We narrowed to 15,130 results for: pET

1. pET16b-Antp

   Plasmid

   #86866

   Purpose

   Bacterial expression of fusion protein with cell penetrating peptide, antennapedia fragment, at C-terminus.

   Depositor

   Michael Chin

   Article

   Chien et al Genesis. 2014 Jul;52(7):695-701. doi: 10

   Insert

   Linker-Antp
   

   Tags

   Antp

   Expression

   Bacterial

   Available Since

   April 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET15-EF1p_mFAP2b

   Plasmid

   #159469

   Purpose

   Encodes for EF1p_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.

   Depositor

   David Baker

   Insert

   EF1p_mFAP2b
   

   Tags

   6xHis-thrombin-TEV

   Expression

   Bacterial

   Available Since

   Dec. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETM30-ARC105

   Plasmid

   #133426

   Purpose

   Human ARC105 coding sequence in vector for in vitro transcription and protein expression, with T7 promoter.

   Depositor

   Gerhard Wagner

   Article

   Yang et al Nature. 2006 Aug 10;442(7103):700-4. doi

   Insert

   PCQAP (MED15 Human)
   

   Expression

   Bacterial

   Mutation

   Contains amino acids 5-78 fused to C-terminal of …

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETM30-C9ORF78_F8A

   Plasmid

   #193369

   Purpose

   Expression of C9ORF78 F8A variant in E.coli with N-terminal His6-GST-tag which is cleavable with TEV protease

   Depositor

   Markus Wahl

   Article

   Bergfort et al Nat Commun. 2022 Mar 3;13(1):1132. doi:

   Insert

   C9ORF78_F8A (C9orf78 Human)
   

   Tags

   His6-GST-TEV

   Expression

   Bacterial

   Mutation

   F8A, the mutation is located in the binding inter…

   Promoter

   T7

   Available Since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-cMSPdH45

   Plasmid

   #199767

   Purpose

   Expresses split-intein fused MSPdH45 for circularization during expression (internal His-Tag)

   Depositor

   Franz Hagn

   Article

   Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

   Insert

   cMSPdH45
   

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   internal His-Tag

   Available Since

   July 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET22b-T5-FB-E25TAG

   Plasmid

   #176545

   Purpose

   Expresses IgG affinity protein FB with a TAG stop codon at E25 position

   Depositor

   Han Xiao

   Article

   Yu et al Bioconjug Chem. 2018 Nov 21;29(11):3522-

   Insert

   IgG affinity protein
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   change glutamic acid 25 to amber stop codon

   Promoter

   T5

   Available Since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETM6-P.accABCD

   Plasmid

   #66866

   Purpose

   Encodes accA, accB, accC, accD in Pseudo-operon configuration.

   Depositor

   Mattheos Koffas

   Article

   Xu et al Nat Commun. 2013;4:1409. doi: 10.1038/nc

   Inserts

   accA
   accB
   accC
   accD
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET15b-hnRNP-F

   Plasmid

   #23021

   Depositor

   Douglas Black

   Article

   Chou et al Mol Cell Biol. 1999 Jan . 19(1):69-77.

   Insert

   hnRNP-F (HNRNPF Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   June 4, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pET28 sf:mid.11

   Plasmid

   #191921

   Purpose

   Encodes a GFP design, sf:mid.11

   Depositor

   Sarel Fleishman

   Article

   Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

   Insert

   sf:mid.11
   

   Tags

   His Tag

   Expression

   Bacterial

   Mutation

   Q69M Y145F T167I L220V V224I

   Promoter

   T7

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28 sf:mid.7

    Plasmid

    #191933

    Purpose

    Encodes a GFP design, sf:mid.7

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    sf:mid.7
    

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    S72T Y145F V224I

    Promoter

    T7

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28 acid.6

    Plasmid

    #191912

    Purpose

    Encodes a GFP design, acid.6

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    acid.6
    

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Q69L S72A Y145M T167V H181L

    Promoter

    T7

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28 sf:acid.3

    Plasmid

    #191926

    Purpose

    Encodes a GFP design, sf:acid.3

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    sf:acid.3
    

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    T65S Q69L S72A T108V Y145M V224I

    Promoter

    T7

    Available Since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28 mid.7

    Plasmid

    #191900

    Purpose

    Encodes a GFP design, mid.7

    Depositor

    Sarel Fleishman

    Article

    Weinstein et al Nat Commun. 2023 May 20;14(1):2890. doi:

    Insert

    mid.7
    

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    S72T Y145F V224I

    Promoter

    T7

    Available Since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET19b_4CL + STS

    Plasmid

    #139790

    Purpose

    expression of biosynthetic enzymes 4CL and STS

    Depositor

    Roman Jerala

    Article

    Fink et al AMB Express. 2020 May 24;10(1):97. doi:

    Inserts

    4CL
    STS
    

    Expression

    Bacterial

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28_6xHis-PARP1delZn1

    Plasmid

    #173933

    Purpose

    Bacterial expression of PARP1 lacking Zn1 domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delZn1 (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 1-96; V762A

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28_6xHis-PARP1-HD

    Plasmid

    #174809

    Purpose

    Bacterial expression of isolated PARP1 HD subdomain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-HD (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    V762A

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. 3322_pETcon_SARS2_Omicron-BA2

    Plasmid

    #184409

    Purpose

    yeast surface display of the SARS-CoV-2 Omicron BA.2 variant RBD

    Depositor

    Jesse Bloom

    Article

    Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

    Insert

    SARS-CoV-2 Omicron BA.2 Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)
    

    Tags

    HA and c-Myc

    Expression

    Yeast

    Mutation

    G339D+S371F+S373P+S375F+T376A+D405N+R408S+K417N+N…

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28a-ChmN

    Plasmid

    #221348

    Purpose

    Express ChmN in E. coli

    Depositor

    Emily Balskus

    Article

    Crooke et al J Am Chem Soc. 2024 Jun 12;146(23):16268

    Insert

    chmN
    

    Tags

    His6

    Expression

    Bacterial

    Available Since

    July 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pETSUMO2_Rhodoplanes-bGSDM

    Plasmid

    #212129

    Purpose

    For expression of the bacterial gasdermin (bGSDM) encoded by a Rhodoplanes species. Protein is expressed with an N-terminal SUMO2 tag.

    Depositor

    Philip Kranzusch

    Article

    Johnson et al Science. 2022 Jan 14;375(6577):221-225.

    Insert

    Rhodoplanes bGSDM
    

    Tags

    6xHis-SUMO2

    Expression

    Bacterial

    Mutation

    N-terminal methionine deleted and replaced by sin…

    Promoter

    T7

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pETSUMO2_Bacteroidetes-bGSDM

    Plasmid

    #212124

    Purpose

    For expression of the bacterial gasdermin (bGSDM) encoded by a Bacteroidetes (metagenomic) species. Protein is expressed with an N-terminal SUMO2 tag.

    Depositor

    Philip Kranzusch

    Article

    Johnson et al Science. 2022 Jan 14;375(6577):221-225.

    Insert

    Bacteroidetes bGSDM
    

    Tags

    6xHis-SUMO2

    Expression

    Bacterial

    Mutation

    N-terminal methionine deleted and replaced by sin…

    Promoter

    T7

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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