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NmCas9_sgRNA_expression_in_pBluescript

Plasmid

#122091

Purpose

U6 driven NmCas9 sgRNA expression vector for cloning own guides

Depositor

Scot Wolfe

Publication

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

NmCas9 tracrRNA (NEWENTRY S. pyogenes, Synthetic)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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SpCas9_sgRNA_expression_in_pBluescript

Plasmid

#122089

Purpose

U6 driven SpCas9 sgRNA expression vector for cloning own guides

Depositor

Scot Wolfe

Publication

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SpCas9 tracr (NEWENTRY S. pyogenes, Synthetic)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pAM4937 (pCV0049)

Plasmid

#120082

Purpose

Cyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 2 (NS2).

Depositor

Susan Golden

Publication

Taton et al Nucleic Acids Res. 2014 Jul 29. pii: gku

Insert

Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS2, CV-aph1, CV-ccdB-SwaI

Use

Other

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pUC57-4eCOL2A1

Plasmid

#97211

Purpose

For cloning of a COL2A1-based, chondrogenesis-responsive promoter, and subcloning this insert into new vectors

Depositor

Ryan Porter

Publication

De La Vega et al Tissue Eng Part C Methods. 2019 Feb 6. d

Insert

4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)

Use

Vector is designed for cloning and generation of …

Promoter

COL2A1 regulatory elements

Available since

Oct. 13, 2017

Availability

Academic Institutions and Nonprofits only

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Kusabira Orange-N1 (KO)

Plasmid

#54793

Purpose

Localization: N1 Cloning Vector, Excitation: 548, Emission: 561

Depositor

Michael Davidson, Atsushi Miyawaki

Publication

Karasawa et al Biochem J. 2004 Jul 1. 381(Pt 1):307-12.

Type

Empty backbone

Tags

Kusabira Orange

Expression

Mammalian

Available since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pPOM034_P7_3"Lys3

Plasmid

#216459

Purpose

3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Publication

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

3''lys3

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM038_P8b_5'Lys3

Plasmid

#216463

Purpose

5' homology arm for S. pombe genomic integration. Part type 8b following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Publication

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

5'lys3

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM040_P8b_5'Ura4

Plasmid

#216461

Purpose

5' homology arm for S. pombe genomic integration. Part type 8b following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Publication

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

5'ura4

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM036_P7_3"Ura4

Plasmid

#216465

Purpose

3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Publication

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

3''ura4

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM024_P2_pMPA1

Plasmid

#216449

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Publication

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter mpa1

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM022_P2_pRPS1002

Plasmid

#216447

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Publication

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter rps1002

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM020_P2_pTRS1

Plasmid

#216445

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Publication

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter trs1

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM014_P2_pGPM1

Plasmid

#216439

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Publication

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter gpm1

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available since

May 9, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM032_P4_tSynth30

Plasmid

#216457

Purpose

Synthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Publication

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Terminator synth30

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM031_P4_tSynth29

Plasmid

#216456

Purpose

Synthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Publication

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Terminator synth29

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM030_P4_tSynth27

Plasmid

#216455

Purpose

Synthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Publication

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Terminator synth27

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM029_P4_tSynth25

Plasmid

#216454

Purpose

Synthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Publication

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Terminator synth25

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM028_P4_tSynth3

Plasmid

#216453

Purpose

Synthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Publication

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Terminator synth3

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM027_P4_tSynthGuo

Plasmid

#216452

Purpose

Synthetic terminator for gene expression in yeasts and fungi. Part type 4 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Publication

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Terminator guo

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM025_P2_pTUP11

Plasmid

#216450

Purpose

Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Publication

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

Promoter tup11

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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