We narrowed to 13,054 results for: sequence

1. pQE-9-RTXMCR-TLY-X2N-C

   Plasmid

   #225974

   Purpose

   Repeats-In-Toxin (RTX) protein consensus sequence GGAGNDTLY repeated nine times with C-terminal Capping Domain

   Depositor

   Danielle Mai

   Article

   Chang et al Polym Chem. 2024. doi: https://doi.org/1

   Insert

   RTXMCR-TLY-X2N-C
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pQE-9-RTXMCR-TLY-X2D-C

   Plasmid

   #225975

   Purpose

   Repeats-In-Toxin (RTX) protein consensus sequence GGAGDDTLY repeated nine times with C-terminal Capping Domain

   Depositor

   Danielle Mai

   Article

   Chang et al Polym Chem. 2024. doi: https://doi.org/1

   Insert

   RTXMCR-TLY-X2D-C
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pQE-9-RTXMCR-TLY-X2E-C

   Plasmid

   #225976

   Purpose

   Repeats-In-Toxin (RTX) protein consensus sequence GGAGEDTLY repeated nine times with C-terminal Capping Domain

   Depositor

   Danielle Mai

   Article

   Chang et al Polym Chem. 2024. doi: https://doi.org/1

   Insert

   RTXMCR-TLY-X2E-C
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. HS_NAM-B1

   Plasmid

   #154064

   Purpose

   Level M, P1 Golden Gate vector. OsAct::Hyg-nosT/HvHSP17::Cre-hspT/ZmUbi::loxP-GUS-loxP-NAM-B1-nosT

   Depositor

   Cristobal Uauy

   Article

   Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

   Inserts

   Hygromycin phosphotransferase
   Cre recombinase
   LoxP-flanked GUS and TtNAM-B1
   

   Use

   Cre/Lox and Synthetic Biology; Golden gate

   Tags

   Expression

   Bacterial and Plant

   Mutation

   The TtNAM-B1 gene sequence was domesticated to re…

   Promoter

   HvHSP17, OsAct, and ZmUbi

   Available since

   Sept. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. C40m

   Plasmid

   #120973

   Purpose

   MoClo golden gate assembly CD part for blank part (random 50bp sequence) - Please see Supplemental Documents for annotated Genbank file

   Depositor

   Richard Murray

   Insert

   Blank (Random 50bp sequence)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. eft-3p:act-5(ptc):tbb2 3' UTR

   Plasmid

   #202331

   Purpose

   The ubiquitous eft-3 promoter drives overexpression of the mutant act-5(ptc) gene including introns.

   Depositor

   Didier Stainier

   Article

   Welker et al PLoS Genet. 2023 Jun 29;19(6):e1010806.

   Inserts

   eft-3 promoter
   act-5(ptc)
   tbb-2 3' UTR
   

   Use

   Tags

   base 238 C -> A mutation causing a PTC at codo…

   Expression

   Worm

   Mutation

   Promoter

   This is a promoter sequence for C. elegans eft-3,…

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPK-352

   Plasmid

   #157922

   Purpose

   pcDNA-CMV-PCYA-IRES-HO1-P2A-FD-P2A-FNR

   Depositor

   Phillip Kyriakakis

   Article

   Kyriakakis et al J Vis Exp. 2021 Jan 12;(167). doi: 10.37

   Inserts

   tPcyA
   IRES
   HO1
   FD
   FNR
   

   Use

   Synthetic Biology; Optogenetics

   Tags

   Mitochondrial Targeting Sequence, Mitochondrial T…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p210 pCMV-CREM

   Plasmid

   #8395

   Purpose

   Cre with chimeric/β-globin intron within the Cre coding sequence

   Depositor

   Jeffrey Green

   Article

   Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

   Insert

   CREM
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   modified Cre

   Promoter

   CMV

   Available since

   July 18, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. FLAG-hLRP6-opt(20-1613)-9xHis_MESD_pFastBacDual

   Plasmid

   #216377

   Purpose

   Baculovirus transfer vector to co-express FLAG-LRP6-His (human sequence, codon-optimized for insect cell expression) and MESD chaperone (human sequence)

   Depositor

   William Weis

   Insert

   LDL receptor related protein 6 (LRP6 Human)
   

   Use

   Baculovirus

   Tags

   9xHis and HA signal sequence-FLAG-3C cleavage sit…

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin (LRP6)/p10 (MESD)

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLSLGUS

    Plasmid

    #51517

    Purpose

    pLSL with GUS coding sequence followed by 3 kb of filler sequence Rep is not included on this plasmid

    Depositor

    Daniel Voytas

    Article

    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

    Inserts

    GUS
    3kb filler sequence
    

    Use

    Plant t-dna plasmid

    Tags

    NLS and flag

    Expression

    Mutation

    Promoter

    virion-sense LIR and 2x35S

    Available since

    May 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pX330_HR_Prnp_3

    Plasmid

    #78621

    Purpose

    pX330 vector encoding SpCas9 and a chimeric guide RNA targeting Prnp coding sequence

    Depositor

    Walker Jackson

    Article

    Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

    Insert

    pX330_HR_Prnp_3
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. psiCHECK-EGFP

    Plasmid

    #196606

    Purpose

    Dual-Luciferase Reporter vector carrying an EGFP direct sequence

    Depositor

    Franco Lucchini

    Article

    Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

    Insert

    GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLentiGuide-BlastR

    Plasmid

    #196970

    Purpose

    Lentiviral acceptor vector for pegRNAs with blasticidin resistance

    Depositor

    Leopold Parts

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. C79m

    Plasmid

    #120991

    Purpose

    MoClo golden gate assembly CD part for ccdA (Antitoxin to ccdB/C39m. Sequence taken from pMAR7 plasmid sequence). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    ccdA antitoxin
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCCG_nit9fl

    Plasmid

    #228409

    Purpose

    Expression of NIT-9 in N. crassa at the his-3 locus

    Depositor

    Robert Haensch

    Insert

    NIT-9
    

    Use

    Protein expression in n. crassa

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mCherry_C1_miR21

    Plasmid

    #65970

    Purpose

    Expressed mCherry under the control of miR-21 (miR-21 complementary sequence in 3' UTR)

    Depositor

    Thomas Tuschl

    Insert

    miR-21 complementary sequence
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV_Efla_FLEX_APEX2-eGFPf

    Plasmid

    #171939

    Purpose

    Expresses a membrane targeted (farnesylation) APEX2-eGFP fusion protein

    Depositor

    David Berson

    Insert

    APEX2-eGFPf
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV_Ef1a_FLEX_mAPEX2_P2A_mRuby2

    Plasmid

    #171938

    Purpose

    Expresses membrane targeted (palmitoylation) APEX2 and mRuby2 as separate proteins

    Depositor

    David Berson

    Insert

    Apex2, mRuby2
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV_hsyn_FLEX_tau-APEX2

    Plasmid

    #171937

    Purpose

    Expresses APEX2 linked to tau for labeling microtubules

    Depositor

    David Berson

    Insert

    tau-APEX2
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hsyn

    Available since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCCG_nit9_without_linker

    Plasmid

    #228411

    Purpose

    Expression of NIT-9 without linkage region in N. crassa at the his-3 locus

    Depositor

    Robert Haensch

    Insert

    NIT-9
    

    Use

    Protein expression in n. crassa

    Tags

    Expression

    Mutation

    deletion of the linkage region

    Promoter

    Available since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge