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pBAMD1-4

Plasmid

#61565

Purpose

Standardized mini-Tn5 delivery plasmid, streptomycin/spectinomycin resistant

Depositor

Víctor de Lorenzo

Article

Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

Type

Empty backbone

Use

Synthetic Biology; Mini-tn5 vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pBAMD1-2

Plasmid

#61564

Purpose

Standardized mini-Tn5 delivery plasmid, kanamycin resistant

Depositor

Víctor de Lorenzo

Article

Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

Type

Empty backbone

Use

Synthetic Biology; Mini-tn5 vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pBAMD1-6

Plasmid

#61566

Purpose

Standardized mini-Tn5 delivery plasmid, gentamicin resistant

Depositor

Víctor de Lorenzo

Article

Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

Type

Empty backbone

Use

Synthetic Biology; Mini-tn5 vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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Hp MTAN

Plasmid

#69802

Purpose

Expression of H. pylori J99 methylthioadenosine nucleosidase

Depositor

Vern Schramm

Article

Wang et al Biochemistry. 2012 Sep 4;51(35):6892-4.

Insert

Methylthioadenosine nucleosidase

Use

Tags

Histidine tag

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only

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CUC1 pBluescript

Plasmid

#12064

Depositor

David Bartel

Article

Mallory et al Curr Biol. 2004 Jun 22. 14(12):1035-46.

Insert

CUC1 (CUC1 Mustard Weed)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 2, 2006

Availability

Academic Institutions and Nonprofits only
CUC1 pGreenII0129

Plasmid

#12066

Depositor

David Bartel

Article

Mallory et al Curr Biol. 2004 Jun 22. 14(12):1035-46.

Insert

CUC1 (CUC1 Mustard Weed)

Use

Plant transformation via agrobacterium

Tags

Expression

Mutation

Promoter

Available since

June 2, 2006

Availability

Academic Institutions and Nonprofits only
POC1486

Plasmid

#213811

Purpose

BpiI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BpiI-associated non-switchable methylase M2.HpyAII

Use

Tags

His6-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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POC1484

Plasmid

#213810

Purpose

BpiI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BpiI-associated non-switchable methylase M1.HpyAII

Use

Tags

His6-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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POC1483

Plasmid

#213809

Purpose

BpiI-associated non-switchable methylase

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BpiI-associated non-switchable methylase M1.HpyAII

Use

Tags

His6-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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pFd022.R2.RFP

Plasmid

#137971

Purpose

Expresses Prochlorococcus phage P-SSM2 phage Fd fused to mRFP

Depositor

Jonathan Silberg

Article

Campbell et al J Biol Chem. 2020 May 19. pii: RA120.013

Insert

Prochlorococcus phage P-SSM2 ferredoxin fused to monomeric RFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pFd029.R2.RFP

Plasmid

#137972

Purpose

Expresses Prochlorococcus marinus NATL1A Fd 1 fused to mRFP

Depositor

Jonathan Silberg

Article

Campbell et al J Biol Chem. 2020 May 19. pii: RA120.013

Insert

Prochlorococcus marinus NATL1A ferredoxin 1 fused to monomeric RFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pFd030.R2.RFP

Plasmid

#137973

Purpose

Expresses Prochlorococcus marinus NATL2A Fd 1 fused to mRFP

Depositor

Jonathan Silberg

Article

Campbell et al J Biol Chem. 2020 May 19. pii: RA120.013

Insert

Prochlorococcus marinus NATL2A ferredoxin 1 fused to monomeric RFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pFd031.RFP

Plasmid

#137974

Purpose

Expresses Prochlorococcus marinus MIT9211 Fd 1 fused to mRFP

Depositor

Jonathan Silberg

Article

Campbell et al J Biol Chem. 2020 May 19. pii: RA120.013

Insert

Prochlorococcus marinus MIT9211 ferredoxin 1 fused to monomeric RFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pCAMBIA1301-RmMYB108

Plasmid

#173180

Purpose

Overexpresses Rosa multiflora MYB108 in plants

Depositor

Daidi Che

Article

Dong et al Front Plant Sci. 2021 Jul 27;12:696919.

Insert

RmMYB108

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pBI121-GFP-RmMYB108

Plasmid

#173181

Purpose

Expresses Rosa multiflora MYB108 for subcellular localization in plants

Depositor

Daidi Che

Article

Dong et al Front Plant Sci. 2021 Jul 27;12:696919.

Insert

RmMYB108

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pGBKT7-RmMYB108

Plasmid

#173183

Purpose

Expresses Rosa multiflora MYB108 for transcriptional activation studies in yeast.

Depositor

Daidi Che

Article

Dong et al Front Plant Sci. 2021 Jul 27;12:696919.

Insert

RmMYB108

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMH-HT_CtCHAD

Plasmid

#127785

Purpose

Expresses CtCHAD in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

CtCHAD (CT0884 Chlorobium tepidum)

Use

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

Residues 208-522 (CHAD domain)

Promoter

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pGWT35S-AtOEP7(1-50aa)-mVenus

Plasmid

#194000

Purpose

Transient expression of AtOEP7-mVenus in plant cell (Chloroplast outer envelope membrane)

Depositor

Yutaka Kodama

Article

Ichikawa et al ACS Synth Biol. 2022 Oct 21;11(10):3190-

Insert

mVenus

Use

Tags

AtOEP7 1–50aa

Expression

Plant

Mutation

Promoter

CaMV 35S promoter

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pRCP

Plasmid

#195862

Purpose

Encodes chromogenic protein (CP). CP inducible with rhamnose.

Depositor

Gregory Phillips

Article

DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)

Insert

Chromogenic Protein

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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5mCUC1 pGreenII0129

Plasmid

#12067

Depositor

David Bartel

Article

Mallory et al Curr Biol. 2004 Jun 22. 14(12):1035-46.

Insert

CUC1 mutant (CUC1 Mustard Weed)

Use

Plant transformation via agrobacterium

Tags

Expression

Mutation

miR-resistant CUC1. Made by introducing five sile…

Promoter

Available since

June 2, 2006

Availability

Academic Institutions and Nonprofits only