We narrowed to 165,564 results for: addgene

1. pCMV_SNAP_24

   Plasmid

   #238191

   Purpose

   Empty lentiviral vector for expressing protein sequences fused to SNAP-tag and FTH1 (24-mer), under a CMV promoter

   Depositor

   Feng Zhang

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   SNAP-FTH1 and SV40

   Expression

   Mammalian

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. MigR1 GFP Mouse PHGDH

   Plasmid

   #220591

   Purpose

   Retroviral mammalian expression vector with GFP for overexpression of mouse PHGDH

   Depositor

   Russell Jones

   Insert

   Ms PHGDH (Phgdh Mouse)
   

   Use

   Retroviral

   Available Since

   Feb. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCG-ACTB-C4

   Plasmid

   #229846

   Purpose

   Expresses wild-type Cas9 and gRNA for ACTB gene.

   Depositor

   Natsuko Chiba

   Insert

   guide RNA for ACTB gene
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCG-NT2

   Plasmid

   #229848

   Purpose

   Expresses wild-type Cas9 and gRNA with non-target sequence.

   Depositor

   Natsuko Chiba

   Insert

   guide RNA with non-target sequence
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 9, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. 8VL7

   Plasmid

   #220883

   Purpose

   Baculovirus expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   TREX1 (TREX1 Human)
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 20, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. cTRE-LSL-GFP-IRES-Cas9-sgPtenX1.1

   Plasmid

   #135669

   Purpose

   Introduce Dox/Cre-inducible (TRE promoter followed by lox-stop-lox) Cas9 cDNA plus Pten-targeting sgRNA into (ES) cells by recombination-mediated cassette exchange

   Depositor

   Florian Karreth

   Insert

   sgPten
   

   Use

   Mouse Targeting

   Available Since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pE4F1.1.0-gDNA

   Plasmid

   #132451

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   E4F1 (E4F1 Human)
   

   Use

   CRISPR

   Available Since

   Dec. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSBbi-Pur-LPAR

   Plasmid

   #125205

   Purpose

   SB-transposon plasmid for stable expression of lipid raft-targeted FRET-based kinase activity reporter with EV spacer, with AarI cassette for cloning of a kinase-target peptide

   Depositor

   Eric Davis

   Insert

   Lyn-Phospho-Activity-Reporter, Empty
   

   Use

   Transposon

   Expression

   Mammalian

   Promoter

   EF1a/RPBSA

   Available Since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. 5K29 (PP-BRD3)

   Plasmid

   #101883

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   PP-BRD3
   

   Tags

   6xHis, TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 30, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. 5TCM (PP-BRD3)

    Plasmid

    #101888

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PP-BRD3
    

    Tags

    6xHis, TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 30, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. 4QNS

    Plasmid

    #61957

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PF08_0034:MAC04J-B11:C229648
    

    Tags

    MHHHHHHSSGRENLYFQG

    Expression

    Bacterial

    Mutation

    See comments

    Promoter

    T7-lacO

    Available Since

    March 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

12. PP-AHA1

    Plasmid

    #28123

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PFC0360w:MAC03H-A08:C212775 (PFC0360w Plasmodium falciparum)
    

    Tags

    His tag with TEV site

    Expression

    Bacterial

    Available Since

    Feb. 28, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pX552-GFP

    Plasmid

    #107023

    Purpose

    pX552-CMV-GFP. AAV plasmid for sgRNA cloning, with GFP fusion facilitating FACS sorting of cells. Replaced the hSyn promoter in pX552 from Zhang lab with a CMV promoter.

    Depositor

    Alex Hewitt

    Insert

    GFP
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKM343

    Plasmid

    #69762

    Purpose

    Retroviral expression of CENP-I

    Depositor

    Iain Cheeseman

    Insert

    CENP-I (CENPI Human)
    

    Use

    Retroviral

    Tags

    eGFP-TEV-S

    Expression

    Mammalian

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

15. 9AVA

    Plasmid

    #220884

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    TREX1 (TREX1 Human)
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 20, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pUC18

    Plasmid

    #50004

    Purpose

    pUC cloning vector

    Depositor

    Joachim Messing

    Article

    Norrander et al Gene. 1983 Dec;26(1):101-6.

    Type

    Empty backbone

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pINDUCER20-hMYOD1-3xHA (#730)

    Plasmid

    #78328

    Purpose

    Doxycycline-inducible lentiviral overexpression of human MYOD1 ORF with a 3xHA tag

    Depositor

    Louis Kunkel, Matthew Alexander

    Insert

    MYOD1 (MYOD1 Human)
    

    Use

    Lentiviral

    Tags

    3x HA tag

    Promoter

    TRE2

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)

    Plasmid

    #140082

    Purpose

    Entry cloning vector for in vitro transcription or expression of SpCas9 sgRNAs from a T7 promoter

    Depositor

    Keith Joung

    Insert

    pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
    

    Use

    In vitro transcription (mrna synthesis)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR_5xUAS-BFP-WPRE_PGK-HygR-bGHpolyA

    Plasmid

    #216665

    Purpose

    Expresses Gal4 inducible BFP, with hygromycin resistance

    Depositor

    Leonardo Morsut

    Inserts

    BFP
    HygromycinR
    

    Use

    Lentiviral

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pINDUCER20-FLAG-hMYOD1 (#800)

    Plasmid

    #78330

    Purpose

    Doxycycline-inducible lentiviral overexpression of human MYOD1 ORF with a FLAG tag

    Depositor

    Louis Kunkel, Matthew Alexander

    Insert

    MYOD1 (MYOD1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Promoter

    TRE2

    Available Since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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