We narrowed to 37,574 results for: tes

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pRetro-XT-PAK2-CFP(puro)-T402A

Plasmid

#81179

Purpose

Retroviral vector for expression of kinase inactive PAK2 with a CFP fusion

Depositor

Angeliki Malliri

Article

Whalley et al Nat Commun. 2015 Jun 16;6:7437. doi: 10.

Insert

p21 (RAC1) activated kinase 2 (PAK2 Human)

Use

Retroviral

Tags

CFP

Mutation

T402A

Promoter

P-tight TRE

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-hPPP3R1

Plasmid

#179136

Purpose

Expression vector of human protein phosphatase 3, regulatory subunit B, alpha isoform (PPP3R1), CAG promoter, rabbit globin poly(A) signal.

Depositor

Masahito Ikawa

Article

Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

Insert

protein phosphatase 3, regulatory subunit B, alpha isoform (PPP3R1 Human)

Expression

Mammalian

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pIS1 DICER1 long UTR

Plasmid

#21649

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

Dicer 1 long UTR (DICER1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pLV Reep3-V5

Plasmid

#175119

Purpose

Lentiviral expression of mouse Reep3-V5

Depositor

Larry Gerace

Article

Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

Insert

Reep3 (Reep3 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-YOD1

Plasmid

#85663

Purpose

Expression of human YOD1 with N-terminal mCherry tag

Depositor

Hemmo Meyer

Article

Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Insert

YOD1 (YOD1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pMRX-IBU-PFAS-FLAG

Plasmid

#168226

Purpose

Expresses human PFAS-3xFLAG fusion in mammalian cells

Depositor

Noboru Mizushima

Article

Mimura et al J Biol Chem. 2021 Jan-Jun;296:100780. do

Insert

PFAS (PFAS Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Available Since

April 26, 2021

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-MBP-HsNot4_AG

Plasmid

#148763

Purpose

Mammalian Expression of HsNot4

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4 (CNOT4 Human)

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLV Tmem9-V5

Plasmid

#175151

Purpose

Lentiviral expression of mouse Tmem9-V5

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Tmem9 (Tmem9 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pIRESneo2 mycBioID-Tollip

Plasmid

#178054

Purpose

myc-BioID fused to human Tollip cloned into the pIRESneo2 plasmid. For generation of stable expressing mammalian cells used for proximity dependent biotin labelling.

Depositor

David Tumbarello

Article

Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

Insert

Toll-Interacting Protein (TOLLIP Human)

Tags

mycBioID

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pAH18

Plasmid

#154397

Purpose

Derived from pGPI-SceI; Trimethoprim resistant; carries I-SceI gene; contains 475 bp from both upstream and downstream flanking regions between the FRT sites on pAHCTX1-rhadCas9

Depositor

Silvia Cardona

Article

Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

Insert

Fused upstream and downstream homologous regions around FRT sites when pAH-CTX1-rhadCas9 integrated into K56-2 genome

Use

Synthetic Biology

Expression

Bacterial

Promoter

NA

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pALS297 (ttTi5605-MCS loxP::Prps-27::NeoR::unc-54-3ʹutr::loxP)

Plasmid

#129538

Purpose

modified pCFJ151 for C. elegans mosSCI at ttTi5605 or oxTi354 in otherwise wild-type background; floxed NeoR selection

Depositor

Arneet Saltzman

Article

Aram et al MicroPubl Biol. 2019 Aug 27;2019. doi: 1

Type

Empty backbone

Use

Cre/Lox; Mos1-mediated single copy insertion (mos…

Expression

Worm

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pGAN200

Plasmid

#129203

Purpose

yTRAP sensor of [PSI+], detects aggregation of Sup35

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

Sup35NM (SUP35 Budding Yeast)

Use

Synthetic Biology

Expression

Yeast

Mutation

N terminal and middle domains

Promoter

SUP35

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV-SMYD2 F184A

Plasmid

#85659

Purpose

retroviral expression of human SMYD2 F184A

Depositor

Julien Sage

Article

Reynoird et al Genes Dev. 2016 Apr 1;30(7):772-85. doi:

Insert

SMYD2 (SMYD2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

F184A

Promoter

LTR

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pRGEB-VirD2-Cas9

Plasmid

#139803

Purpose

Expressing VirD-Cas9 fusion under ubiquitin promoter

Depositor

Magdy Mahfouz

Article

Ali et al Commun Biol. 2020 Jan 23;3(1):44. doi: 1

Insert

VirD2-Cas9

Tags

VirD2-Cas9

Expression

Plant

Promoter

Ubiquitin

Available Since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pFastBac-His6-Mps1-WT

Plasmid

#114063

Purpose

protein production

Depositor

Geert Kops

Article

Jelluma et al Cell. 2008 Jan 25;132(2):233-46. doi: 10

Insert

Mps1-WT (TTK Human)

Tags

His6

Expression

Insect

Mutation

none

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pU6-sgGAL4-4

Plasmid

#46916

Purpose

Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GAL4 UAS promoter (negative control)

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Inserts

sgGAL4-4
Puromycin resistance and mCherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

CMV and U6

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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GST-Bcr pLEF

Plasmid

#38160

Depositor

Nora Heisterkamp

Article

Mishra et al Oncogene. 2003 Nov 13;22(51):8255-62.

Insert

Bcr (BCR Human)

Tags

GST

Expression

Mammalian

Promoter

SV40

Available Since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only
pET51b-His-TEV-HOB_(HaloTag7_oligo_binder)

Plasmid

#167268

Purpose

Overexpression of the HaloTag7 variant HOB (halo-based oligonucleotide binder) in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

HOB

Tags

His x10

Expression

Bacterial

Promoter

T7

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pECE-M2-PAK2 S20A

Plasmid

#31681

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

p21-activated protein kinase 2 S20A (PAK2 Human)

Tags

M2

Expression

Mammalian

Mutation

changed Serine 20 to Alanine

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pET-15b-LanCL1

Plasmid

#154189

Purpose

To express LanCL1 in bacterial cells

Depositor

Wilfred van der Donk

Article

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

LanCL1

Tags

Hexahistidine

Expression

Bacterial

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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