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We narrowed to 415 results for: SaCas9

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Showing: 81 - 100 of 415 results

  1. JV201: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 TRE#1 gRNA

    Plasmid
    #131755
    Purpose
    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven SaCas9 TRE#1 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#1 gRNA expression
    Depositor
    Christopher Newgard
    Publication
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    TRE#1 SaCas9 gRNA
    Use
    Gateway Cloning and Synthetic Biology
    Promoter
    hU6
    Available since
    Oct. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    131755-plasmid-map-sequence-id-314448

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  2. LB101: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 TRE#2 gRNA

    Plasmid
    #131758
    Purpose
    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven SaCas9 TRE#2 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#2 gRNA expression
    Depositor
    Christopher Newgard
    Publication
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    TRE#2 SaCas9 gRNA
    Use
    Gateway Cloning and Synthetic Biology
    Promoter
    hU6
    Available since
    Oct. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    131758-plasmid-map-sequence-id-314395

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  3. pCAG-CFP-SaCas9-HF (without sgRNA)

    Plasmid
    #134470
    Purpose
    Human expression vector for SaCas9-HF variant.
    Depositor
    Zongli Zheng
    Publication
    Tan et al Proc Natl Acad Sci U S A. 2019 Sep 30. p
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Staphylococcus aureus Cas9 nuclease (SauCas9) with high genome-wide specificity
    Use
    CRISPR
    Tags
    SV40NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    R245A, N413A, N419A, R654A
    Promoter
    CAG
    Available since
    Nov. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    134470-plasmid-map-sequence-id-264583

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  5. pAID6

    Plasmid
    #136989
    Purpose
    Yeast integration plasmid for expression of dLbCpf1-VP, dSpCas9-RD1152, SaCas9, and Csy4
    Depositor
    Huimin Zhao
    Publication
    Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:
    Inserts
    dLbCpf1-VP
    dSpCas9-RD1152
    SaCas9
    Csy4
    Expression
    Yeast
    Promoter
    ENO2, TDH3, TEF1, and TPI1
    Available since
    May 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    136989-plasmid-map-sequence-id-269844

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  8. MSP1830

    Plasmid
    #70708
    Purpose
    Human expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    E782K, N968K and R1015H in SaCas9
    Promoter
    CAG
    Available since
    Nov. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    70708-plasmid-map-sequence-id-364684

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  12. MSP2292

    Plasmid
    #70707
    Purpose
    Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    E782K, N968K and R1015H in SaCas9
    Promoter
    T7
    Available since
    Dec. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    70707-plasmid-map-sequence-id-127770

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  13. pCSD_2xNLS-SpCas9MT3-NLS-3xHA-NLS-dSaCas9-2xNLS

    Plasmid
    #107318
    Purpose
    Expresses SpCas9 (R1335K) fused to dSaCas9 in mammalian cells
    Depositor
    Scot Wolfe
    Publication
    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-SaCas9 fusion
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    SpCas9 (R1335K) fused to nuclease dead SaCas9 (D1…
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107318-plasmid-map-sequence-id-204674

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  14. pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-dSaCas9-2xNLS

    Plasmid
    #107319
    Purpose
    Expresses SpCas9 fused to dSaCas9 in mammalian cells
    Depositor
    Scot Wolfe
    Publication
    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-SaCas9 fusion
    Use
    CRISPR
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    SpCas9 is fused to nuclease dead SaCas9 (D10A, N5…
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107319-plasmid-map-sequence-id-204682

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  20. AAV-CMV-TetRC-2A-Tomato-bGHpA;U6_BsaI-sgRNA

    Plasmid
    #177365
    Purpose
    AAV expression of Chimeric guide for SaCas9 and reverse tetracycline-controlled transactivator for doxycycline controlled expression
    Depositor
    Naoki Yamamoto
    Publication
    Yamamoto et al Research Square [Preprint] 2021. doi: 10
    Inserts
    tdTomato
    Chimeric guide for SaCas9
    Use
    AAV
    Tags
    P2A and a tetracycline-controlled transactivator …
    Expression
    Mammalian
    Promoter
    CMV and U6 promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    177365-plasmid-map-sequence-id-353664

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Showing: 81 - 100 of 415 results