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Showing: 921 - 940 of 2166 results

  1. pTPH405c

    Plasmid
    #185726
    Purpose
    Directed evolution of Cas9 adenine base editors with altered PAM compatibility
    Depositor
    David Liu
    Article
    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038
    Insert
    gIII(N)-NpuN-NpuC-gIII(C) single PAM linker
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    185726-plasmid-map-sequence-id-365709

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  10. pĪ±H-S-GSAS-2P-OMICRON

    Plasmid
    #180593
    Purpose
    Mammalian cell expression of SARS-CoV-2 Spike protein variant OMICRON with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987
    Depositor
    Priyamvada Acharya
    Article
    Gobeil et al Mol Cell. 2022 Jun 2;82(11):2050-2068.e6
    Insert
    Spike (S-GSAS-2P-OMICRON) (S Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    HRV3C cleavage site, 8X His tag, 2X Strep-Tag II
    Expression
    Mammalian
    Mutation
    Ectodomain (AAs 1-1208); 682-685 (furin site = RRā€¦
    Promoter
    Available since
    Jan. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180593-plasmid-map-sequence-id-352871

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  15. pYPQ262m3

    Plasmid
    #149556
    Purpose
    Gateway entry clone for CRISPR-iSpyMacCas9 ABE7.10 A-G base editing at NAAR PAMs
    Depositor
    Yiping Qi
    Article
    Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d
    Insert
    ecTadAwt-ecTadAmut-ziSpyMacCas9(D10A)
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    D10A, R221K, N394K, Protospacer interacting domaiā€¦
    Promoter
    Available since
    July 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    149556-plasmid-map-sequence-id-306141

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  17. pAM57

    Plasmid
    #227654
    Purpose
    pETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, V80A)
    Depositor
    Andreas Martin
    Article
    Worden et al Nat Struct Mol Biol. 2014 Mar;21(3):220-
    Inserts
    Rpn11 (RPN11 Budding Yeast)
    Rpn8 (RPN8 Budding Yeast)
    Use
    Tags
    H6-PreScission and PreScission-Strep
    Expression
    Bacterial
    Mutation
    1-179 and amino acids 2-239, V80A
    Promoter
    T7
    Available since
    Dec. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    227654-plasmid-map-sequence-id-455272

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  18. pAM54

    Plasmid
    #227653
    Purpose
    pETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, L132A)
    Depositor
    Andreas Martin
    Article
    Worden et al Nat Struct Mol Biol. 2014 Mar;21(3):220-
    Inserts
    Rpn11 (RPN11 Budding Yeast)
    Rpn8 (RPN8 Budding Yeast)
    Use
    Tags
    H6-PreScission and PreScission-Strep
    Expression
    Bacterial
    Mutation
    1-179 and amino acids 2-239, L132A
    Promoter
    T7
    Available since
    Dec. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    227653-plasmid-map-sequence-id-455251

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Showing: 921 - 940 of 2166 results