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Showing: 81 - 100 of 352 results

  1. TRIPZ-CTID195-GSQ-UBnc-PURO

    Plasmid
    #208037
    Purpose
    Enables inducible expression of C-terminal Split-TurboID-fused UBnc, to perform Ubiquitin-ID; nc = non-cleavable mutation; selection with puromycin
    Depositor
    Rosa Barrio, James Sutherland
    Article
    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:
    Insert
    Ubnc (UBC Human)
    Use
    Lentiviral
    Tags
    Myc, C-terminal Split-TurboID
    Expression
    Mutation
    L73P Mutation near C-terminus suppresses cleavage…
    Promoter
    tetO/UBC
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208037-plasmid-map-sequence-id-410268

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  5. pB6-Id1-T2A-tdTomato-FNF-TK

    Plasmid
    #139281
    Purpose
    B6J Id1-T2A-tdTomato allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    Id1 (Id1 Mouse)
    Use
    Mouse Targeting
    Tags
    C-terminal fusion of T2A-tdTomato
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    139281-plasmid-map-sequence-id-317908

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  8. Lenti-EFS-NTID194-GSQ-PMLivaWT-P2A-BLAST

    Plasmid
    #208038
    Purpose
    Enables constitutive expression of N-terminal Split-TurboID-fused PML (isoform IVa; WT); to perform SUMO-ID; selection with blasticidin
    Depositor
    Rosa Barrio, James Sutherland
    Article
    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:
    Insert
    PML (isoform IVa) (PML Human)
    Use
    Lentiviral
    Tags
    FLAG, N-terminal Split-TurboID
    Expression
    Mutation
    Promoter
    EFS
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208038-plasmid-map-sequence-id-410203

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  12. Lenti-EFS-NTID194-GSQ-PMLiva3MAS-P2A-BLAST

    Plasmid
    #208039
    Purpose
    Enables constitutive expression of N-terminal Split-TurboID-fused PML (isoform IVa; 3MAS mutant; K>R sumoylation sites, mutated SIM); selection with blasticidin
    Depositor
    Rosa Barrio, James Sutherland
    Article
    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:
    Insert
    PML (isoform IVa; 3MAS) (PML Human)
    Use
    Lentiviral
    Tags
    FLAG, N-terminal Split-TurboID
    Expression
    Mutation
    3MAS mutant: K65R, K160R and K490R; mutated SIM
    Promoter
    EFS
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208039-plasmid-map-sequence-id-410207

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  19. pB6-Id1-IRES-loxP-NeoR-loxP-DTA-TK

    Plasmid
    #158616
    Purpose
    B6J Id1-LSL-DTA allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    Id1 (Id1 Mouse)
    Use
    Cre/Lox and Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Insertion of IRES-loxP-NeoR-loxP-DTA after the st…
    Promoter
    Available since
    May 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    158616-plasmid-map-sequence-id-333472

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  20. pB6-Id1-T2A-sfGFP-iCre-ERT2-FNF-TK

    Plasmid
    #139279
    Purpose
    B6J Id1-T2A-sfGFP-iCre-ERT2 allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    Id1 (Id1 Mouse)
    Use
    Cre/Lox and Mouse Targeting
    Tags
    C-terminal fusion of T2A-sfGFP-iCre-ERT2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    139279-plasmid-map-sequence-id-315358

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Showing: 81 - 100 of 352 results