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We narrowed to 190 results for: amh

Showing: 81 - 100 of 190 results
  1. G protein alpha-s-GFP

    Plasmid
    #66083
    Purpose
    G protein alpha-s internally tagged with EGFP and EE epitope
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    G-alpha-s-EE-EGFP (Gnas Rat)
    Tags
    EGFP flanked by SGGGS on each side was inserted i…
    Expression
    Mammalian
    Mutation
    BamHI sites in the alpha-s cDNA were removed and …
    Promoter
    CMV
    Available since
    Aug. 12, 2015
    Availability
    Academic Institutions and Nonprofits only
  2. Cas9/pTREX-n

    Plasmid
    #68708
    Purpose
    Expresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone. This vector is used for cloning a specific sgRNA by BamHI, to be co-expressed with Cas9 for genome editing in Trypanosoma cruzi.
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene) .
    Use
    CRISPR
    Tags
    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 …
    Mutation
    C4239T mutation that eliminates a BamHI restricti…
    Available since
    Sept. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  3. pPK605

    Plasmid
    #38148
    Depositor
    Inserts
    unc-119 rescuing fragment (unc-119 promoter, unc-119 and unc-119 3'UTR) (unc-119 Nematode)
    pie-1 promoter - unique MluI/BamHI cloning site - pie-1 3'UTR
    Expression
    Worm
    Promoter
    pie-1 promoter and unc-119
    Available since
    Oct. 25, 2012
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  4. G protein alpha-q-GFP

    Plasmid
    #66080
    Purpose
    This G protein alpha-q construct contains internal insertions of GFP and the EE epitope
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    G protein alpha-q-EE-YFP (Gnaq Mouse)
    Tags
    GFP was inserted internally into the alpha-q sequ…
    Expression
    Mammalian
    Mutation
    BamHI and SacI sites in alpha-q were removed with…
    Promoter
    CMV
    Available since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  5. pBABE-MCS(extended)-Puro (empty vector)

    Plasmid
    #172603
    Purpose
    Empty retroviral vector for the constitutive, near-physiological expression of a gene of interest and a puromycin resistance marker in mammalian cells.
    Depositor
    Type
    Empty backbone
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    The multiple cloning site (MCS) has been edited/e…
    Available since
    Aug. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
  6. Golden GATEway Cloning Kit

    Plasmid kit
    #1000000054
    Purpose
    A collection that combines Golden Gate with Multisite Gateway cloning for assembly of complex fusion proteins or transgenesis constructs.
    Depositor
    Application
    Cloning and Synthetic Biology
    Vector type
    Bacterial Expression, Eukaryotic Expression, Mammalian Expression, Zebrafish Expression
    Cloning type
    Golden Gate
    Available since
    Sept. 10, 2014
    Availability
    Academic Institutions and Nonprofits only
  7. Target Accelerator Pan-Cancer Mutant Collection

    Plasmid kit
    #1000000103
    Purpose
    Gateway entry vectors containing wildtype and mutant alleles found across cancer types for "pan-cancer" analyses.
    Depositor
    Application
    Gene Expression and Labeling
    Vector type
    Mammalian Expression
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
  8. G protein alpha-s-HaloTag

    Plasmid
    #66993
    Purpose
    G protein alpha-s internally tagged with HaloTag and EE epitope
    Depositor
    Insert
    G-alpha-s-EE-HaloTag (Gnas Rat)
    Tags
    HaloTag flanked by SGGGGSGGGGS on each side was i…
    Expression
    Mammalian
    Mutation
    BamHI sites in the alpha-s cDNA were removed and …
    Promoter
    CMV
    Available since
    Aug. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
  9. G protein alpha-o-YFP

    Plasmid
    #55776
    Purpose
    This G protein alpha-o construct contains internal insertions of YFP and the EE epitope
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    G protein alpha-o internally tagged with EYFP and EE epitope (Gnao1 Rat)
    Tags
    EE epitope was introduced internally with D167E a…
    Expression
    Mammalian
    Mutation
    A BglII site was removed from the 5' untrans…
    Promoter
    CMV
    Available since
    Aug. 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  10. G protein alpha-s-CFP

    Plasmid
    #55793
    Purpose
    G protein alpha-s internally tagged with ECFP and EE epitope
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    G-alpha-s-EE-ECFP (Gnas Rat)
    Tags
    ECFP flanked by SGGGS on each side was inserted i…
    Expression
    Mammalian
    Mutation
    His was substituted for Asn164 in ECFP (Clontech)…
    Promoter
    CMV
    Available since
    Aug. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  11. G protein alpha-s-mCherry

    Plasmid
    #66968
    Purpose
    G protein alpha-s internally tagged with mCherry and EE epitope
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    G-alpha-s-EE-mCherry (Gnas Discosoma sp., Rat)
    Tags
    internal EE epitope (residues 189-194 in alpha- s…
    Expression
    Mammalian
    Mutation
    BamHI sites in the alpha-s cDNA were removed and …
    Promoter
    CMV
    Available since
    Aug. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
  12. YFP(159-238)-gamma-2 in pcDNAI/Amp

    Plasmid
    #54472
    Purpose
    A carboxyl-terminal YFP fragment was fused to Ggamma-2. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    YFP(159-238)-gamma -2 (GNG2 Human, Aequorea victoria)
    Tags
    YFP(159-238) was fused to the amino terminus of G…
    Expression
    Mammalian
    Mutation
    Ggamma-2 was amplified by PCR, which added a BamH…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
  13. YFP(1-158)-beta-1 in pcDNAI/Amp

    Plasmid
    #54468
    Purpose
    An amino-terminal YFP fragment was fused to Gbeta-1. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    YFP (1-158)/beta-1 (GNB1 Human, Aequorea victoria)
    Tags
    YFP(1-158) was fused to the amino terminus of Gbe…
    Expression
    Mammalian
    Mutation
    GBeta-1 was amplified via PCR, which removed an i…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
  14. Cer(1-158)-RGS7

    Plasmid
    #55760
    Purpose
    An amino-terminal mCerulean fragment was fused to RGS7. When co-expressed with a carboxyl terminal fragment of CFP fused to Gbeta-5, a fluorescent signal is produced.
    Depositor
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCer(1-158)-RGS7 (RGS7 Human, Aequorea victoria)
    Tags
    Cer(1-158) was fused to the amino terminus of RGS…
    Expression
    Mammalian
    Mutation
    RGS7 was amplified via PCR, which added an N-term…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
  15. pETDuet-1 TIM9,10

    Plasmid
    #170280
    Purpose
    Expresses both yeast Tim9 and yeast Tim10 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production
    Depositor
    Insert
    mitochondrial Tim9 chaperone subunit and Tim10 (with N-ter His tag) (TIM9, TIM10 Budding Yeast)
    Expression
    Bacterial
    Promoter
    Tim9: via NdeI/XhoI into the MCS2; Tim10: via Bam…
    Available since
    June 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
Showing: 81 - 100 of 190 results