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Flag‐mTim3 (T2 truncation)

Plasmid

#49211

Purpose

expresses Flag tagged mouse Tim3 (T2 truncation)

Depositor

Lawrence Kane

Article

Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

Insert

Tim3 T2 truncation (Havcr2 Mouse)

Tags

FLAG and signal sequence

Expression

Mammalian

Mutation

T2 truncation ( lacks all but one tyrosine, which…

Promoter

EF1a

Available Since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 hSlp4-a C2AB

Plasmid

#40037

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a C2AB (SYTL4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

C2A and C2B domains (353-671)

Promoter

CMV promoter

Available Since

Oct. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 hSlp4-a C2A

Plasmid

#40038

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a C2A (SYTL4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

C2A domain (353-487)

Promoter

CMV promoter

Available Since

Oct. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pSBbi-Pur-mCerulean3-spacer-cpVenus[E172]

Plasmid

#125204

Purpose

SB-transposon plasmid for stable expression of mCerulean3-cpVenus[E172] FRET low control

Depositor

Eric Davis

Article

Havranek et al Blood. 2017 Aug 24;130(8):995-1006. doi:

Insert

mCerulean3-spacer-cpVenus[E172]

Use

Transposon

Expression

Mammalian

Promoter

EF1a/RPBSA

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3 CCR5 HHE Short

Plasmid

#137697

Purpose

Encodes region -2761 to -1814 of CCR5 promoter HHE Haplotype fused to firefly luciferase

Depositor

Anjali Joshi

Article

Joshi et al Sci Rep. 2017 Mar 22;7(1):232. doi: 10.1

Insert

CCR5 Promoter (CCR5 Human)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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Flag‐mTim3 (T1 truncation)

Plasmid

#49210

Purpose

expresses Flag tagged mouse Tim3 (T1 truncation)

Depositor

Lawrence Kane

Article

Lee et al Mol Cell Biol. 2011 Oct;31(19):3963-74.

Insert

Tim3 T1 truncation (Havcr2 Mouse)

Tags

FLAG and signal sequence

Expression

Mammalian

Mutation

T1 truncation ( contains all but the three most C…

Promoter

EF1a

Available Since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pEN_TT MEK5-DD-mRFP1

Plasmid

#47547

Purpose

Gateway entry vector for an inducible mRFP1-tagged constitutively active MEK5 mutant

Depositor

Kevin Janes

Article

Jensen et al Cell Host Microbe. 2013 Jan 16;13(1):67-

Insert

MEK5-DD (MAP2K5 Human)

Use

Entry vector

Tags

mRFP

Mutation

S311D, T315D

Available Since

Sept. 6, 2013

Availability

Academic Institutions and Nonprofits only
pEGFP-C1 hSlp4-a C2B

Plasmid

#40039

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp4-a C2B (SYTL4 Human)

Tags

EGFP

Expression

Mammalian

Mutation

C2B domain (488-671)

Promoter

CMV promoter

Available Since

Oct. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pGEX4T-1 hSlp2-a del.C2AB

Plasmid

#40059

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp2-a del.C2AB (SYTL2 Human)

Tags

GST

Expression

Bacterial

Mutation

deletion of C2A and C2B domains (601-910)

Promoter

Tac

Available Since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only
pGL3 CCR5 HHG Short

Plasmid

#137699

Purpose

Encodes region -2761 to -1814 of CCR5 promoter HHG Haplotype fused to firefly luciferase

Depositor

Anjali Joshi

Article

Joshi et al Sci Rep. 2017 Mar 22;7(1):232. doi: 10.1

Insert

CCR5 Promoter (CCR5 Human)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-C1 hSlp2-a V18A

Plasmid

#40054

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

hSlp2-a V18A (SYTL2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Valine 18 to Alanine

Promoter

CMV promoter

Available Since

Oct. 19, 2012

Availability

Academic Institutions and Nonprofits only
pLKO-tet-puro-sh-ex16b

Plasmid

#35168

Depositor

Benjamin Blencowe

Article

Gabut et al Cell. 2011 Sep 30;147(1):132-46. Epub 20

Insert

Foxp1 (Foxp1 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-C1 Slp2-a C2B

Plasmid

#40053

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

Slp2-a C2B (Sytl2 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

C2B domain

Promoter

CMV promoter

Available Since

Nov. 9, 2012

Availability

Academic Institutions and Nonprofits only
NFATc2-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75233

Purpose

CRISPR/Cas9 plasmid against human NFATc2

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc2 (NFATC2 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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NFATc2-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75234

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc2 (1/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc2 (NFATC2 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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NFATc2-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75235

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc2 (2/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc2 (NFATC2 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX4T-1 Slp2-a E11A/R32A

Plasmid

#40063

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

Slp2-a E11A/R32A (Sytl2 Mouse)

Tags

GST

Expression

Bacterial

Mutation

Glutamate 11 to Alanine, Arginine 32 to Alanine

Promoter

Tac

Available Since

Nov. 9, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-C1 Slp2-a E11A/R32A

Plasmid

#40055

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

Slp2-a E11A/R32A (Sytl2 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Glutamate 11 to Alanine, Arginine 32 to Alanine

Promoter

CMV promoter

Available Since

Nov. 9, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-C1 Slp2-a C2A

Plasmid

#40052

Depositor

Fernando Martin-Belmonte

Article

Galvez-Santisteban et al Nat Cell Biol. 2012 Jul 22. doi: 10.1038

Insert

Slp2-a C2A (Sytl2 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

C2A domains

Promoter

CMV promoter

Available Since

Nov. 9, 2012

Availability

Academic Institutions and Nonprofits only
pEN_TT 3xHA-MKK6-EE

Plasmid

#47544

Purpose

Gateway entry vector for an inducible 3xHA-tagged constitutively active MKK6 mutant

Depositor

Kevin Janes

Article

Jensen et al Cell Host Microbe. 2013 Jan 16;13(1):67-

Insert

3xHA-MKK6-EE (MAP2K6 Human)

Use

Entry vector

Tags

3XHA

Mutation

S207E, T211E

Available Since

Sept. 6, 2013

Availability

Academic Institutions and Nonprofits only