Addgene

We narrowed to 1,425 results for: CAG promoter

Showing: 961 - 980 of 1425 results

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pCA-T-intron(Neo)-G(LNL)

Plasmid

#36908

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

T (i.e., ATG, start codon as a first exon)
GFP
beta-globin intron
Neo

Use

Tags

Expression

Mammalian

Mutation

deleted nucleotides before nucleotide 275 and ins…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pBT267_(pCA-ATG-intron-tTA2-TRE-tdT3Myc)

Plasmid

#36879

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

tTA2
tdT-3Myc

Use

Tags

3 Myc tags

Expression

Mammalian

Mutation

insertion of a beta-globin intron with a loxP sit…

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

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pCA-T-intron(Neo)-G(FLLFLNL)

Plasmid

#40021

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

T (ATG)
GFP
beta-globin intron
Neo

Use

Tags

Expression

Mutation

deleted nucleotides 1-274 and insertion of FRT, …

Promoter

CAG (chicken beta globin promoter and CMV enhance…

Available since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only

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JPF0446

Plasmid

#124022

Purpose

BxBI Destination vector with H2B::GFP expressed from CAG promoter

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

BXBI_pCAG-H2B::msfGFP-bghpA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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U6-sgR26-eCas9-T2A-BlastR

Plasmid

#172493

Purpose

For CRISPR-assisted HDR, Rosa26 locus

Depositor

Tyler Jacks

Article

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Insert

eCas9, BlastR and sgRNA targeting the Rosa26 (R26) safe harbor locus

Use

CRISPR, Lentiviral, and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_sgFLNB-SK2

Plasmid

#119873

Purpose

gRNA expression vector

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

sgFLNB-SK2 (FLNB Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A-del1352-1369-siRNAres_K

Plasmid

#146743

Purpose

Mammalian Expression of HsTNRC6A-del1352-1369-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

HsTNRC6A-del1352-1369-siRNAres (TNRC6A Human)

Use

Tags

Expression

Mammalian

Mutation

one non silent mutation K1578E compared to the se…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6A_1242-1709-F1359A_K

Plasmid

#146742

Purpose

Mammalian Expression of HsTNRC6A_1242-1709-F1359A

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

HsTNRC6A_1242-1709-F1359A (TNRC6A Human)

Use

Tags

Expression

Mammalian

Mutation

five non silent mutations D1289G, G1515A, S1516G,…

Promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-Neo-GFP/Foxa1

Plasmid

#105506

Purpose

MSCV-driven retroviral Foxa1 expression

Depositor

Christopher Vakoc

Article

Roe et al Cell. 2017 Aug 24;170(5):875-888.e20. do

Insert

Foxa1 (Foxa1 Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-Neo-GFP/Flag-Gata5

Plasmid

#105508

Purpose

MSCV-driven retroviral Gata5 expression (Flag-tagged)

Depositor

Christopher Vakoc

Article

Roe et al Cell. 2017 Aug 24;170(5):875-888.e20. do

Insert

Gata5 (Gata5 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mutation

Promoter

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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MSCV-Neo-GFP/V5-Foxa1

Plasmid

#105507

Purpose

MSCV-driven retroviral Foxa1 expression (V5-tagged)

Depositor

Christopher Vakoc

Article

Roe et al Cell. 2017 Aug 24;170(5):875-888.e20. do

Insert

Foxa1 (Foxa1 Mouse)

Use

Retroviral

Tags

V5

Expression

Mutation

Promoter

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only
ISDra2-TnpB-mCherry-U6-ωRNA*

Plasmid

#212967

Purpose

Expression vector for expression of ISDra2-TnpB driven by chicken β-actin promoter and mCherry driven by CMV promoter

Depositor

Chunlong Xu

Article

Li et al Nat Commun. 2024 Jan 27;15(1):831. doi:

Inserts

ISDra2-TnpB-T2A-mCherry
ωRNA*

Use

CRISPR

Tags

NLS and NLS-FLAG

Expression

Mammalian

Mutation

Promoter

CAG, T7 and U6

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shMki67

Plasmid

#160958

Purpose

Mki67 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Mki67 (Mki67 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pSLQ1371-sgYAP-2

Plasmid

#121423

Purpose

sgYAP-2 sequence: GAGATGACTTCCTGAACAGTG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

Depositor

Ovijit Chaudhuri, Stanley Qi

Article

Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

Insert

sgYAP-2

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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ATP1A1_G3_EBFP_Nick_Dual_sgRNA

Plasmid

#178094

Purpose

Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and EBFP Nick sgRNAs from two independent U6 promoters.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G3 + EBFP nick sgRNAs

Use

CRISPR; Prime editing

Tags

Expression

Mammalian

Mutation

Promoter

Tandem U6 promoters

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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ATP1A1_G3_EGFP_Nick_Dual_sgRNA

Plasmid

#178095

Purpose

Control vector for coselection for PE3b in human cells. Tandem expression of ATP1A1 G3 and EGFP Nick sgRNAs from two independent U6 promoters.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G3 + EGFP nick sgRNAs

Use

CRISPR; Prime editing

Tags

Expression

Mammalian

Mutation

Promoter

Tandem U6 promoters

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pSC22

Plasmid

#104796

Purpose

CRISPR/Cas9 2xplex gRNA targeting Medtr2g101120, Medtr2g101130 (Acre1). Also expresses Cas9 from Gmubi promoter from AtUBQ10 promoter

Depositor

Nevin Young

Article

Curtin et al Plant Physiol. 2017 Feb;173(2):921-931.

Insert

Medtr2g101120, Medtr2g101130

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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FgH1tUTG_muBim_Ex3

Plasmid

#85533

Purpose

Inducible expression of guide RNA (muBim_Ex3) with fluorescent GFP reporter

Depositor

Marco Herold

Article

Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

Insert

mu Bim

Use

Tags

Expression

Mutation

Promoter

H1t

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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OA-1050G (white)

Plasmid

#132420

Purpose

express arrays of gRNA targeting White under dU6-3 promoter

Depositor

Omar Akbari

Article

Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

Insert

white gRNA array

Use

Tags

Expression

Insect

Mutation

Promoter

U6-3

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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ATP1A1_G2_Dual_sgRNA

Plasmid

#173201

Purpose

Coselection for ABE or NHEJ in human cells. Vector for tandem expression of ATP1A1 G2 sgRNA with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G2 sgRNA + user-specified sgRNA

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Tandem U6 promoters

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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