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pAAV.hSynapsin.SF-iGluSnFR.A184S

Plasmid

#106174

Purpose

Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Has Service

AAV1 and AAV9

Insert

SF-iGluSnFR.A184S

Use

AAV

Mutation

GltI: A184S

Promoter

hSynapsin

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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YAP1-V5 in pLX304

Plasmid

#42555

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO (pEHA1339)

Plasmid

#209088

Purpose

Backbone plasmid for Tet Responsive transgene expression, FRT integration

Depositor

Vikram Khurana

Article

Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HNF1B-WT-Flag-UTR

Plasmid

#183239

Purpose

Expression of FLAG tagged HNF1B

Depositor

Jorge Ferrer

Article

Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

Insert

HNF1 homeobox B (HNF1B Human)

Tags

FLAG tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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CRISPR-SB

Plasmid

#177936

Purpose

Expresses SpCas9 and sgRNA. Can be integrated into the genome upon co-delivery of Sleeping Beauty transposase.

Depositor

Roland Rad

Article

Kaltenbacher et al Nat Protoc. 2022 Apr;17(4):1142-1188. do

Type

Empty backbone

Use

CRISPR; Transposon

Expression

Mammalian

Promoter

U6

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-ABE8.20m-nSpG-P2A-EGFP (KAC1160)

Plasmid

#185916

Purpose

pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

Depositor

Benjamin Kleinstiver

Article

Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

Insert

human codon optimized ABE8.20m-nSpG-P2A-EGFP

Use

In vitro transcription; t7 promoter

Tags

BPNLS and BPNLS-3xFLAG-P2A-EGFP

Expression

Mammalian

Mutation

nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…

Promoter

CMV and T7

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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evoFERNY-Cas9NG

Plasmid

#203329

Purpose

Plasmid for bacterial purification of codon optimized evoFERNY-Cas9NG

Depositor

Vijay Sankaran

Article

Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

Insert

evoFERNY-Cas9NG

Use

CRISPR

Tags

His-tag

Expression

Bacterial

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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shYAP1 # 1

Plasmid

#42540

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

YAP1 (YAP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only

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PGL4-4X Mut Renilla

Plasmid

#70040

Purpose

Control plasmid for testing 5'UTR folding elements

Depositor

Hans-Guido Wendel

Article

Wolfe et al Nature. 2014 Sep 4;513(7516):65-70. doi:

Insert

Random sequence matched for GC-content

Use

Luciferase

Tags

renilla luciferase

Expression

Mammalian

Promoter

SV40

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only
Luciferase

Plasmid

#213979

Purpose

Expresses Doxy Inducible Luciferase

Depositor

Coleman Lindsley

Article

Reilly et al Blood. 2021 Sep 9;138(10):898-911. doi:

Insert

Luciferase

Use

Lentiviral

Tags

6xHis affinity tag

Expression

Bacterial and Mammalian

Promoter

Tet-responsive promoter PTight, consisting of sev…

Available Since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-EGFP-NLS-FLAG-cGAS

Plasmid

#127659

Purpose

Over-express EGFP-NLS-FLAG-cGAS

Depositor

Nicolas Manel

Article

Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

Insert

cGAS (CGAS Human)

Use

Lentiviral

Tags

EGFP-NLS-Flag

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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pBT2-4xUAS:NICD-P2A-mRFP

Plasmid

#127594

Purpose

Zebrafish NICD was driven by 4xnrUAS, to be co-expressed with mRFP through P2A self-cleaving peptides.

Depositor

Longhou Fang

Article

Gu et al Science. 2019 Mar 8;363(6431):1085-1088.

Insert

NICD (notch1a Zebrafish)

Use

Zebrafish expression

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-HAPLN1

Plasmid

#200777

Purpose

Expresses full-length mouse HAPLN1 fused with cysteine-free GFP (cfGFP) under synapsin promoter

Depositor

Alexander Dityatev

Article

Ramsaran et al Science. 2023 May 5;380(6644):543-551. d

Has Service

AAV8

Insert

Hyaluronan And Proteoglycan Link Protein 1 (Hapln1 Mouse)

Use

AAV

Tags

cfGFP

Promoter

Synapsin

Available Since

June 14, 2023

Availability

Academic Institutions and Nonprofits only

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pLX317-SNAI1

Plasmid

#115446

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

SNAI1 (SNAI1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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ERBB2 gRNA (BRDN0001148320)

Plasmid

#76378

Purpose

3rd generation lentiviral gRNA plasmid targeting human ERBB2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ERBB2 (ERBB2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ERBB2 gRNA (BRDN0001148673)

Plasmid

#76377

Purpose

3rd generation lentiviral gRNA plasmid targeting human ERBB2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ERBB2 (ERBB2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ERBB3 gRNA (BRDN0001147088)

Plasmid

#77481

Purpose

3rd generation lentiviral gRNA plasmid targeting human ERBB3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ERBB3 (ERBB3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ERBB3 gRNA (BRDN0001147186)

Plasmid

#77479

Purpose

3rd generation lentiviral gRNA plasmid targeting human ERBB3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ERBB3 (ERBB3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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ERBB3 gRNA (BRDN0001149441)

Plasmid

#77480

Purpose

3rd generation lentiviral gRNA plasmid targeting human ERBB3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

ERBB3 (ERBB3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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shYAP1 # 2

Plasmid

#42541

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

YAP1 (YAP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Feb. 8, 2013

Availability

Academic Institutions and Nonprofits only

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