We narrowed to 10,232 results for: gnas

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pLKO_AU1.HSV.Ollas_mCherry-NLS

Plasmid

#178223

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

AU1.HSV.Ollas and Nuclear Localization Signal

Promoter

eF1a

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_AU1.HA.V5_mCherry-NLS

Plasmid

#178221

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

AU1.HA.V5 and Nuclear Localization Signal

Promoter

eF1a

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_AU1.HA.Ollas_mCherry-NLS

Plasmid

#178218

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

AU1.HA.Ollas and Nuclear Localization Signal

Promoter

eF1a

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_AU1.C.Ollas_mCherry-NLS

Plasmid

#178212

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

AU1.C.Ollas and Nuclear Localization Signal

Promoter

eF1a

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_HSV.Ollas.S_mCherry-NLS

Plasmid

#178276

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

HSV.Ollas.S and Nuclear Localization Signal

Promoter

eF1a

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_HA.HSV.Ollas_mCherry-NLS

Plasmid

#178259

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

HA.HSV.Ollas and Nuclear Localization Signal

Promoter

eF1a

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO_HA.HSV.NWS_mCherry-NLS

Plasmid

#178258

Purpose

Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.

Depositor

Brian Brown

Article

Dhainaut et al Cell. 2022 Mar 10. pii: S0092-8674(22)00

Insert

nPC Tagged mCherry-NLS

Use

Lentiviral

Tags

HA.HSV.NWS and Nuclear Localization Signal

Promoter

eF1a

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pEN_hU6miR-G12-E

Plasmid

#25757

Purpose

Entry vector with human U6 promoter driving mouse G alpha 12 miR30-based shRNA.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

G alpha 12 miR-shRNA (Gna12 Mouse)

Use

Entry vector

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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pCEP4-CCR5(R5gp120-Hi)

Plasmid

#98954

Purpose

Mammalian expression plasmid for myc-tagged human CCR5 mutant R5gp120-Hi

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CCR5 (CCR5 Human)

Tags

Signal/leader sequence from Influenza A virus HA …

Expression

Mammalian

Mutation

R5gp120-Hi variant: P8L, I9E, I12M, S17E, P19D, Q…

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pTAMAHISTEV_PfeAR480A-Q482A

Plasmid

#128946

Purpose

expresses R480A Q482A mutant of PfeA in E. coli

Depositor

James Naismith

Article

Moynie et al Nat Commun. 2019 Aug 14;10(1):3673. doi:

Insert

ferric enterobactin receptor

Tags

TEV protease cleavable 7xHis and TamA

Expression

Bacterial

Mutation

Signal peptide sequence has been removed (1-75), …

Promoter

T7

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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hFlt1 Y1333F

Plasmid

#86037

Purpose

human VEGFR1 with Y to F mutation at position 1333

Depositor

Christie Thomas

Article

Raikwar et al PLoS One. 2014 Nov 11;9(11):e112794. doi

Insert

human Flt1 Y1333F (FLT1 Human)

Tags

FLAG, HA, and Myc

Expression

Mammalian

Mutation

Y1333F; HA tag is inserted downstream of signal …

Promoter

CMV

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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L4207 TNFR2 NTEVp chain in PolyTX-mNeonGreen

Plasmid

#244175

Purpose

Constitutive expression of a MESA NTEVp chain encoding (N to C): TNFR2SS-3xFLAG-TNFR2ECD-mCD28TMD-NTEVp(75S)

Depositor

Joshua Leonard

Article

Edelstein et al Nat Chem Biol. 2025 Aug 22:10.1038/s4158

Insert

MESA NTEVp chain with human TNFR2 SS and ECD, murine CD28 TMD, and NTEVp (75S) (TNFRSF1B Synthetic, Human)

Use

Synthetic Biology

Tags

TNFR2 signal peptide - 3xFLAG

Expression

Mammalian

Mutation

NTEVp_75S

Promoter

hEF1a

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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Mirror GFP[ENE(WT)-mascRNA](pAVA2965)

Plasmid

#239351

Purpose

Expresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of wild-type MALAT1 ENE-mascRNA reporter.

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

MALAT1 ENE(WT)-mascRNA

Expression

Mammalian

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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Mirror RFP[ENE(WT)-mascRNA](pAVA2987)

Plasmid

#239348

Purpose

Expresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of wild-type MALAT1 ENE-mascRNA reporter.

Depositor

Andrei Alexandrov

Article

Che et al Nat Commun. 2025 May 22;16(1):4741. doi:

Insert

MALAT1 ENE(WT)-mascRNA

Expression

Mammalian

Available Since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-sp-myc-hSema4D-Q497P

Plasmid

#190655

Purpose

Expresses Human Sema4D with N terminal myc after signal peptide containing 497P mutation

Depositor

Andrew Newman, Victor Tarabykin

Article

Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

Insert

Sema4D (SEMA4D Human)

Tags

myc

Expression

Mammalian

Mutation

497P

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-sp-flag-hSema4D-Q497P

Plasmid

#190657

Purpose

Expresses Human Sema4D with N terminal flag after signal peptide containing 497P mutation

Depositor

Andrew Newman, Victor Tarabykin

Article

Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

Insert

Sema4D (SEMA4D Human)

Tags

flag

Expression

Mammalian

Mutation

497P

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti-TO-HA-Δ37 PPM1H-mito

Plasmid

#208375

Purpose

Lentiviral expression of human Δ37-PPM1H with C-terminal mito signal

Depositor

Suzanne Pfeffer

Article

Yeshaw et al Proc Natl Acad Sci U S A. 2023 Oct 31;12

Insert

HA-delta37 PPM1H-mito (PPM1H Human)

Use

Lentiviral

Expression

Mammalian

Mutation

delta37

Available Since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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pYI2216

Plasmid

#228349

Purpose

2,4-Diacetylphloroglucinol (DAPG)-regulatable ALX-0081 expression

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

Insert

MFalpha(2xAdv)-LE-ALX-0081-3A-FLAG-His

Use

Affinity Reagent/ Antibody

Tags

AAA linker followed by FLAG-His6 and MF_ secretio…

Expression

Yeast

Promoter

2,4-Diacetylphloroglucinol-regulatable synthetic …

Available Since

March 28, 2025

Availability

Academic Institutions and Nonprofits only

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pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3

Plasmid

#221112

Purpose

FAP tagged STE3 (N-terminally tagged, optimized for yeast expression) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

MFA-IgKappa-FAPoptim-STE3

Expression

Yeast

Promoter

STE3

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pRS415-TEF1pr-MFA-Igkappa-FAPoptim-STE3

Plasmid

#221114

Purpose

FAP tagged STE3 (N-terminally tagged, optimized for yeast expression) under the Tef1 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

MFA-IgKappa-FAPoptim-STE3

Expression

Yeast

Promoter

TEF1

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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