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We narrowed to 10,323 results for: Coli

Showing: 1961 - 2000 of 10323 results
  1. pLN43 - pBait-1xMS2-7GC[chiP-5']-TtrpA

    Plasmid
    #222404
    Purpose
    arabinose-induced synthesis of a hybrid RNA containing a 5' MS2 hairpin, E. coli chiP 5'UTR insert between XmaI and HindIII sites, surrounded by a 7bp GC clamp, and followed by a 3' trpA terminator.
    Depositor
    Insert
    5’UTR of chiP (-98 to +12, relative to AUG) (chiP E. coli)
    Tags
    1xMS2 hairpin, 7GC clamp, TtrpA terminator
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    Jan. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
  2. PTet-F30-Pepper aptamer-UTR1-mTurq2

    Plasmid
    #227645
    Purpose
    pTet-driven expression of F30-scaffolded Pepper aptamer and mTurquoise2 for quantifying cell-free RNA and protein synthesis. The mTurq2 has a GGGGS linker followed by a His6x tag.
    Depositor
    Insert
    F30-scaffolded Pepper aptamer and mTurquoise2 (codon optimized for E. coli B using IDT Codon Optimizer)
    Use
    Synthetic Biology
    Available Since
    Nov. 14, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. pCDF-NtXNtS1HR1AtR2NtB2

    Plasmid
    #160890
    Purpose
    Expression of N. tabacum Rubisco small subunit (rbcS-SH1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.
    Depositor
    Insert
    PT7-Nt-rbcS-S1H
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    rbcS-SH1: T7, chaperones: T13
    Available Since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
  4. pVR2-m2

    Plasmid
    #84721
    Purpose
    pVR2 but with a G to U mutation in the riboswitch aptamer
    Depositor
    Inserts
    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    Expression
    Bacterial
    Mutation
    G to U mutation at position 16 of annotated "…
    Available Since
    March 4, 2019
    Availability
    Academic Institutions and Nonprofits only
  5. pVR2-m3

    Plasmid
    #84722
    Purpose
    pVR2 but with a C to G mutation in the riboswitch aptamer
    Depositor
    Inserts
    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    Expression
    Bacterial
    Mutation
    C to G mutation at position 22 of annotated "…
    Available Since
    March 4, 2019
    Availability
    Academic Institutions and Nonprofits only
  6. pVR2-m1

    Plasmid
    #84720
    Purpose
    pVR2 but with a C to U mutation in the riboswitch aptamer
    Depositor
    Inserts
    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    Expression
    Bacterial
    Mutation
    C to U mutation at position 13 of annotated "…
    Available Since
    March 4, 2019
    Availability
    Academic Institutions and Nonprofits only
  7. pHL 1410

    Plasmid
    #53011
    Purpose
    pLtetO-1:DsrA, pCon:ompC::T7noRBS(900bps), pLlacO-1:rpoS::gfp
    Depositor
    Inserts
    DsrA sRNA
    ompC
    rpoS
    Use
    Synthetic Biology
    Tags
    T7noRBS (900bp) and gfp
    Expression
    Bacterial
    Mutation
    contains −150 to +30 bp relative to start codon a…
    Promoter
    pCon, pLlacO-1, and pLtetO-1
    Available Since
    Jan. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
  8. pHL 762

    Plasmid
    #52990
    Purpose
    pLtetO-1:MicC, pLtetO-1:sodB::mCherry, pLlacO-1:ompC::gfp
    Depositor
    Inserts
    MicC sRNA
    sodB
    ompC
    Use
    Synthetic Biology
    Tags
    gfp and mCherry
    Expression
    Bacterial
    Mutation
    −56 to +141 bp and −81 to +36 bp
    Promoter
    pLlacO-1 and pLtetO-1
    Available Since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only
  9. pHL1529

    Plasmid
    #53027
    Purpose
    PLlacO-1:glgC::gfp, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st2):csrA
    Depositor
    Inserts
    GFP
    tetR
    csrA
    Use
    Synthetic Biology
    Tags
    RBS(st2), RBS(st3), and glgC leader contains -61 …
    Expression
    Bacterial
    Promoter
    pConNoHind and pLlac0-1
    Available Since
    May 13, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 1961 - 2000 of 10323 results