We narrowed to 118,874 results for: mpi

1. pPB_EF1a_mNeon

   Plasmid

   #212653

   Purpose

   piggyBac vector to express mNeon

   Depositor

   Paul Blainey

   Article

   Najia et al bioRxiv 2024.10.01.616098

   Insert

   mNeon
   

   Use

   Piggybac

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFLAG-VSVG

   Plasmid

   #212641

   Purpose

   Expression vector for FLAG-VSVG

   Depositor

   Paul Blainey

   Article

   Najia et al bioRxiv 2024.10.01.616098

   Insert

   VSVG
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. P30.pA.NGFR-mSrtA.mCMV.hPGK.GFP.wpre

   Plasmid

   #226728

   Purpose

   backbone vector, GFP expression

   Depositor

   Ferdinando Pucci

   Article

   Hamilton et al Adv Biosyst. 2020 Dec;4(12):e2000007. do

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Nov. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR-TRE-miRFP703_PGK-Puro

   Plasmid

   #216667

   Purpose

   Expresses tTa-inducible miRFP703, with puromycin resistance

   Depositor

   Leonardo Morsut

   Inserts

   miRFP703
   PuroR
   

   Use

   Lentiviral

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti puro HA-TUBA1B

   Plasmid

   #227587

   Purpose

   Lentiviral plasmid expressing human TUBA1B protein

   Depositor

   Melina Fan, Addgene Research Program

   Insert

   TUBA1B (TUBA1B Human)
   

   Use

   Lentiviral

   Tags

   HA

   Promoter

   CMV

   Available Since

   Nov. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. CPH3949_HIS-MBP-TEV-Tom1p-HECT-2850-3268

   Plasmid

   #227089

   Purpose

   Bacterial expression of HIS-MBP-TEV tagged Tom1p HECT domain (2850-3268)

   Depositor

   Chris Hill

   Insert

   tom1p-HECT (TOM1 Budding Yeast)
   

   Tags

   HIS-MBP-TEV_cleavage_site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. CPH3566_GST-TEV-Spt6p-tSH2-1223-1451

   Plasmid

   #227085

   Purpose

   Bacterial expression of GST-tagged Spt6 tSH2 domain (1223-1451)

   Depositor

   Chris Hill

   Insert

   spt6-tsh2 (SPT6 Budding Yeast)
   

   Tags

   GST-TEV_cleavage_site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRS415 GPDpro-RNQ1-CFP

   Plasmid

   #224887

   Purpose

   Low copy plasmid for constitutive yeast expression of RNQ1 tagged with CFP using copper. This is construct can be used as an IPOD marker (Kaganovich et al., 2008).

   Depositor

   Paul Herman

   Insert

   RNQ1 (RNQ1 Budding Yeast)
   

   Tags

   CFP

   Expression

   Yeast

   Promoter

   GPD

   Available Since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-phSyn-flex-SypHaloTag

   Plasmid

   #172280

   Purpose

   Cre-dependent HaloTag labeling of neuronal presynaptic terminals from the synapsin promoter.

   Depositor

   Danny Winder

   Insert

   synaptophysin-HaloTag
   

   Use

   AAV and Cre/Lox

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   rat synapsin

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. MDH2_EC

    Plasmid

    #224146

    Purpose

    IPTG induces expression of C. elegans protein MDH-2 with a N-terminal His tag.

    Depositor

    Katherine Walstrom

    Insert

    MDH-2 (mdh-2 Nematode)
    

    Tags

    His tag, Xpress tag, enterokinase cleaveage site

    Expression

    Bacterial

    Available Since

    Sept. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. AiP1999 - pAAV-AiE2255m-minBG-iCre(R297T)-BGHpA

    Plasmid

    #223843

    Purpose

    Enhancer-driven expression of the iCre(R297T) recombinase in thalamic PVT neurons

    Depositor

    Bosiljka Tasic

    Insert

    iCre(R297T)
    

    Use

    AAV

    Mutation

    Argenine in aa297 was replaced with Threonine

    Promoter

    minBG

    Available Since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFastBac HTB His-TEV-BN-rNLRP1-FLAG

    Plasmid

    #217323

    Purpose

    Purification of Brown Norway rat NLRP1 fusion with C-terminal FLAG tag from insect cells

    Depositor

    Yifan Wang

    Insert

    NLRP1
    

    Tags

    FLAG and His-TEV

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFastBac HTB His-TEV-LEW-rNLRP1-FLAG

    Plasmid

    #217320

    Purpose

    Purification of Lewis rat NLRP1 fusion with C-terminal FLAG tag from insect cells

    Depositor

    Yifan Wang

    Insert

    NLRP1
    

    Tags

    FLAG and His-TEV

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-GFAPp-PiGM-Iq(mCherry)

    Plasmid

    #221609

    Purpose

    A recombinant AAV2 plasmid encoding the PiGM-Iq system with CRY2PHR, CIBN and mCherry as expression marker. GFAP promoter for astrocyte-selective expression.

    Depositor

    John Lin

    Insert

    PiGM-Iq (D387A, mCherry)
    

    Use

    AAV

    Mutation

    RGS2 1-53 truncation, CRYPHR D387A

    Promoter

    GFAP promoter

    Available Since

    Aug. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. H2B-Electra2

    Plasmid

    #218929

    Purpose

    Electra2 fused to human H2B protein for nuclear localization

    Depositor

    Amro Hamdoun

    Insert

    H2B-Electra2 (H2B Entacmaea quadricolor, Human, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    Electra2 and H2B

    Expression

    Mammalian

    Available Since

    Aug. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. LvPolyUb

    Plasmid

    #218982

    Purpose

    L.variegatus polyubiquitin-C promoter

    Depositor

    Amro Hamdoun

    Insert

    L.variegatus polyubiquitinC promoter
    

    Use

    Sea urchin

    Promoter

    L.variegatus polyubiquitinC promoter

    Available Since

    Aug. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lmod1-BirA

    Plasmid

    #221509

    Purpose

    Flag-tagged biotinylation enzyme for co-expression of Lmod1

    Depositor

    Alessandro Ori

    Insert

    Lmod1 (Lmod1 Mouse)
    

    Tags

    BirA

    Expression

    Mammalian

    Available Since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. SYNJ1_BirA_Cterm

    Plasmid

    #221522

    Purpose

    Flag-tagged biotinylation enzyme for co-expression of Synj1

    Depositor

    Alessandro Ori

    Insert

    SYNJ1 (SYNJ1 Human)
    

    Tags

    BirA

    Expression

    Mammalian

    Available Since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. MUT_S1-spacer-H1

    Plasmid

    #218399

    Purpose

    3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron following a neutral spacer, and AG>GG mutation in final 3' splice site

    Depositor

    Oded Regev

    Insert

    Modified CHO DHFR minigene
    

    Expression

    Mammalian

    Promoter

    tetracycline-responsive promoter

    Available Since

    June 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. MUT_S1-H1,NR

    Plasmid

    #218395

    Purpose

    3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, both randomly mutated to avoid sequence repeats, and AG>GG mutation in final 3' splice site

    Depositor

    Oded Regev

    Insert

    Modified CHO DHFR minigene
    

    Expression

    Mammalian

    Promoter

    tetracycline-responsive promoter

    Available Since

    May 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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