We narrowed to 90,284 results for: Mal;

1. pAAV-hSyn-mRuby3-6xFLAG

   Plasmid

   #161613

   Purpose

   Cell morphology marker construct. Contains a red fluorescent protein mRuby3 and six FLAG tags.

   Depositor

   Edward Boyden

   Article

   Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

   Insert

   mRuby3-6xFLAG
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Human synapsin 1 promoter

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiCRISPRv1_SDHA

   Plasmid

   #177980

   Purpose

   lentiviral vector expressing Cas9 and a sgRNA targeting SDHA

   Depositor

   David Sabatini, Jessica Spinelli

   Article

   Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

   Insert

   sgRNA targeting SDHA (SDHA Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   U6

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. px330-CD4

   Plasmid

   #136938

   Purpose

   NHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human CD4; induce CD4+ deletion rearrangement by pairing w/ px330-GAPDH

   Depositor

   Jeremy Stark

   Article

   Bhargava et al J Biol Chem. 2019 Nov 21. pii: RA119.010

   Insert

   sgRNA targeting CD4 (CD4 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pL-CRISPR.SFFV.PAC

   Plasmid

   #57829

   Purpose

   Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, SFFV Promoter driven

   Depositor

   Benjamin Ebert

   Article

   Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

   Inserts

   SpCas9
   Sp sgRNA scaffold
   SFFV
   P2A-PAC
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Available Since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-hA3A-BE3-Y130F

   Plasmid

   #113428

   Purpose

   Expresses hA3A-BE3-Y130F in mammalian cells

   Depositor

   Jia Chen

   Article

   Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

   Insert

   hA3A-BE3-Y130F (APOBEC3A Human, S. pyogenes and Bacteriophage PBS2)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   hAPOBEC3A_Y130F

   Promoter

   CMV

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. nLYN267-gs10-NLS-VPR

   Plasmid

   #188265

   Purpose

   Plasmid ensures constitutive expression of the N-terminal fragment of split LYN kinase domain (aa 268-512), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

   Depositor

   Roman Jerala

   Article

   Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

   Insert

   nLYN267 (LYN Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. aCat DeltaTS (delta570 to 670 aa in aCat TS WT)

   Plasmid

   #101300

   Purpose

   FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin. See Depositor Comments for plasmid construction.

   Depositor

   Alpha Yap

   Article

   Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

   Insert

   alpha-Catenin
   

   Expression

   Mammalian

   Mutation

   Contains amino acids 1-509, 670-698, and 699-906 …

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAY19 (OsTIR1(F74A) mAID-EGFP-NLS)

   Plasmid

   #140535

   Purpose

   OsTIR1(F74A)-P2A-mAID-EGFP-NLS TOL2

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   mAID-EGFP-NLS TOL2
   

   Expression

   Mammalian

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV_UdgX-Anc689-UdgX-nCas9-RBMX

   Plasmid

   #163552

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-Anc689-UdgX-nCas9-RBMX
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)

   Available Since

   Dec. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. T7-VEE-CFP

    Plasmid

    #193357

    Purpose

    Self-replicating RNA vector expressing CFP fluorescent protein + PuroR

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    CFP
    

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hNR2F2-RFP

    Plasmid

    #22926

    Depositor

    Edward Callaway

    Article

    Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

    Insert

    human nuclear receptor subfamily 2 group F member 2 promoter driving DsRedExpress (NR2F2 Human)
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    May 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-Tag2-Sox9 T236A

    Plasmid

    #111455

    Purpose

    Transient expression of Flag-tagged human Sox9 with T236A mutation

    Depositor

    Sharon Pine

    Article

    Hong et al Nucleic Acids Res. 2016 Oct 14;44(18):88

    Insert

    Sox9 (SOX9 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T236A mutation

    Promoter

    CMV

    Available Since

    June 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEB3-miRFP670nano

    Plasmid

    #127433

    Purpose

    EB3 (microtubule-associated protein) labeling with near-infrared fluorescent protein miRFP670nano

    Depositor

    Vladislav Verkhusha

    Article

    Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

    Insert

    EB3-miRFP670nano (MAPRE3 Human, Synthetic)
    

    Tags

    miRFP670nano

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.1 MP1 shRNA

    Plasmid

    #26632

    Depositor

    David Sabatini

    Article

    Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

    Insert

    MP1 shRNA (LAMTOR3 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    Nov. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

15. Frt-V5-hspB7

    Plasmid

    #63108

    Purpose

    Expression of HSPB7 (small HSP) tagged with V5 in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb7 (HSPB7 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. PB-CAG-DDdCas9VPP300-T2A-GFP-IRES-Neo

    Plasmid

    #102887

    Purpose

    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-P300-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB-CAG-DDdCas9VPPH-T2A-GFP-IRES-Neo

    Plasmid

    #102888

    Purpose

    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAT15547_PEmax-SuperFi-Cas9

    Plasmid

    #184377

    Purpose

    Mammalian expression of SuperFi-Cas9 PEmax prime editor

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

    Insert

    PEmax-SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    SV40 NLS and c-myc NLS

    Expression

    Mammalian

    Mutation

    H840A, R221K, N394K, Y1010D, Y1013D, Y1016D, V101…

    Promoter

    CMV

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMSCV neo WT Akt1

    Plasmid

    #14549

    Depositor

    Joan Brugge

    Article

    Irie et al J Cell Biol. 2005 Dec 19. 171(6):1023-34

    Insert

    Akt1 (Akt1 Mouse)
    

    Use

    Retroviral

    Tags

    HA tag

    Expression

    Mammalian

    Available Since

    April 12, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pCMV_RBMX-eA3A-UdgX-nCas9

    Plasmid

    #163546

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    RBMX-eA3A-UdgX-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)

    Available Since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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