We narrowed to 63,044 results for: cloning
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Plasmid#116423PurposeLentiviral expression of KRAS G12DDepositorAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pAAV-mDlx-GCaMP6f-Fishell-2
Plasmid#83899PurposeGCaMP6f expression in forebrain GABA-ergic interneurons under the control of the mDlx enhancer elementDepositorHas serviceAAV1, AAV9, and AAV RetrogradeInsertGCaMP6f
UseAAVExpressionMammalianPromotermDlxAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCS6-SMASh-YFP
Plasmid#68852PurposeExpresses SMASh tag fused to YFP at N terminus in mammalian cellsDepositorInsertSMASh-YFP
TagsSMASh tagExpressionMammalianPromoterCMVAvailable sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pT3-EF1a-Bmi1
Plasmid#31783DepositorPublicationAvailable sinceOct. 21, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a T7pCONS TIR-2 sfGFP
Plasmid#154464PurposepET28a with a design flaw in the T7 promoter fixed, and a synthetically evolved TIR. Contains the coding sequence for sfGFPDepositorInsertsuper folder GFP
TagsHis-thrombinExpressionBacterialPromoterT7Available sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1-GINKO1
Plasmid#113112PurposeMammalian expression of genetically encoded green fluorescent potassium ion indicator GINKO1DepositorInsertGINKO1
ExpressionMammalianPromoterCMVAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
NYESO beta/alpha into TRAC HDRT Source (pTR 169)
Plasmid#112021PurposeDNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-alphaDepositorInsertNYESO beta/alpha into TRAC HDRT
UseCRISPR and Synthetic BiologyAvailable sinceSept. 1, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR_PGK_LaG16_2_synNotch_Gal4VP64
Plasmid#85421PurposeLentiviral vector for expression of LaG16_2 GFP nanobody Gal4VP64 synthetic NotchDepositorInsertLaG16_2 GFP nanobody synNotch Gal4VP64
UseLentiviralPromoterPGKAvailable sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJC175e
Plasmid#79219PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPPSP gIII, luxAB
ExpressionBacterialPromoterPPSPAvailable sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Hsp68-LacZ-Gateway
Plasmid#37843DepositorTypeEmpty backboneUseMouse TargetingPromoterMouse Hsp68 minimal promoterAvailable sinceSept. 20, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pFRT/TO/HIS/FLAG/HA-ALKBH5
Plasmid#38073DepositorPublicationInsertALKBH5 alkB, alkylation repair homolog 5 (E. coli) (ALKBH5 Human)
TagsHIS/FLAG/HAExpressionMammalianMutationcDNA clone MGC:71143 IMAGE:6339184 with longer 3&…Available sinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEMTEZ-TeTxLC
Plasmid#32640DepositorPublicationInsertTetanus toxin light chain
ExpressionBacterialAvailable sinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDNA3.1-hMcl-1
Plasmid#25375DepositorAvailable sinceAug. 23, 2010AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Flag-HA-USP22
Plasmid#22575DepositorPublicationAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-MMLVgag-3xNES-ABE8e
Plasmid#181751PurposeMMLV-Gag fused to 3xNES-ABE8e with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.DepositorInsertMMLVgag-3xNES-ABE8e
TagsFlagExpressionMammalianPromoterCMVAvailable sinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CoV2-M-IRES-E
Plasmid#177938PurposeTransient mammalian expression of codon optimized SARS-CoV-2 Membrane and Envelope proteins (original Wuhan Hu1 sequence). Used for generating RNA packaging virus-like particles.DepositorExpressionMammalianAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-ABE7.9
Plasmid#102918Purposeexpresses ABE7.9 in mammalian cellsDepositorInsertABE7.9
TagsNLSExpressionMammalianMutationsee manuscriptPromoterCMVAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
lentiGuide-Hygro-mTagBFP2
Plasmid#99374PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A mTagBFP2 from EF-1a promoter. 3rd generation lentiviral backbone.DepositorInsertsS. pyogenes sgRNA cassette
Hygro-P2A-mTagBFP2
UseCRISPR and LentiviralExpressionMammalianPromoterEF-1a and hU6Available sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1_sfCherry2(1-10)
Plasmid#82602PurposeExpresses sfCherry2(1-10) fusion in mammalian cellsDepositorInsertsfCherry2(1-10)
ExpressionMammalianMutationchanged Glu 118 to Gln, changed Thr 128 to IlePromoterCMVAvailable sinceJan. 17, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMLRT
Plasmid#78234PurposeExpresses Murine Leukemia Reverse Transcriptase (MLRT)DepositorInsertMurine leukemia reverse transcriptase
ExpressionBacterialPromoterJ23102Available sinceAug. 6, 2016AvailabilityAcademic Institutions and Nonprofits only