We narrowed to 19,918 results for: TIM
-
Plasmid#60152PurposeFirefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by LEU1 promoter.DepositorInsertFLuc-yEVenus-PEST
TagsPEST degronExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterLEU1prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB789
Plasmid#60166PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by RNR1 promoter.DepositorInsertFLuc-yEVenus-PEST
TagsPEST degronExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterRNR1prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB682
Plasmid#60143PurposeFirefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by MET17 promoter.DepositorInsertFLuc-yEVenus-PEST
TagsPEST degronExpressionYeastMutationA4VPromoterMET17prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
TALE Toolbox
Plasmid Kit#1000000019PurposeA complete set of plasmids, optimized for human expression, to build your own TALE nucleases (TALENs) and transcription factors (TALE-TFs) for genome editing and transcription modulation applications.DepositorApplicationGenome EditingVector TypeMammalian ExpressionEditing TypeTALENAvailable SinceDec. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_ACh3.0 (AAV9)
Viral Prep#121922-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSyn-GRAB_ACh3.0 (#121922). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_ACh3.0 plasmid DNA. Syn-driven expression of the genetically-encoded fluorescent acethycholine(ACh) sensor GRAB-ACh3.0. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA (AAV1)
Viral Prep#68717-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA (#68717). In addition to the viral particles, you will also receive purified pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA plasmid DNA. CAG-driven, Cre dependent, GCaMP6s calcium sensor and bicistronic, physically separate mRuby2 expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAG-FLEXTagsmRuby2 (Cre-dependent)Available SinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST (AAV9)
Viral Prep#109048-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST (#109048). In addition to the viral particles, you will also receive purified pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST plasmid DNA. Cre-dependent expression of the anion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites, translated from an internal ribosomal entry site (IRES) following mRuby3 expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsmRuby3Available SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA (AAV1)
Viral Prep#68719-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA (#68719). In addition to the viral particles, you will also receive purified pAAV-CAG-Flex-mRuby2-GSG-P2A-GCaMP6f-WPRE-pA plasmid DNA. CAG-driven, Cre-dependent GCaMP6f calcium sensor with bicistronic mRuby2. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAG-FLEXTagsmRuby2 (Cre-dependent)Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-FLEX-jYCaMP1s (AAV1)
Viral Prep#135420-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-syn-FLEX-jYCaMP1s (#135420). In addition to the viral particles, you will also receive purified pAAV-syn-FLEX-jYCaMP1s plasmid DNA. Synapsin-driven, Cre-dependent expression of calcium sensor YCaMP1s. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable SinceJuly 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-syn-FLEX-axon-jYCaMP1s (AAV1)
Viral Prep#135421-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-syn-FLEX-axon-jYCaMP1s (#135421). In addition to the viral particles, you will also receive purified pAAV-syn-FLEX-axon-jYCaMP1s plasmid DNA. Syn-driven, Cre-dependent axon-targeted YCaMP1s calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRiboI-Sec-APEX2m
Plasmid#176845PurposeExpression of APEX2 in the periplasm from a codon-optimized gene (single-copy integrating plasmid)DepositorInsertpRibo-Sec-APEX2m
ExpressionBacterialPromoterpRibo, theophilline riboswitch inducible hsp60 pr…Available SinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLT 653
Plasmid#59996PurposeExpresses a hybrid dsRNA corresponding to the Caenorhabditis elegans dhc-3/klp-16 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progenyDepositorExpressionBacterialMutationpartial genomicAvailable SinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRibo-Sec-APEX2m
Plasmid#176844PurposeExpression of APEX2 in the periplasm from a codon-optimized gene (multi-copy episomal plasmid)DepositorInsertpRibo-Sec-APEX2m
UseInducibleExpressionBacterialPromoterpRibo, theophilline riboswitch inducible hsp60 pr…Available SinceJan. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV-opEBNA2-IRES-mCherry
Plasmid#220355PurposeExpresses EBV latent gene EBNA2 in mammalian cells (EBNA2 coding sequence was optimised for expression)DepositorInsertopEBNA2 (EBNA-2 Epstein-Barr Virus (EBV) strain B95-8)
UseRetroviralExpressionMammalianMutationcodon-optimised for expression in mammalian cellsAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLT 651
Plasmid#59998PurposeExpresses a hybrid dsRNA corresponding to the Caenorhabditis elegans klp-16/him-8 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progenyDepositorExpressionBacterialMutationpartial genomicAvailable SinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLT 652
Plasmid#59997PurposeExpresses a hybrid dsRNA corresponding to the Caenorhabditis elegans dhc-3/him-8 genes in bacteria; ingestion of the bacteria by C elegans produces an RNAi response & leads to more male progenyDepositorAvailable SinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-V5.hOr47a
Plasmid#126474PurposeHuman codon-optimized version of D. melanogaster Or47a (hOr47a) without N-terminal tags for improved intracellular trafficking in mammalian cellsDepositorInsertOr47a (Or47a Fly)
TagsV5 tagExpressionMammalianMutationcodon optimization for H. sapiensPromoterCMVAvailable SinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA (AAV8)
Viral Prep#154867-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA (#154867). In addition to the viral particles, you will also receive purified pAAV-hSyn-fDIO-hM4D(Gi)-mCherry-WPREpA plasmid DNA. Syn-driven, Flp-dependent hM4D(Gi) receptor with an mCherry reporter for CNO-induced neuronal inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPromoterHuman Synapsin-1TagsmCherry (Flp-dependent)Available SinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
Human sgRNA library Brunello in lentiGuide-Puro (Lentiviral Prep)
Viral Prep#73178-LVPurposeReady-to-use Lentiviral Prep particles produced from Human sgRNA library Brunello in lentiGuide-Puro (#73178). In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiGuide-Puro plasmid DNA. Ready-to-use lentiviral pooled library for CRISPR screening in human cells. Use on cells that are stably expressing Cas9 to make edits across 19,114 genes in the human genome.DepositorAvailable SinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Mouse sgRNA library Brie in lentiGuide-Puro (Lentiviral Prep)
Viral Prep#73633-LVPurposeReady-to-use Lentiviral Prep particles produced from Mouse sgRNA library Brie in lentiGuide-Puro (#73633). In addition to the viral particles, you will also receive purified Mouse sgRNA library Brie in lentiGuide-Puro plasmid DNA. <p><p>Ready-to-use lentiviral pooled library for CRISPR screening in mouse cells. Use on cells that are stably expressing Cas9 to make edits across 19,674 genes in the mouse genome.</p></p>DepositorAvailable SinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only