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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 23,646 results for: nes

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Showing: 9601 - 9620 of 23646 results

  2. pPyCAG-EGFP-TM-IRES-Pac

    Plasmid
    #183603
    Purpose
    Mammalian expression vector for membrane-tethered EGFP from the CAG promoter. Confers puromycin resistance.
    Depositor
    Sally Lowell
    Publication
    Malaguti et al Development. 2022 May 26. pii: 275525. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP-TM
    Tags
    Human PDGFRB transmembrane domain and Mouse IgGK …
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    183603-plasmid-map-sequence-id-361589

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  3. pPyPGK-EGFP-TM-IRES-Pac

    Plasmid
    #183604
    Purpose
    Mammalian expression vector for membrane-tethered EGFP from the mouse Pgk1 promoter. Confers puromycin resistance.
    Depositor
    Sally Lowell
    Publication
    Malaguti et al Development. 2022 May 26. pii: 275525. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP-TM
    Tags
    Human PDGFRB transmembrane domain and Mouse IgGK …
    Expression
    Mammalian
    Promoter
    Mouse Pgk1
    Available since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    183604-plasmid-map-sequence-id-361590

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  4. pPyPGK-Myc-LaG17-SynNotch-tTA-IRES-Ble

    Plasmid
    #183607
    Purpose
    Mammalian expression vector: mouse Pgk1 promoter driving expression of a SynNotch receptor comprising anti-GFP nanobody (LaG17), mouse Notch1 core, tTA. Confers bleomycin/zeocin resistance.
    Depositor
    Sally Lowell
    Publication
    Malaguti et al Development. 2022 May 26. pii: 275525. d
    Insert
    LaG17 GFP nanobody SynNotch tTA
    Tags
    Myc and human CD8a signal sequence
    Expression
    Mammalian
    Promoter
    Mouse Pgk1
    Available since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    183607-plasmid-map-sequence-id-361593

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  5. Ai163 (TIT2L-GC6s-ICL-TPT) targeting vector

    Plasmid
    #114430
    Purpose
    Target a Cre-dependent GCaMP6s cassette and a tdTomato-P2A-tTA2 cassette to the mouse TIGRE locus
    Depositor
    Hongkui Zeng
    Publication
    Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GCaMP6s, tdTomato, tTA2
    Use
    Cre/Lox and Mouse Targeting
    Promoter
    TRE2, CAG
    Available since
    Jan. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    114430-plasmid-map-sequence-id-227317

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  6. pdCas9-M-3A2

    Plasmid
    #73435
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3A2.
    Depositor
    Mattheos Koffas
    Publication
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor 3A2 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available since
    March 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    73435-plasmid-map-sequence-id-135340

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  8. pcDNA-dCas9-p300 Core (1645/1646 RR/EE)

    Plasmid
    #61359
    Purpose
    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; mutation 1645/1646 RR/EE) driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Synthetic, S. pyogenes, Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9; 1645/1646 RR/EE m…
    Promoter
    CMV
    Available since
    April 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    61359-plasmid-map-sequence-id-99792

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  10. pEGFP-MyosinVb-Ctail

    Plasmid
    #110170
    Purpose
    Dominant negative effect by competing with MyosinVb C-terminal tail interactions
    Depositor
    Jose Esteban
    Publication
    Correia et al Nat Neurosci. 2008 Apr;11(4):457-66. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Myosin Vb (Myo5b Rat)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    amino acids 1262-1670
    Promoter
    CMV
    Available since
    May 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    110170-plasmid-map-sequence-id-210548

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  15. AD-003

    Plasmid
    #25502
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    AD-003 (NTMT1 Human)
    Tags
    6x His and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 9601 - 9620 of 23646 results