We narrowed to 48,380 results for: Ide

1. pgRNA-modified

   Plasmid

   #84477

   Purpose

   guide RNA expression lentiviral construct

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

   Insert

   guide RNA
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   mU6

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCold CL7-Cas9

   Plasmid

   #124890

   Purpose

   obtaining full Cas9 RNP

   Depositor

   Yi Liu

   Article

   Qiao et al Commun Biol. 2019 May 3;2:161. doi: 10.1

   Insert

   Cas9
   

   Expression

   Bacterial

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMJ806

   Plasmid

   #39312

   Depositor

   Jennifer Doudna

   Article

   Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   His6 and MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 7, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSEED-sfGFP

   Plasmid

   #219639

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   sfGFP SEED Cassette
   

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. RhoG

   Plasmid

   #55694

   Purpose

   expression of mouse RhoG

   Depositor

   Julian Downward

   Article

   Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

   Insert

   RhoG (Rhog Mouse)
   

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   Sept. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pBZS-YP_009702000

   Plasmid

   #198379

   Purpose

   Express YP_009702000 in bacterial cells.

   Depositor

   Takashi Ito

   Article

   Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

   Insert

   YP_009702000 (CVA-1_913R )
   

   Expression

   Bacterial

   Available Since

   April 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. TLCV2-ApaLI-FLAG-T2A-mTagBFP2-Puro

   Plasmid

   #211462

   Purpose

   Lentivirus backbone expressing a doxycycline-inducible mitochondrial localized ApaLI

   Depositor

   Agnel Sfeir

   Article

   Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

   Insert

   ApaLI
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLKO-RFP-shCntrl

   Plasmid

   #69040

   Purpose

   Lentiviral expression of control shRNA

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   control shRNA
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEF6a-CD28-PafA

   Plasmid

   #113400

   Purpose

   Expresses CD28-PafA in mammalian cell

   Depositor

   Min Zhuang

   Article

   Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

   Insert

   CD28-PafA
   

   Tags

   myc

   Expression

   Mammalian

   Promoter

   EF-1α

   Available Since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHH0103_YWHAQ_14-3-3

    Plasmid

    #109943

    Purpose

    Protein expression and purification of YWHAQ_14-3-3

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    YWHAQ_14-3-3
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSQT1313

    Plasmid

    #53370

    Purpose

    multiplex gRNA expression plasmid

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    None
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. psd44-GqQL

    Plasmid

    #46826

    Purpose

    2nd generation lentiviral vector for expression of mutant G protein alpha q in mammalian cells

    Depositor

    Agnese Mariotti

    Insert

    g protein alpha q (GNAQ Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Q209L (The mutation reduces GTPase activity resul…

    Promoter

    PGK promoter

    Available Since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pC008 - pBR322 with tetR-inducible RFP

    Plasmid

    #79157

    Purpose

    inducible RFP expression vector

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

    Insert

    TetR + RFP
    

    Available Since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1 FLII12Pglu-700uDelta6

    Plasmid

    #17866

    Depositor

    Wolf Frommer

    Article

    Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

    Insert

    FLII12Pglu-700uDelta6
    

    Tags

    Citrine

    Expression

    Mammalian

    Mutation

    700uDelta6, ECFP insert in mglB

    Available Since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. GST-RNF126

    Plasmid

    #138643

    Purpose

    Bacterial expression of GST-RNF126 fusion protein

    Depositor

    Jane McGlade

    Article

    Smith et al J Cell Sci. 2013 Mar 15;126(Pt 6):1366-8

    Insert

    RNF126 (RNF126 Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. p3258 pCMV hUBC9 wt HA

    Plasmid

    #14438

    Depositor

    Peter Howley

    Article

    Yasugi et al Nucleic Acids Res. 1996 Jun 1. 24(11):20

    Insert

    UBC9 (UBE2I Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMSSM110: pCMV-HA-NLS-SpuFz1-v2

    Plasmid

    #205277

    Purpose

    Human expression vector for human codon optimized N-terminal NLS-tagged SpuFz1-v2

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1
    

    Tags

    HA, NLS(Makkerh)

    Expression

    Mammalian

    Mutation

    C310R/D487K/T513K

    Available Since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. GLUT1-eGFP/pcDNA-DEST47

    Plasmid

    #18729

    Depositor

    Wolf Frommer

    Article

    Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

    Insert

    pcDNA-DEST47 /hGLUT1-eGFP
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted TAA

    Available Since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. Tol2_8xCTS-ECFP

    Plasmid

    #200249

    Purpose

    Zebrafish transgenics; Plasmid encodes the sgRNA2_8xCTS-ECFP reporter and could be stably integrated in the zebrafish genome using Tol2 transgenesis

    Depositor

    Tatjana Sauka-Spengler

    Article

    Pelea et al bioRxiv 2023.05.08.539738

    Insert

    8xCTS-ECFP
    

    Use

    Zebrafish expression

    Available Since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. +36GFP-LPETG

    Plasmid

    #71754

    Purpose

    +36GFP-LPETG for expression in E.Coli

    Depositor

    David Liu

    Article

    Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

    Insert

    (GGS)9_(pos36GFP)_6XHis_LPETG
    

    Tags

    6XHis and Sortase (LPETG)

    Expression

    Bacterial

    Available Since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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