Addgene

We narrowed to 18,762 results for: Vars

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pCSJ125

Plasmid

#104344

Purpose

Express yeast MDH2-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.

Depositor

Alexander Varshavsky

Article

Chen et al Science. 2017 Jan 27;355(6323). pii: 355

Insert

MDH2

Use

Tags

3xFLAG

Expression

Yeast

Mutation

Promoter

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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OeNL-H2B_pcDNA3

Plasmid

#89527

Purpose

Orange color variant of bright luminescent protein, fused to histone 2B

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

OeNL

Use

Tags

histone 2B

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pFA1

Plasmid

#46789

Purpose

To express Fun30 in trans in budding yeast

Depositor

Patrick Varga-Weisz

Article

Neves-Costa et al PLoS One. 2009 Dec 1;4(12):e8111. doi: 1

Insert

FUN30 (FUN30 Budding Yeast)

Use

Tags

V5

Expression

Yeast

Mutation

Promoter

PGAL1

Available since

Aug. 6, 2013

Availability

Academic Institutions and Nonprofits only
OeNL-Actin_pcDNA3

Plasmid

#89524

Purpose

Orange color variant of bright luminescent protein, fused to actin

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

OeNL

Use

Tags

Actin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pNB774

Plasmid

#60114

Purpose

Green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA)

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

GrLuc

Use

Unspecified

Tags

ClpXP degron and Nuclear localization signal (NLS…

Expression

Mutation

Promoter

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pNB763

Plasmid

#60158

Purpose

Firefly luciferase (Promega) driven by LYS9 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc

Use

Tags

Expression

Yeast

Mutation

FLuc has A4V & S504G (no functional effect)

Promoter

LYS9pr

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pNB761

Plasmid

#60156

Purpose

Firefly luciferase (Promega) driven by ADE17 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc

Use

Tags

Expression

Yeast

Mutation

FLuc has A4V & S504G (no functional effect)

Promoter

ADE17pr

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pNB775

Plasmid

#60115

Purpose

Yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA)

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

YeLuc

Use

Unspecified

Tags

ClpXP degron and Nuclear localization signal (NLS…

Expression

Mutation

Promoter

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pNB776

Plasmid

#60117

Purpose

Red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA)

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

RdLuc

Use

Unspecified

Tags

ClpXP degron and Nuclear localization signal (NLS…

Expression

Mutation

Promoter

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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MAC_N_TLX3

Plasmid

#167807

Purpose

Expresses TLX3 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

TLX3 (TLX3 Human)

Use

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_N_PAX9

Plasmid

#167789

Purpose

Expresses PAX9 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

PAX9 (PAX9 Human)

Use

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_N_TLX2

Plasmid

#167806

Purpose

Expresses TLX2 in mammalian cells

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

TLX2 (TLX2 Human)

Use

Tags

MAC

Expression

Mammalian

Mutation

no mutations

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pCSJ182

Plasmid

#159723

Purpose

Express Gid4 (YBR105C) in yeast two hybrid plasmid, pGADCg

Depositor

Alexander Varshavsky

Article

Chen et al Science. 2017 Jan 27;355(6323). pii: 355

Insert

GID4 (VID24 Budding Yeast)

Use

Yeast two-hybrid assay

Tags

3xflag and Gal4AD

Expression

Yeast

Mutation

remove stop codon

Promoter

ADH1

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCSJ392

Plasmid

#159722

Purpose

Express Gid10 (YGR066C) in yeast two hybrid plasmid, pGADCg

Depositor

Alexander Varshavsky

Article

Melnykov et al Proc Natl Acad Sci U S A. 2019 Aug 6;116

Insert

GID10 (GID10 Budding Yeast)

Use

Yeast two-hybrid assay

Tags

3xflag and Gal4AD

Expression

Yeast

Mutation

remove stop codon

Promoter

ADH1

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA1

Plasmid

#145369

Purpose

Gateway destination vector for cell-free and mamalian expression with N-term 8xHis and eGFP tag

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Type

Empty backbone

Use

Tags

His and eGFP

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shNFS1_4

Plasmid

#102966

Purpose

Suppression of NFS1

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shNFS1_4 (NFS1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pSpCas9 (BB)-2A-GFP gamma-tubulin sg

Plasmid

#104437

Purpose

Knockdown the expression of gamma-tubulin in human cells

Depositor

Maria Alvarado-Kristensson

Article

Lindstrom et al Biochim Biophys Acta. 2017 Oct 16. pii:

Insert

gamma-tubulin sgRNA

Use

Tags

The gamma-tubulin sgRNA is coexpressed with 3xFLA…

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO.1P shNFS1_3

Plasmid

#102965

Purpose

Suppression of NFS1

Depositor

Richard Possemato

Article

Alvarez et al Nature. 2017 Nov 22. doi: 10.1038/nature

Insert

shNFS1_3 (NFS1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
ReNL-Actin_pcDNA3

Plasmid

#89531

Purpose

Red color variant of bright luminescent protein, fused to actin

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

ReNL

Use

Tags

Actin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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tubulin-OeNL_pcDNA3

Plasmid

#89523

Purpose

Orange color variant of bright luminescent protein, fused to b-tubulin

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

OeNL

Use

Tags

b-tubulin

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge