Addgene

We narrowed to 2,384 results for: GCG

Showing: 81 - 100 of 2384 results

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pGL3-p107 1*

Plasmid

#32389

Depositor

Julien Sage

Article

Burkhart et al PLoS Genet. 2010 Jun 24;6(6):e1001003.

Insert

p107 promoter (Rbl1 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

1st E2F binding site mutation: GCG-->AAA

Promoter

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pNAS1B

Plasmid

#61968

Purpose

Duet vector for co-expression of GFP and mCherry from PBAD and PLtetO promoters, respectively; parent vector for split mCherry assembly vectors

Depositor

Lynne Regan

Article

Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

Inserts

mCherry
GFP

Use

Tags

Expression

Bacterial

Mutation

Serine 65 to Glycine (GGT), Serine 72 to Alanine …

Promoter

PBAD and PLtetO

Available since

Jan. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pSUPER-scramble

Plasmid

#118349

Purpose

This plasmid expresses scrabled shRNA for use as a control for shRNA HSFs plasmids.

Depositor

Lea Sistonen

Article

Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

Insert

scrambled shRNA

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-p107 1*2*

Plasmid

#32387

Depositor

Julien Sage

Article

Burkhart et al PLoS Genet. 2010 Jun 24;6(6):e1001003.

Insert

p107 promoter (Rbl1 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

1st and 2nd E2F binding sites mutated: GCG-->A…

Promoter

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pLKO.1-shCtnnd2.2-GFP

Plasmid

#209097

Purpose

GFP expressing shRNA targeting Ctnnd2 3' UTR

Depositor

Cagla Eroglu

Article

Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

Insert

shCtnnd2.2

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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MYMH 47

Plasmid

#138038

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-8AC-GFP

Use

Tags

Expression

Bacterial

Mutation

AAA GAG GCG AAA

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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MYMH 29

Plasmid

#138029

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-5AC-GFP

Use

Tags

Expression

Bacterial

Mutation

AAA GCG GAG AAA

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pBluescript GPX-1

Plasmid

#13038

Depositor

Peter Newburger

Article

Shen et al J Biol Chem. 1993 May 25. 268(15):11463-

Insert

GPX-1 (GPX1 Human)

Use

Tags

Expression

Bacterial

Mutation

Additional GCG upstream of codon 11. Codon 92 is …

Promoter

Available since

Nov. 7, 2006

Availability

Academic Institutions and Nonprofits only
pAFMW-Nbr[K276A]

Plasmid

#188597

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler (Nbr Fly)

Use

Tags

FLAG-Myc

Expression

Insect

Mutation

K276A [AAG=>GCG]

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr[K284A]

Plasmid

#188595

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler (Nbr Fly)

Use

Tags

FLAG-Myc

Expression

Insect

Mutation

K284A [AAG=> GCG]

Promoter

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pAFMW-Nbr[R272A]

Plasmid

#188594

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler (Nbr Fly)

Use

Tags

FLAG-Myc

Expression

Insect

Mutation

R272A [CGG=> GCG]

Promoter

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pSG5-HA-ERR3 S57,81,219A

Plasmid

#37850

Depositor

Rebecca Riggins

Article

Heckler et al FEBS J. 2014 Mar 29. doi: 10.1111/febs.1

Insert

Estrogen-related receptor gamma (Esrrg Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

changed Serines 57, 81, and 219 to Alanine (AGC t…

Promoter

SV40

Available since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only
pAFMW-Nbr[R280A,K284A]

Plasmid

#188598

Purpose

Flag-tagged Nibbler variants expression in Drosophila S2 cells

Depositor

Stefan Ameres

Article

Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

Nibbler (Nbr Fly)

Use

Tags

FLAG-Myc

Expression

Insect

Mutation

R280A [CGA=>GCA], K284A [AAG=> GCG]

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pJK01_Rho_minprox_DsREd

Plasmid

#173489

Purpose

PCR template for reporter gene with mouse Rhodopsin promoter with DsRed

Depositor

Barak Cohen

Article

White et al Proc Natl Acad Sci U S A. 2013 Jul 16;11

Insert

Mouse Rhodopsin promoter driving DsRed reporter gene

Use

Tags

Expression

Mammalian

Mutation

Promoter

Mouse Rhodopsin

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pgRNA-CGG

Plasmid

#108248

Purpose

Lentiviral vector to express sgRNA targeting "GGCGGCGGCGGCGGCGGCGG" locus by SpCas9

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2018 Feb 8. pii: S0092-8674(18)300

Insert

sgRNA targeting GGCGGCGGCGGCGGCGGCGG

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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pFlare9A-Clip2E9 Mutant

Plasmid

#209575

Purpose

minigene construct for Clip2 E9 alternative splicing, potential PTBP2 binding sites are deleted

Depositor

Sika Zheng

Article

Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

Insert

Clip2 (Clip2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

ACGCGTTTCTGAATTCTTCTAACTGCCCTCAAATGCACGGTGGCATGTG…

Promoter

CMV

Available since

March 7, 2024

Availability

Academic Institutions and Nonprofits only

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pSUPER-hHSF2 shRNA

Plasmid

#118346

Purpose

This plasmid expresses shRNA against hHSF2.

Depositor

Lea Sistonen

Article

Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

Insert

hHSF2 shRNA (HSF2 Human)

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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gCH134 (crCD55-4_crB2M-1_crB2M-3_crKIT-2_crKIT-3_crCD81-1)

Plasmid

#217342

Purpose

crRNA array targeting CD81, B2M, KIT, CD55

Depositor

Luke Gilbert

Article

Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

Inserts

crCD55-4 gRNA: actggtattgcggagccacgagg (CD55 Human)
crB2M-1 gRNA: atataagtggaggcgtcgcgctg (B2M Human)
crB2M-3 gRNA: aggaatgcccgccagcgcgacgc (B2M Human)
crKIT-2 gRNA: tctgcgttctgctcctactgctt (KIT Human)
crKIT-3 gRNA: agctctcgcccaagtgcagcgag (KIT Human)
crCD81-1 gRNA: ggcgcgacccccaggaaggtctc (CD81 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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FgH1tUTG

Plasmid

#70183

Purpose

Inducible expression of guide RNA with fluorescent GFP reporter

Depositor

Marco Herold

Article

Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

Insert

H1-Tet-sgrna cassette

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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Pmyo-3::hpo-30 gDNA (pBAB204)

Plasmid

#117385

Purpose

Allows for body-wall muscle expression of HPO-30

Depositor

Kavita Babu

Article

Sharma et al J Neurosci. 2018 Aug 8;38(32):7072-7087.

Insert

genomic DNA of hpo-30 (833 bp)

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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