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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,224 results for: PAM

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Showing: 941 - 960 of 2224 results

  12. PET28-FnCas12a-EP16

    Plasmid
    #183636
    Purpose
    PET28-FnCas12a-EP16 can efficiently recognize a broad range of PAM sequences including YN (Y = C or T), TAC and CAA.
    Depositor
    Yunzi Luo
    Article
    Wang et al Biotechnol Bioeng. 2019 Jun;116(6):1463-
    Insert
    FnCas12a-EP16
    Use
    CRISPR
    Tags
    6×His Tag
    Expression
    Bacterial
    Mutation
    N607R, K613V, N617R, K180S, K660R, D616N
    Available Since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    183636-plasmid-map-sequence-id-375575

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  15. pGEX-2T-FRB-W2027F

    Plasmid
    #73341
    Purpose
    Point mutation Trp 2027 → Phe in the FKBP12-rapamycin binding (FRB) domain of mTOR
    Depositor
    Jie Chen
    Article
    Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72
    Insert
    FKBP12-rapamycin binding (FRB) domain of MTOR (MTOR Human)
    Tags
    GST-tagged
    Expression
    Bacterial
    Mutation
    FRB domain only aa2015-2114; W2027F
    Available Since
    April 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  16. pGEX-2T FRB Y2105A

    Plasmid
    #68726
    Purpose
    Point mutation Tyr 2105 → Ala in the FKBP12-rapamycin binding (FRB) domain of mTOR
    Depositor
    Jie Chen
    Article
    Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72
    Insert
    FKBP12-rapamycin binding (FRB) domain of MTOR (MTOR Human)
    Tags
    GST-tagged
    Expression
    Bacterial
    Mutation
    FRB domain only aa2015-2114; Y2105A
    Available Since
    April 19, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 941 - 960 of 2224 results