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pKH163_pSmart-mNEON-YIPF4

Plasmid

#214982

Purpose

endogenous mneon tag for YIPF4

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

mNEON with YIPF4 homology (YIPF4 Human)

Use

CRISPR

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3PXD2A-5/5

Plasmid

#91464

Purpose

Protein expression and purification of human SH3 domain construct SH3PXD2A-5/5

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3PXD2A-5/5 (SH3PXD2A Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3PXD2A-1/5

Plasmid

#91351

Purpose

Protein expression and purification of human SH3 domain construct SH3PXD2A-1/5

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3PXD2A-1/5 (SH3PXD2A Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_SH3PXD2A-3/5

Plasmid

#91352

Purpose

Protein expression and purification of human SH3 domain construct SH3PXD2A-3/5

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SH3PXD2A-3/5 (SH3PXD2A Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pCB128

Plasmid

#205137

Purpose

Expression of mCherry-2xFYVE in Dictyostelium

Depositor

Jason King

Article

Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

Insert

2xFYVE (from human Hrs) (HGS Synthetic)

Use

Dictyostelium expression

Tags

mCherry

Available Since

Oct. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-mCherry-N

Plasmid

#210271

Purpose

N-terminal mCherry-tagging of inserts for inducible expression. Use with Flp-In T-Rex system.

Depositor

Niels Mailand

Article

Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

Type

Empty backbone

Tags

mCherry

Expression

Mammalian

Available Since

Dec. 6, 2023

Availability

Academic Institutions and Nonprofits only

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UKNP1.3.2

Plasmid

#97362

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available Since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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UKNP1.3.1

Plasmid

#97361

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available Since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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UKNP1.2.3

Plasmid

#97358

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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UKNP1.2.4

Plasmid

#97359

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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UKNP1.2.5

Plasmid

#97360

Purpose

Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

Depositor

Jonathan Ball

Article

Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

Insert

HCV E1E2

Expression

Mammalian

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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DsRed2_pLENTI-CAG-IRES-GFP

Plasmid

#177010

Purpose

Mammalian lentiviral expression vector encoding DsRed2

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

DsRed2

Use

Lentiviral

Promoter

CAG

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV 3xFLAG-LC3B2-GFP

Plasmid

#123109

Purpose

Expresses 3xFLAG-LC3B2-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3B2

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3 beta 2 (MAP1LC3B2 Human)

Tags

3xFLAG and EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pPMVAugw

Plasmid

#98300

Purpose

Enhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of CUP1 promoter.

Depositor

Claudia Vickers

Article

Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

Insert

TEFM1EfmvaE>TEBS1

Expression

Yeast

Available Since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pQE-nTR

Plasmid

#21812

Depositor

Wafik El-Deiry

Article

Kim et al J Biol Chem. 2004 Sep 17. 279(38):40044-

Insert

pQE-nTR (TNFSF10 Human)

Expression

Bacterial

Mutation

His tag and aa95-113 were removed from pQE-hTR

Available Since

Sept. 14, 2012

Availability

Academic Institutions and Nonprofits only
pHH0103_SNX9-1/1

Plasmid

#91513

Purpose

Protein expression and purification of human SH3 domain construct SNX9-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SNX9-1/1 (SNX9 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/V5 mmu-mir-137

Plasmid

#26327

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-137 (Mir137 Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-JNKAR1-NES

Plasmid

#84632

Purpose

Unimolecular, FRET-based JNK activity reporter; cytosol targeted

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

ECFP-FHA1-JNKsubDD-Citrine-NES

Tags

6xHis, Citrine, ECFP, Nuclear export signal (NES)…

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB2803

Plasmid

#63646

Purpose

pCfB2803 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

Depositor

Irina Borodina, Jerome Maury

Article

Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

Type

Empty backbone

Use

Cre/Lox

Expression

Yeast

Promoter

none

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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p-715MRPprom-MRPcucuc

Plasmid

#87199

Purpose

Expression of ectopic mutated human MRP RNA contstruct from the human RMRP promoter, also contains CMV-puro-t2A-mCherry expression cassette for selection/transfection efficiency

Depositor

Thomas Cech

Article

Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

Insert

RMRP (RMRP Human)

Expression

Mammalian

Mutation

GAGAG91-95 to CUCUC

Promoter

human RMRP

Available Since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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