We narrowed to 7,678 results for: CCH

1. pROS12

   Plasmid

   #107926

   Purpose

   hph based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

   Depositor

   Jean-Marc Daran

   Article

   Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

   Insert

   gRNA-CAN1.Y, gRNA-ADE2.Y
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pROS15

   Plasmid

   #107929

   Purpose

   nat based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

   Depositor

   Jean-Marc Daran

   Article

   Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

   Insert

   gRNA-CAN1.Y, gRNA-ADE2.Y
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pROS14

   Plasmid

   #107928

   Purpose

   LEU2 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

   Depositor

   Jean-Marc Daran

   Article

   Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

   Insert

   gRNA-CAN1.Y, gRNA-ADE2.Y
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pROS13

   Plasmid

   #107927

   Purpose

   kan based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

   Depositor

   Jean-Marc Daran

   Article

   Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

   Insert

   gRNA-CAN1.Y, gRNA-ADE2.Y
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUDP044

   Plasmid

   #101168

   Purpose

   pUDP004 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes SeATF1 and SeATF2 and Spcas9D147Y P411T in S. pastorianus (HH-gRNASeATF1-HDV-linker-HH-gRNASeATF2-HDV)

   Depositor

   Jean-Marc Daran

   Article

   de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

   Insert

   polycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting SeATF1 and 2 in S. pastorianus
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   ScTDH3

   Available Since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. p426_Cas9_gRNA-R-ARS607c

   Plasmid

   #87409

   Purpose

   p426_Cas9_gRNA-ARS607c without ribozyme All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS607c sequence CTATTTTTGCTTTCTGCACA in yeast chromosome 6.

   Depositor

   Aindrila Mukhopadhyay

   Article

   Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

   Insert

   Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS607c
   

   Use

   CRISPR

   Promoter

   ADH1, pTyrosine

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIFEX-A2aGH

   Plasmid

   #160542

   Purpose

   pITY4 derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

   Depositor

   Michelle O'Malley

   Article

   Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

   Insert

   Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Human, Budding Yeast)
   

   Expression

   Yeast

   Mutation

   N/A

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEFEX1-A2aGH

   Plasmid

   #160543

   Purpose

   pYES2 derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

   Depositor

   Michelle O'Malley

   Article

   Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

   Insert

   Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Human, Budding Yeast)
   

   Expression

   Yeast

   Mutation

   N/A

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEFEX2-A2aGH

   Plasmid

   #160544

   Purpose

   pYES2 derivative reporter plasmid encoding PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

   Depositor

   Michelle O'Malley

   Article

   Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

   Insert

   PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Human, Budding Yeast)
   

   Expression

   Yeast

   Mutation

   N/A

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEFEX3-A2aGH

    Plasmid

    #160545

    Purpose

    pYES2 derivative reporter plasmid encoding PREV1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

    Depositor

    Michelle O'Malley

    Article

    Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

    Insert

    PREV1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Human, Budding Yeast)
    

    Expression

    Yeast

    Mutation

    N/A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCFEX1-A2aGH

    Plasmid

    #160546

    Purpose

    pYC2/CT derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

    Depositor

    Michelle O'Malley

    Article

    Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

    Insert

    Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Human, Budding Yeast)
    

    Expression

    Yeast

    Mutation

    N/A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCFEX2-A2aGH

    Plasmid

    #160547

    Purpose

    pYC2/CT derivative reporter plasmid encoding PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

    Depositor

    Michelle O'Malley

    Article

    Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

    Insert

    PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Human, Budding Yeast)
    

    Expression

    Yeast

    Mutation

    N/A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCFEX3-A2aGH

    Plasmid

    #160548

    Purpose

    pYC2/CT derivative reporter plasmid encoding PREV1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

    Depositor

    Michelle O'Malley

    Article

    Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

    Insert

    PREV1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Human, Budding Yeast)
    

    Expression

    Yeast

    Mutation

    N/A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKP134 [pRS406-YBR139Wp-YBR139W(S219,D415,H474A)-GFP-ADH1t]

    Plasmid

    #106469

    Purpose

    Expresses Atg42/Ybr139w(S219,D415,H474A) with a C-terminal GFP tag in yeast cells

    Depositor

    Daniel Klionsky

    Article

    Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

    Insert

    YBR139W(S219,D415,H474A)
    

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Mutation

    Changed Serine 219, Aspartate 415 and Histidine 4…

    Promoter

    YBR139W

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. p426_Cas9_gRNA-R-ARS805a

    Plasmid

    #87408

    Purpose

    p426_Cas9_gRNA-ARS805a without ribozyme All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS805a sequence TTATTTGAATGATATTTAGT in yeast chromosome 8.

    Depositor

    Aindrila Mukhopadhyay

    Article

    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

    Insert

    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS805a
    

    Use

    CRISPR

    Promoter

    ADH1, pTyrosine

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TFORF0145

    Plasmid

    #143015

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PHOX2B (PHOX2B Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1_ spike_del19

    Plasmid

    #155297

    Purpose

    Expression of spike with C-term deletion of 19 aa, used to generate high efficiency SARS-CoV-2-pseudotyped lentiviral particles

    Depositor

    Raffaele De Francesco

    Article

    Conforti et al Mol Ther. 2021 Sep 20. pii: S1525-0016(2

    Insert

    SARS-Cov2 spike_deleted (S SARS-Cov2)
    

    Use

    Generation of sars-cov-2-pseudotyped lentiviral p…

    Mutation

    Deletion in C-term 19 aa of spike

    Promoter

    CMV

    Available Since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. PB-CA retrofitted

    Plasmid

    #159093

    Purpose

    piggyBac ITR's from PB-CA retrofitted with a linker

    Depositor

    Mario Capecchi

    Type

    Empty backbone

    Use

    Piggybac

    Expression

    Mammalian

    Available Since

    Nov. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBluescript-PB

    Plasmid

    #133885

    Purpose

    piggyBac ITR's in high copy number pBluescript backbone plasmid

    Depositor

    Mario Capecchi

    Type

    Empty backbone

    Use

    Piggybac

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PB-CA sfGFP

    Plasmid

    #159094

    Purpose

    PB-CA sfGFP piggyBac transposon

    Depositor

    Mario Capecchi

    Insert

    sfGFP
    

    Use

    Piggybac

    Expression

    Mammalian

    Available Since

    Nov. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge