We narrowed to 7,052 results for: plasmid dna

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pPP089

Plasmid

#158796

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-3 in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-3

Expression

Bacterial

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pPP076

Plasmid

#158794

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-1 in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-1

Expression

Bacterial

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pPP378

Plasmid

#158797

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-1(D371A) in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

dCasPhi-1 (D371A)

Expression

Bacterial

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pPP384

Plasmid

#158799

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-3(D413A) in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

dCasPhi-3 (D413A)

Expression

Bacterial

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pVC-CRISPR1

Plasmid

#120366

Purpose

Plasmid containing shortened version of upstream V-C CRISPR array for integration assays

Depositor

Jennifer Doudna

Article

Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

Insert

CRISPR1

Use

CRISPR

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pVC-CRISPR

Plasmid

#120369

Purpose

Plasmid containing shortened versions of both natural V-C CRISPR arrays for preparing sequencing library

Depositor

Jennifer Doudna

Article

Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

Inserts

CRISPR1
CRISPR2

Use

CRISPR

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pTXB1-nbOcIgG-DddA11_NT

Plasmid

#232441

Purpose

Anti-rabbit IgG Fc specific nanobody-DddA11_NT for D&Dseq (sc TFs molecular footprint)

Depositor

Ivan Raimondi

Article

Chi et al bioRxiv [Preprint]. 2025 Jan 2:2024.12.3

Insert

nbOcIgG-DddA11_N

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pTXB1-nbMmKappa-DddA11_NT

Plasmid

#232442

Purpose

Anti-mouse kappa chain specific nanobody-DddA11_NT for D&Dseq (sc TFs molecular footprint)

Depositor

Ivan Raimondi

Article

Chi et al bioRxiv [Preprint]. 2025 Jan 2:2024.12.3

Insert

nbMmKappa-DddA11_NT

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pKAR1

Plasmid

#23104

Purpose

Allows expression of shRNA from U6 promoter and a rescue cDNA from a doxycycline (Tet) inducible promoter in the same plasmid

Depositor

Randy Poon

Article

Ma et al Nucleic Acids Res. 2007 . 35(4):e22.

Type

Empty backbone

Use

RNAi

Tags

Flag

Expression

Mammalian

Promoter

TRE for cDNA; U6 for shRNA

Available Since

May 27, 2011

Availability

Academic Institutions and Nonprofits only

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SJ358

Bacterial Strain

#67755

Purpose

a derivative of E. coli strain NCM3722 which has been cured of the F plasmid

Depositor

Suckjoon Jun

Article

Brown et al Genome Announc. 2015 Aug 6;3(4). pii: e0

Bacterial Resistance

None

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only