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pCMV-dCas12a-ABE7

Plasmid

#193644

Purpose

Expression of base editor dCas12a-ABE7 in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-TadA-TadA*7.10, EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-SARS-CoV-2 S1 RBD

Plasmid

#167013

Purpose

Gateway-compatible Entry vector encoding spike (S1) RBD domain from SARS-CoV-2

Depositor

Spyros Petrakis

Article

Gkekas et al J Chem Inf Model. 2024 Nov 13. doi: 10.1

Insert

S (S SARS-CoV-2 isolate Wuhan-Hu-1)

Expression

Mammalian

Mutation

No stop codon

Available Since

April 5, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-hSyn-DIO-GRAB_CRF0.5

Plasmid

#208660

Purpose

Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5 in a cre-dependent manner

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5

Use

AAV

Promoter

hSyn

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_CRF0.5

Plasmid

#208657

Purpose

Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5 in neurons

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF0.5

Use

AAV

Promoter

hSyn

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pINSPECT:NEAT1-total_SigP:NLuc

Plasmid

#197267

Purpose

Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human NEAT1 locus (both isoforms). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.

Depositor

Gil Westmeyer

Article

Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

Insert

NEAT1-total homology arms

Use

CRISPR, Luciferase, Synthetic Biology, and Unspec…

Expression

Mammalian

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCR3-FLAG-TRAF6-C70A

Plasmid

#43926

Depositor

Bryant Darnay

Article

Lamothe et al J Biol Chem. 2007 Feb 9;282(6):4102-12.

Insert

TNF Receptor-Associated Factor 6 (Traf6 Mouse)

Tags

2x FLAG

Expression

Mammalian

Mutation

C70A

Available Since

Jan. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pWZL Sox2 R74P

Plasmid

#26355

Depositor

Matthew Meyerson

Article

Bass et al Nat Genet. 2009 Nov . 41(11):1238-42.

Insert

Sox2 (SOX2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

R74P

Available Since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only
pSB305-K3L-vac-HA

Plasmid

#20054

Depositor

Harmit Malik

Article

Elde et al Nature. 2009 Jan 22;457(7228):485-9. doi

Insert

K3L

Tags

HA

Expression

Yeast

Available Since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only
pGM48DEST

Plasmid

#74757

Purpose

Encodes for the QS repressor gene, splice linker, and mCherry reporter gene

Depositor

Jordan Ward, Keith Yamamoto

Article

Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

Inserts

QS
Gateway cassette

Expression

Worm

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pEB2-mCherry-L

Plasmid

#104002

Purpose

Plasmid encoding mCherry-L

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mCherry-L

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to DsRed (MSKGEE DNLA IIKEF… V…

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO/FLAG-HA-SBP-TNRC6A-NES-mut

Plasmid

#42046

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6A (TNRC6A Human)

Tags

FLAG, HA, and SBP

Expression

Mammalian

Mutation

F951A, F955A, I958A, F960A

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pSUPER-CXCR4

Plasmid

#42052

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

CXCR4 shRNA (CXCR4 )

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Feb. 25, 2013

Availability

Academic Institutions and Nonprofits only

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HA-RNF126

Plasmid

#138647

Purpose

Mammalian expression of 2HA-tagged RNF126

Depositor

Jane McGlade

Article

Smith et al Exp Cell Res. 2014 Jan 15;320(2):219-32.

Insert

RNF126 (RNF126 Mouse)

Tags

HA-HA

Expression

Mammalian

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pBS_12x-601_MA2

Plasmid

#114359

Purpose

12x Widom 601 nucleosome sequence

Depositor

Scot Wolfe

Article

Swygert et al Proc Natl Acad Sci U S A. 2018 Dec 4;115

Insert

12X601-MA2

Use

Unspecified

Expression

Mammalian

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pRST11B-AS3_4

Plasmid

#102789

Purpose

Expresses tryptophanyl tRNA synthetase (ScWRS) and supressor tryptophanyl tRNA

Depositor

Andrew Ellington

Article

Abil et al Nat Protoc. 2017 Dec;12(12):2493-2512. d

Inserts

tryptophanyl tRNA synthetase (WRS1 Budding Yeast)
suppressor tRNA

Expression

Bacterial

Available Since

July 3, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-EPN-01*

Plasmid

#80039

Purpose

Expression of EPN-01*

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01*

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pSA101

Plasmid

#59787

Purpose

drives ZIF-1 protein in C. elegans neurons

Depositor

Jeremy Nance

Article

Armenti et al Development. 2014 Dec;141(23):4640-7. do

Inserts

rab-3 promoter
zif-1 coding (zif-1 Nematode)
unc-54 3' UTR

Available Since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-CMVenh synapsin-intron-eGFP

Plasmid

#247941

Purpose

AAV transfer plasmid encoding enhanced Green Fluoresence Protein control of the CMVie enhanced synapsin1 promoter

Depositor

Veerle Baekelandt

Article

Oliveras-Salva et al Mol Neurodegener. 2013 Nov 25;8:44. doi:

Insert

eGFP

Use

AAV

Promoter

CMVenh synapsin

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pSA073 HBB_IVS2-Cas9-BFP

Plasmid

#249134

Purpose

Overexpression of SpCas9-BFP with HBB IVS2 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site.

Depositor

Michael Bassik

Article

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

HBB_IVS2-Cas9-TagBFP (HBB S. pyogenes Cas9, synthetic, Human)

Use

CRISPR; Cp36 donor dna

Expression

Mammalian

Mutation

HBB IVS2 intron

Promoter

EF1a

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pSA097 HNRNPK_IVS3-Cas9-BFP

Plasmid

#249147

Purpose

Overexpression of SpCas9-BFP with HNRNPK IVS3 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site.

Depositor

Michael Bassik

Article

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

HNRNPK_IVS3-Cas9-TagBFP (HNRNPK S. pyogenes Cas9, synthetic, Human)

Use

CRISPR; Cp36 donor dna

Expression

Mammalian

Mutation

HNRNPK IVS3 intron

Promoter

EF1a

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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