We narrowed to 12,121 results for: VARS
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Plasmid#216285PurposeExpresses DoubleCatcher H-lock in bacterial cells. DoubleCatcher H-lock is a variant of DoubleCatcher in which each SpyCatcher003 moiety is separated by an alpha helical linker.DepositorInsertDoubleCatcher H-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
eNme2C-CBE6c
Plasmid#215831PurposeExpress CBE6c (with eNme2-C variant) in mammalian cellsDepositorInsertCBE6c-eNme2C-2xUGI
ExpressionMammalianAvailable SinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHis17_ParM _1
Plasmid#78201PurposeExpression of single-cysteine variant of plasmid segregation protein, ParM, as scaffold for ADP biosensorDepositorInsertparM (K33A I27C T174A T175N C287A)
TagsHis6ExpressionBacterialMutationK33A I27C T174A T175N C287APromoterunknownAvailable SinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL0_20 [panARS-opt ORI]
Plasmid#198933PurposeLevel 0 partDepositorInsertpanARS-opt ORI
UseSynthetic BiologyAvailable SinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
Ii-Str_puromycin
Plasmid#65309PurposeLuminal ER hook only (RUSH system)DepositorInsertInvariant chain (p33)
ExpressionMammalianMutationStreptavidin in its lumenal partPromoterCMVAvailable SinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+18)
Plasmid#199162Purposeused to express a supercharged GFP variant in E. coliDepositorInsertGFP(+18)
TagsMGHHHHHHGGAMutationE16R, D29K, E42K, E44K, D86K, D112K, T115K, D127R…PromoterT7Available SinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-6xHis-GFP(+12)
Plasmid#199161Purposeused to express a supercharged GFP variant in E. coliDepositorInsertGFP(+12)
TagsMGHHHHHHGGAMutationE16R, E44K, D86K, D112K, T115K, D127R, I138R, E15…PromoterT7Available SinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL3-LRF
Plasmid#66936PurposeDestination vector; gateway cassette cloned upstream of basal promoter driving luciferase. Forward orientation (attR1, attR2).DepositorTypeEmpty backboneUseLuciferaseExpressionMammalianPromoterSV-40Available SinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
CMV-CitroffRH
Plasmid#158546PurposeMammalian expression of nonbinding variant of inverse-response citrate sensorDepositorInsertCitroffRH
ExpressionMammalianPromotercmvAvailable SinceOct. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJH462
Plasmid#172154PurposepylBCD variant in pBAD backboneDepositorInsertPara (Sumo-tagged pylBCD); AraC
ExpressionBacterialMutationSUMO tag fused to N-terminus of PylBAvailable SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL2_166
Plasmid#199098PurposeLevel 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporterDepositorInsertRK2 ORI plus NGS barcode 12, gentamicin resistance cassette, mScarlet-I cassette, oriT
UseSynthetic BiologyAvailable SinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL2_167
Plasmid#199099PurposeLevel 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporterDepositorInsertpBBR1 ORI plus NGS barcode 13, gentamicin resistance cassette, mScarlet-I cassette, oriT
UseSynthetic BiologyAvailable SinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJH643
Plasmid#172168PurposeReporter plasmid for pylBCD variant in pBAD backboneDepositorInsertPara sfGFP.3TAG; Plpp pylS; PproK pylT; AraC; Cam
ExpressionBacterialMutationsfGFP(N39*, N135*, Y151*)Available SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDB007ns2a
Plasmid#99214PurposeAccessory plasmid for PACE of PylRS variants; negative selectionDepositorInsertPpsp [SD8] gIII; PproK tyrT(Opt,CUA); Ptet [SD4] T7RNAP(S12*,S203*)
Mutationamber codons at positions 12 and 203 of T7 RNA po…PromoterPpsp, PproK, and PtetAvailable SinceOct. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
LENG
Plasmid#111162PurposemiR-E (miR-30 variant)-based RNAi with GFP reporterDepositorTypeEmpty backboneUseRetroviralTagsGFPAvailable SinceMay 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-⍺-Lock
Plasmid#216286PurposeExpresses DoubleCatcher-⍺-Lock in bacterial cells. DoubleCatcher-⍺-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher ⍺-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-β-Lock
Plasmid#216287PurposeExpresses DoubleCatcher-β-Lock in bacterial cells. DoubleCatcher-β-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher β-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-γ-Lock
Plasmid#216288PurposeExpresses DoubleCatcher-γ-Lock in bacterial cells. DoubleCatcher-γ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher γ-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-δ-Lock
Plasmid#216289PurposeExpresses DoubleCatcher-δ-Lock in bacterial cells. DoubleCatcher-δ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher δ-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-DoubleCatcher-ε-Lock
Plasmid#216290PurposeExpresses DoubleCatcher-ε-Lock in bacterial cells. DoubleCatcher-ε-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.DepositorInsertDoubleCatcher ε-Lock
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only