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DCT.VV.Orf74

Plasmid

#12762

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf74

Expression

Mammalian

Available Since

Dec. 6, 2006

Availability

Academic Institutions and Nonprofits only
pAG221 MDFIC 3'UTR mut

Plasmid

#12051

Depositor

David Bartel

Article

Farh et al Science. 2005 Dec 16. 310(5755):1817-21.

Insert

N22 3'UTR mut (MDFIC Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutations in microRNA recognition sites (seed mat…

Available Since

June 8, 2006

Availability

Academic Institutions and Nonprofits only
pSB819-PKR-hum-FYS

Plasmid

#20046

Depositor

Harmit Malik

Article

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

Protein kinase R (EIF2AK2 Human)

Expression

Yeast

Mutation

L394F, F489Y

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pSB819-PKR-hum-FYA

Plasmid

#20049

Depositor

Harmit Malik

Article

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

Protein kinase R (EIF2AK2 Human)

Expression

Yeast

Mutation

L394F, F489Y, S492A

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pSB819-PKR-hum-LYA

Plasmid

#20050

Depositor

Harmit Malik

Article

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

Protein kinase R (EIF2AK2 Human)

Expression

Yeast

Mutation

F489Y, S492A

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pCAG-GCaMP6f

Plasmid

#249680

Purpose

Expresses GCaMP6f under the CAG promoter

Depositor

Takeshi Imai

Article

Inagaki et al Nat Methods. 2026 Mar 12. doi: 10.1038/s

Insert

GCaMP6f

Expression

Mammalian

Promoter

CAG

Available Since

March 13, 2026

Availability

Academic Institutions and Nonprofits only

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pAT1089-(PB-4_EF1α-Blast_2A_rtTA3 [HA-NLS-dCas9-NLS])

Plasmid

#251108

Purpose

Expression of dCas9 component of Casilio-i system

Depositor

Chia-Lin Wei

Article

Taghbalout et al Cancer Cell. 2025 Dec 8;43(12):2191-2205

Insert

dCas9

Tags

HA

Expression

Mammalian

Mutation

N/A

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pSFV_eGFP-P2A-luc2pre (3'UTR)

Plasmid

#240254

Purpose

Non-attenuated SFV vector with eGFP-LUC transgene. Package VLVs with an envelope-expressing vector such as pCMV-VSV-G, or a transgene-responsive VSV-G expression vector.

Depositor

Gregory Neely

Article

Cole et al Nat Commun. 2025 May 7;16(1):4250. doi:

Insert

EGFP-P2A-firefly luciferase

Expression

Mammalian

Promoter

SFV subgenomic promoter

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pSFV_rTetR-VP16 (3'UTR)_RLEloop

Plasmid

#240257

Purpose

Attenuated PROTEUS vector with rtTA-3G transgene. Package VLVs with an envelope-expressing vector such as pCMV-VSV-G, or a transgene-responsive VSV-G expression vector.

Depositor

Gregory Neely

Article

Cole et al Nat Commun. 2025 May 7;16(1):4250. doi:

Insert

rTetR-VP16

Expression

Mammalian

Mutation

VP16[aa437-447]

Promoter

SFV subgenomic promoter

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pSFV_TetR-VP16 (3'UTR)_RLEloop

Plasmid

#240259

Purpose

Attenuated PROTEUS vector with tTA transgene. Package VLVs with an envelope-expressing vector such as pCMV-VSV-G, or a transgene-responsive VSV-G expression vector.

Depositor

Gregory Neely

Article

Cole et al Nat Commun. 2025 May 7;16(1):4250. doi:

Insert

TetR-VP16

Expression

Mammalian

Mutation

VP16[aa437-447]

Promoter

SFV subgenomic promoter

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Split SNORA70

Plasmid

#200644

Purpose

Expressed a eGFP sequence where a chimeric intron with the sequence reference of SNORA70 (NR_000011) was inserted

Depositor

Claire Francastel

Article

Bogard et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

Insert

Split GFP with chimeric intron containing SNORA70

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Split SNORA40

Plasmid

#200645

Purpose

Expressed a eGFP sequence where a chimeric intron with the sequence reference of SNORA40 (NR_002973.1) was inserted

Depositor

Claire Francastel

Article

Bogard et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

Insert

Split GFP with chimeric intron containing SNORA40

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pME234

Plasmid

#238104

Purpose

Expresses ME-ABE ABE7.10-HK with SpCas9 (NG

Depositor

Alexis Komor

Article

Evanoff et al Nat Biotechnol. 2026 Mar 18. doi: 10.103

Insert

TadA*7.10(Y123H/N157K)-m_SpnCas9(D10A)

Use

CRISPR

Tags

SV40 bpNLS and SV40 bpNLS-P2A-mCherry

Expression

Mammalian

Mutation

TadA modifications include Y123H, N157K; NG-nCas9…

Available Since

Jan. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pMXs-SNAP-p53DD

Plasmid

#244168

Purpose

Retroviral expression of SNAP-p53DD

Depositor

Kate Galloway

Article

Lende-Dorn et al Cell Rep. 2025 Sep 23;44(9):116226. doi:

Insert

SNAP-p53DD (Trp53 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pGuide-RUNX3

Plasmid

#246659

Purpose

Guide RNA for human RUNX3

Depositor

Joriene de Nooij

Article

Malka-Gibor et al Stem Cell Reports. 2026 Jan 2:102753. do

Insert

RUNX3 guide RNA (RUNX3 Human)

Use

CRISPR

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pPD686 A2UCOE-EnAsCas12a-BFP

Plasmid

#249159

Purpose

Overexpression of EnAsCas12a-BFP with blasticidin resistance gene. Contains minimal A2UCOE and Cp36 recombination site.

Depositor

Michael Bassik

Article

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

A2UCOE-EnAsCas12a-TagBFP

Use

CRISPR; Cp36 donor dna

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pEF1-FRT-HaloTag-RPA194-E593Q

Plasmid

#247349

Purpose

Expresses HaloTag-RPA194-E593Q

Depositor

Kazuhiro Maeshima

Article

Ide et al Sci Adv. 2020 Oct 14;6(42). pii: 6/42/ea

Insert

RPA194 (POLR1A) (POLR1A Human)

Tags

HaloTag

Expression

Mammalian

Mutation

RPA194 (POLR1A), mutated with E593Q

Promoter

EF1α

Available Since

Jan. 6, 2026

Availability

Academic Institutions and Nonprofits only

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p-att-ef1a-MS2-RecT-dCas9-BSD

Plasmid

#226108

Purpose

Using ef-1a promoter, expresses dCas9 and RecT protein that intended to be tethered via MS2 gRNA hairpin to dCas9, and Blasticidin selection

Depositor

George Church

Article

Oliynyk et al Commun Biol. 2025 Dec 16;8(1):1778. doi:

Insert

BSD

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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CFP-LacI-NUT-KS

Plasmid

#238279

Purpose

For overexpression of CFP-LacI-NUT-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

CFP-LacI-NUT-KS

Expression

Mammalian

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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CFP-LacI-NUT-KS-FtoG

Plasmid

#238280

Purpose

For overexpression of CFP-LacI-NUT-KS-FtoG

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

CFP-LacI-NUT-KS-FtoG

Expression

Mammalian

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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