue-hFX(T133A)-HA

    Plasmid
    #14989
    Depositor
    Insert
    human frataxin cDNA (FXN Human)
    Use
    Tags
    AU1 and HA
    Expression
    Bacterial
    Mutation
    changed threonine 133 to alanine
    Promoter
    Available since
    Aug. 17, 2007
    Availability
    Academic Institutions and Nonprofits only
  2. pBlue-hFX(T133E)-HA

    Plasmid
    #14990
    Depositor
    Insert
    human frataxin cDNA (FXN Human)
    Use
    Tags
    AU1 and HA
    Expression
    Bacterial
    Mutation
    changed threonine 133 to glutamic acid
    Promoter
    Available since
    Aug. 17, 2007
    Availability
    Academic Institutions and Nonprofits only
  3. pBlue-hFX-HA(AscI)

    Plasmid
    #15404
    Depositor
    Insert
    human frataxin cDNA (FXN Human)
    Use
    Tags
    AU1 and HA
    Expression
    Bacterial
    Mutation
    700 bp insert can be released by digestion with r…
    Promoter
    Available since
    Aug. 17, 2007
    Availability
    Academic Institutions and Nonprofits only
  4. CIRTS-control

    Plasmid
    #213788
    Purpose
    CIRTS RNA targeting system for targeted knockdown in neurons. CIRTS-Calm3 and scramble control guide RNA is expressed under human Syn1 and U6 promoter, respectively.
    Depositor
    Insert
    CIRTS-Calm3-U6-control gRNA
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Syn1, U6
    Available since
    Feb. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. CIRTS-Gas5

    Plasmid
    #213172
    Purpose
    CIRTS RNA targeting system for targeted knockdown in neurons. CIRTS-Calm3 and Gas5 guide RNA is expressed under human Syn1 and U6 promoter, respectively.
    Depositor
    Insert
    CIRTS-Calm3-U6-Gas5 gRNA
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Syn1, U6
    Available since
    Feb. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. pmEos2-N1 GBP (GBP-mEos2)

    Plasmid
    #162876
    Purpose
    Expression in mammalian cells of GFP binding protein (GBP) tagged with photoconvertable protein mEos2 to track GFP proteins of interest to perform Fluorescent intrabody Localization Microscopy
    Depositor
    Insert
    GFP Binding Protein tagged with mEos2
    Use
    Tags
    mEos2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
  7. pHybr_r2a>mG2b-srt-his

    Plasmid
    #124804
    Purpose
    HDR template to knock-in mouse IgG2b Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG2b producing cell lines.
    Insert
    Murine IgG2b
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
  8. pHybr_r2a>mG2a-srt-his

    Plasmid
    #124803
    Purpose
    HDR template to knock-in mouse IgG2a Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG2a producing cell lines.
    Insert
    Murine IgG2a
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
  9. PX459-gR2A_Hinge

    Plasmid
    #124811
    Purpose
    Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a>Fab-srt-his plasmids to convert expression of hybridomas to Fab' fragments.
    Insert
    Cas9 – gRNA_R2A_hinge
    Use
    CRISPR
    Tags
    3XFLAG and GFP
    Expression
    Bacterial and Mammalian
    Mutation
    R166H in PuroR (please see depositors comment bel…
    Promoter
    pUC
    Available since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
  10. pHybr_r2a>mG1-srt-his

    Plasmid
    #124802
    Purpose
    HDR template to knock-in mouse IgG1 Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG1 producing cell lines.
    Insert
    Murine IgG1
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    -
    Available since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
  11. pHybr_r2a>mA-srt-his

    Plasmid
    #124806
    Purpose
    HDR template to knock-in mouse IgA Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgA producing cell lines.
    Insert
    Murine IgA
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
  12. pHybr_r2a>mG3-srt-his

    Plasmid
    #124805
    Purpose
    HDR template to knock-in mouse IgG3 Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG3 producing cell lines.
    Insert
    Murine IgG3
    Use
    CRISPR; Hdr template
    Tags
    Histag (HHHHHH) and Sortag (LPETGG)
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Sept. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
  13. pBlue-hFX-HA(NcoI/EcoRI)

    Plasmid
    #15405
    Depositor
    Insert
    human frataxin cDNA (FXN Human)
    Use
    Tags
    AU1 and HA
    Expression
    Bacterial
    Mutation
    insert can be released by digestion with REs NcoI…
    Promoter
    Available since
    Aug. 17, 2007
    Availability
    Academic Institutions and Nonprofits only
  14. pGL3-basic-hFX-promotor(2kb, distal)

    Plasmid
    #14981
    Depositor
    Insert
    human frataxin promoter fragment (FXN Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    includes distal fragment of human frataxin promot…
    Promoter
    Available since
    Aug. 17, 2007
    Availability
    Academic Institutions and Nonprofits only
  15. pBlue-hFX(I154F)-HA

    Plasmid
    #14988
    Depositor
    Insert
    human frataxin cDNA (FXN Human)
    Use
    Tags
    AU1 and HA
    Expression
    Bacterial
    Mutation
    changed isoleucine 154 to phenylalanine
    Promoter
    Available since
    Aug. 17, 2007
    Availability
    Academic Institutions and Nonprofits only
  16. pBlue-hFX(T133A)-HA

    Plasmid
    #14989
    Depositor
    Insert
    human frataxin cDNA (FXN Human)
    Use
    Tags
    AU1 and HA
    Expression
    Bacterial
    Mutation
    changed threonine 133 to alanine
    Promoter
    Available since
    Aug. 17, 2007
    Availability
    Academic Institutions and Nonprofits only
Showing: 921 - 940 of 2968 results