We narrowed to 322 results for: cas9 f cas9 r

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UAS-FRT-GFP-FRT-u(M)Cas9

Plasmid

#127388

Purpose

Cas9 inducible by removal of a GFP flip-out cassette

Depositor

Michael Boutros

Article

Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

Insert

FRT-GFP-FRT-uORF(M)Cas9

Use

CRISPR

Expression

Insect

Available Since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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lentiCas9-EGFP

Plasmid

#63592

Purpose

Expresses human codon-optimized S. pyogenes Cas9 protein and EGFP from EFS promoter. 3rd generation lentiviral backbone.

Depositor

Phil Sharp, Feng Zhang

Article

Chen et al Cell. 2015 Mar 12;160(6):1246-60. doi: 1

Inserts

Cas9
EGFP

Use

CRISPR and Lentiviral

Tags

FLAG and P2A

Expression

Mammalian

Promoter

EFS-NS

Available Since

April 7, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-nEFCas9

Plasmid

#87115

Purpose

SpCas9 expression AAV backbone plasmid under control of nEF promoter

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

nEF-Cas9

Use

AAV and CRISPR

Tags

HA-NLS

Expression

Mammalian

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pJT256 Lenti pEF-dCas9-3XFLAG-N+F-T2A-tagBFP-P2A-Blast

Plasmid

#187332

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCas9-3XFLAG-N+F-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Promoter

pEF1a

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pJT257 Lenti pEF-dCas9-3XFLAG-Z+F-T2A-tagBFP-P2A-Blast

Plasmid

#187333

Purpose

dCas9 effector fusion for manipulating transcription

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

dCas9-3XFLAG-Z+F+N-T2A-tagBFP-P2A-Blast

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Promoter

pEF1a

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-SpyCas9

Plasmid

#113034

Purpose

AAV vector for expression of SpyCas9 in mammalian cells

Depositor

Dominik Niopek

Article

Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

Insert

SpyCas9

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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PB-TAC-OKMS-Cas9

Plasmid

#120353

Purpose

piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.

Depositor

Knut Woltjen

Article

Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

Insert

OKMS cassette

Use

CRISPR; Piggybac transposon

Expression

Mammalian

Mutation

Klf4 [10-483]

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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PX429 SpCas9 N863A nickase

Plasmid

#68706

Purpose

SpCas9 N863A nickase

Depositor

Feng Zhang

Article

Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

Insert

SpCas9 N863A nicking mutant

Use

CRISPR

Expression

Mammalian

Promoter

Cbh

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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PX544 SpCas9(del1098-1368)

Plasmid

#58889

Purpose

Truncated form of SpCas9 without C' domain

Depositor

Feng Zhang

Article

Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

Insert

hSpCas9

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Mutation

del amino acids 1098-1368

Available Since

Sept. 25, 2014

Availability

Academic Institutions and Nonprofits only

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PX543 SpCas9(del175-307)

Plasmid

#58888

Purpose

Truncated form of SpCas9 without REC2 domain

Depositor

Feng Zhang

Article

Nishimasu et al Cell. 2014 Feb 27;156(5):935-49. doi: 10

Insert

hSpCas9

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Mutation

del amino acids 175-307

Available Since

Sept. 25, 2014

Availability

Academic Institutions and Nonprofits only

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