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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 5,553 results for: golden gate

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Showing: 921 - 940 of 5553 results

  1. C40m

    Plasmid
    #120973
    Purpose
    MoClo golden gate assembly CD part for blank part (random 50bp sequence) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    Blank (Random 50bp sequence)
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120973-plasmid-map-sequence-id-236027

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  2. C20m

    Plasmid
    #120961
    Purpose
    MoClo golden gate assembly CD part with sfYFP-ssrA (superfolder YFP with ssrA degradation tag; variant of https://www.fpbase.org/protein/yfp/)
    Depositor
    Richard Murray
    Insert
    sfYFP-ssrA (has BbsI site)
    Use
    Synthetic Biology
    Tags
    ssrA degradation tag
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120961-plasmid-map-sequence-id-235979

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  3. pAV10.KN

    Plasmid
    #63209
    Purpose
    pCACS backbone (Amp), cloNAT, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI).
    Depositor
    Jef Boeke
    Publication
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available since
    April 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    63209-plasmid-map-sequence-id-115799

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  4. C24m

    Plasmid
    #120964
    Purpose
    MoClo golden gate assembly CD part for lambda cI (repressor of pL or pOR1-OR2; use C64m for temperature-sensitive version). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    lambda-cI
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120964-plasmid-map-sequence-id-236100

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  5. C91m

    Plasmid
    #120999
    Purpose
    MoClo golden gate assembly CD part for sfCFP (superfolder CFP developed by Ye Chen in Matthew Bennet's lab) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    sfCFP (Chen, Bennett)
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120999-plasmid-map-sequence-id-236129

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  6. C3m

    Plasmid
    #120956
    Purpose
    MoClo golden gate assembly CD part for sfGFP (Superfolder green fluorescent protein from DOI: 10.1038/nbt1172; slight variant on https://www.fpbase.org/protein/superfolder-gfp/)
    Depositor
    Richard Murray
    Insert
    sfGFP
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120956-plasmid-map-sequence-id-235970

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  7. C27m

    Plasmid
    #120965
    Purpose
    MoClo golden gate assembly CD part for TetR (tet repressor; identical to C2d but without ssrA degradation tag). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    tetR (no deg tags)
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120965-plasmid-map-sequence-id-236387

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  8. C31m

    Plasmid
    #120966
    Purpose
    MoClo golden gate assembly CD part for Bxb1 integrase. See DOI: 10.1007/s00438-006-0129-5. Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    bxb1 integrase
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120966-plasmid-map-sequence-id-236888

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  9. Rich-pT7GG

    Plasmid
    #108443
    Purpose
    This plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pT7TS-rich. The vector is compatible for Golden-gate assembly
    Depositor
    Richard Bélanger
    Type
    Empty backbone
    Use
    Xenopus oocyte expression system
    Promoter
    T7
    Available since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    108443-plasmid-map-sequence-id-213670

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  10. pUC-GW-Kan-L0-TaSEP1B6(int)

    Plasmid
    #242006
    Purpose
    L0 CDS1 Golden Gate part of the pre-mRNA (excluding UTRs) of Triticum aestivum SEPALLATA1-6 (SEP1-6) B-subgenome homoeolog (TraesCS5B02G396700)
    Depositor
    Cristobal Uauy
    Insert
    TaSEP1-B6_with_introns
    Use
    Synthetic Biology
    Expression
    Plant
    Available since
    March 30, 2026
    Availability
    Academic Institutions and Nonprofits only
    242006-plasmid-map-sequence-id-504502

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  11. V35m

    Plasmid
    #121042
    Purpose
    MoClo golden gate assembly AE destination vector DVCK-AE-GFP (ColE1/kan resistance destination vector with constitutive GFP expression for screening.
    Depositor
    Richard Murray
    Insert
    Kan destination vector with ColE1 origin and GFP selection
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121042-plasmid-map-sequence-id-247704

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  12. T1m

    Plasmid
    #121028
    Purpose
    MoClo golden gate assembly DE part for T500 terminator Engineered terminator derived from bacteriophage 83; see DOI: 10.1126/science.284.5414.611).
    Depositor
    Richard Murray
    Insert
    T500_noGap, short attachment (T11)
    Use
    Synthetic Biology
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121028-plasmid-map-sequence-id-245901

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  13. pAV11K

    Plasmid
    #63184
    Purpose
    pRS416 backbone, URA3 replaced by KanMX, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.
    Depositor
    Jef Boeke
    Publication
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    63184-plasmid-map-sequence-id-104886

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  19. p028g34-3Cs

    Plasmid
    #249636
    Purpose
    Clone the cleavage site for 3C protease between the N-terminal tag and the CDS of the protein to be expressed using pET28g backbone, using fusion sites 3 and 4
    Depositor
    Isabel Abreu
    Publication
    Luis et al PLoS One. 2025 Jul 7;20(7):e0327341. doi
    Insert
    Recognition site for 3C protease
    Expression
    Bacterial
    Available since
    April 10, 2026
    Availability
    Academic Institutions and Nonprofits only
    249636-plasmid-map-sequence-id-506116

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  20. p028g34-TEVs

    Plasmid
    #249633
    Purpose
    Clone the cleavage site for TEV protease between the N-terminal tag and the CDS of the protein to be expressed using pET28g backbone, using fusion sites 3 and 4
    Depositor
    Isabel Abreu
    Publication
    Luis et al PLoS One. 2025 Jul 7;20(7):e0327341. doi
    Insert
    Recognition site for TEV protease
    Expression
    Bacterial
    Available since
    April 10, 2026
    Availability
    Academic Institutions and Nonprofits only
    249633-plasmid-map-sequence-id-506112

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Showing: 921 - 940 of 5553 results