user-defined upper limit for the number of target sequences returned
Alignment
region of similarity between target and query sequences
E-value
a BLAST statistic representing the significance of an alignment, values close to zero
indicate high sequence similarity with low probability of the similarity occurring by chance
Identities
the number of exact nucleotide matches over the alignment, expressed as a fraction
and a percentage
Query Coverage
the length of the query sequence that matches the target sequence in the
alignment
Bit Score
a BLAST statistic measuring the quality of an alignment, higher values indicate a
more significant match
Span
the length of the alignment, including gaps
About Search by Sequence
Search by Sequence performs a nucleotide-nucleotide BLAST search against Addgene’s plasmid sequence database.
BLAST returns plasmids with similarity to the query sequence.
Results are sorted by E-value, a statistic from BLAST that describes the significance of a match.
Lower values are considered better matches.
FASTA headers and numbers at the beginning of each line will be removed.
The query should only contain DNA characters.
The minimum query length is 30 nucleotides. Support for short sequence queries is under development.
Tips for Success
Inspect the percent identity, query coverage, and alignment details to determine if a result match is satisfactory.
Visit the corresponding plasmid webpage to view additional details about a matching plasmid.
If no results are returned, try a different isoform or region of the desired sequence. There may not be a match in our database.
You can adjust the Max Results setting on the results page from 25 to 500. If many sequences share the same top E-value,
only a truncated set of equally high-scoring matches will be shown. Set the Max Results to 500 to see more matches.
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All-in-one CRISPR/Cas system with 74 individual elements. 63 elements target cancer-related genes with FDA-approved drug treatments, and 3 elements target key medulloblastoma driver genes.
The human pgPoison library induces skipping of “poison” cassette exons and corresponding upstream constitutive exons by targeting 3' splice sites with paired gRNAs (pgRNAs).
A genome-wide CRISPR library covering 13,501 genes (98.8% of the Drosophila melanogaster genome), among which 8,989 genes are targeted by three or more independent single guide RNAs (sgRNAs).
This pooled lentiviral CRISPR knockout library targets E-boxes genome-wide and can be applied for identification of essential MYC binding sites in a wide range of human cells.
B. subtilis CRISPRi Essential Gene Knockdown Library: Arrayed library that uses CRISPRi to sterically hinder transcription and therefore knock down expression of essential genes.
B. subtilis Single Gene Deletion Library - Kanamycin: Arrayed deletion library in which each non-essential gene has been individually replaced with a kanamycin resistance cassette.
Integrative constructs for (over-) expression of (heterologous) genes in both laboratory and industrial yeast (S. cerevisiae) strains; Allow for selection in auxotrophic and prototrophic yeast strains
Used for the general dereplication of antibiotics in natural product extracts. Plasmids constitutively express individual resistance genes that target the most commonly found antibiotics.
Four plasmids containing the cloning cassette, eight plasmids containing LSDs engineered to heterodimerize and their binding partners, and five plasmids with different light-activated Opto-caspase 9.
Three plasmids with the empty cloning cassette in configurations ABC, BAC or ACB, and 21 plasmids containing one of nine light-sensing domains (LSDs) or LSD binding partners.
Expanded tool kit for the auxin-inducible degron (AID) system in yeast ( S. cerevisiae). Includes tags for easy detection by antibodies/microscopy and a set of selection markers.