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pLAS3W-S100P

Plasmid

#125009

Purpose

express in mammalian cell

Depositor

Ruey-Hwa Chen

Article

Tan et al Oncogene. 2019 Jul;38(28):5612-5626. doi

Insert

S100P (S100P Human)

Expression

Mammalian

Promoter

CAG

Available Since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-CaaX(Hras)

Plasmid

#108886

Purpose

Expression of membrane localized mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

mCherry-CaaX(Hras)

Use

Can be used for mammalian expression or in vitro …

Expression

Mammalian

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pJC175e

Plasmid

#79219

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PPSP gIII, luxAB

Expression

Bacterial

Promoter

PPSP

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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BPK2660

Plasmid

#70709

Purpose

Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pCAG_smFP HA

Plasmid

#59759

Purpose

encodes multiple copies of an epitope tag HA

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

smFP _HA

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only

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MIGR1-U6gRNA1-Filler-v2

Plasmid

#237400

Purpose

For inserting a single guide RNA or later cloning two U6-gRNA cassettes with MIGR1-U6gRNA1-Filler-v1.

Depositor

Zuoming Sun

Article

Zhong et al STAR Protoc. 2025 Jun 20;6(2):103831. do

Type

Empty backbone

Use

CRISPR and Retroviral

Expression

Mammalian

Promoter

U6, PGK

Available Since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti6.2-ccdB-3xFLAG-V5

Plasmid

#87071

Purpose

Lentiviral Gateway-compatible expression vector backbone with a C-terminal 3xFLAG-V5 tag

Depositor

Susan Lindquist, Mikko Taipale

Article

Taipale et al Cell. 2014 Jul 17;158(2):434-48. doi: 10

Type

Empty backbone

Use

Lentiviral

Tags

3xFLAG-V5

Expression

Mammalian

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pBIG1a

Plasmid

#80611

Purpose

pBIG1a - Step1 vector a (biGBac system for expression of protein complexes in insect cells)

Depositor

Jan-Michael Peters

Article

Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

Type

Empty backbone

Expression

Insect

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pLOX-CW-CRE

Plasmid

#12238

Depositor

Didier Trono

Article

Cudre-Mauroux et al Hum Gene Ther. 2003 Nov 1. 14(16):1525-3

Insert

CRE

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

CMV promoter is upstream of nls-CRE. LoxP in 3&#x…

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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SBE-NLS-mCherry PGK-rtTA3

Plasmid

#195332

Purpose

Lentiviral construct of TGFβ reporter (nuclear mCherry) and hPGK driven rtTA3

Depositor

Elaine Fuchs

Article

Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

Inserts

NLS-mCherry
rtTA3

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hPGK and miniCMV

Available Since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pmiRFP713-tubulin

Plasmid

#136561

Purpose

Human alpha-tubulin labeling with a near-infrared fluorescent protein miRFP713

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

miRFP713-tubulin

Tags

miRFP713

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG_smFP Myc

Plasmid

#59757

Purpose

encodes multiple copies of an epitope tag Myc

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

smFP _Myc

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pMJ915

Plasmid

#69090

Purpose

Express Streptococcus pyogenes Cas9 carrying two C-terminal SV40 NLS

Depositor

Jennifer Doudna

Article

Lin et al Elife. 2014 Dec 15;3:e04766. doi: 10.755

Insert

cas9

Tags

MBP, hexa His, and two SV40 NLS

Expression

Bacterial

Promoter

T7

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCherry-mNrp1

Plasmid

#21934

Depositor

Guido Serini

Article

Valdembri et al PLoS Biol. 2009 Jan 27. 7(1):e25.

Insert

neuropilin 1 (Nrp1 Mouse)

Tags

Cherry and His

Expression

Mammalian

Available Since

Oct. 13, 2009

Availability

Academic Institutions and Nonprofits only
pcs2-GFP-IRES-mCherry

Plasmid

#226098

Purpose

Protein stability reporter construct for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.

Depositor

Michael Rape

Article

Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-UFM1

Plasmid

#134639

Purpose

expression of HA-tagged human UFM1 wildtype

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

UFM1 (UFM1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pBEN276

Plasmid

#69150

Purpose

Integration of luciferase operon in Tn7 insertion site of Gram negative bacteria

Depositor

Pierre Germon

Article

Howe et al BMC Microbiol. 2010 Jul 23;10:197. doi:

Insert

luxCDABE

Use

Luciferase

Promoter

frr

Available Since

Dec. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pGY18

Plasmid

#221708

Purpose

Empty Cas9 vector for multiplex gene editing in fungi

Depositor

Kyle Pomraning

Article

Yuan et al Front Bioeng Biotechnol. 2024 Oct 16;12:

Type

Empty backbone

Use

CRISPR; Aspergillus

Expression

Bacterial

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pME-nlsGFP-P2A

Plasmid

#80808

Purpose

nuclear localization signal fused to N-terminus of GFP (nlsGFP) without stop codon, followed by P2A

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

minimal cytomegalovirus immediate early enhancer/promoter

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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AChE-pcDNA

Plasmid

#106442

Purpose

Mammalian expression of murine acetylcholinesterase

Depositor

Georg Weber

Article

Syed et al Clin Exp Metastasis. 2008;25(7):787-98.

Insert

acetylcholinesterase (ACHE Human)

Expression

Mammalian

Mutation

WT

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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