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pEMF038

Plasmid

#157635

Purpose

Empty vector

Depositor

Jonathan Silberg

Article

Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

Type

Empty backbone

Expression

Bacterial

Available Since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pReplace_donor_mcherry

Plasmid

#149345

Purpose

Donor vector for the replacement of reporter BFP by mcherry

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

mcherry

Expression

Mammalian

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pFD-ePDZ-B1

Plasmid

#137113

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

ePDZ-B1

Use

Synthetic Biology

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pFD-FN3

Plasmid

#137112

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

FN3

Use

Synthetic Biology

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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spoT-

Plasmid

#100913

Purpose

deletes synpcc7942_1377 (spoT)

Depositor

Erin O'Shea

Article

Puszynska et al Cell Rep. 2017 Dec 12;21(11):3155-3165.

Insert

Kanamycin casette (in place of spoT gene) flanked by S. elongatus sequence

Use

Cloning vector

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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relA+_NS1

Plasmid

#100916

Purpose

expresses E.coli relA+ from the NS1 site in the S. elongatus PCC7942 genome

Depositor

Erin O'Shea

Article

Puszynska et al Cell Rep. 2017 Dec 12;21(11):3155-3165.

Insert

RelA fragment from E.coli

Use

Cyanobacteria cloning vector

Tags

FLAG

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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KFP08

Plasmid

#119798

Purpose

SigF2 epitope tagged construct for integration at neutral site 2.2

Depositor

Erin O'Shea

Article

Fleming et al Cell Rep. 2018 Dec 11;25(11):2937-2945.e

Insert

Synpcc7942_1784

Expression

Bacterial

Available Since

Dec. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pBsaGSPdR

Plasmid

#108205

Purpose

PdR

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

Putidaredoxin reductase

Expression

Bacterial

Promoter

lac promoter

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pHPro54G

Plasmid

#108204

Purpose

4th Pro5

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

Pro-Pro-Pro-Pro-Pro

Expression

Bacterial

Promoter

lac promoter

Available Since

April 15, 2018

Availability

Academic Institutions and Nonprofits only

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pMal672H

Plasmid

#25817

Depositor

Cynthia Wolberger

Article

Feeser et al J Mol Biol. 2008 Jul 11. 380(3):520-31.

Insert

Rap1(672-827) (RAP1 Budding Yeast)

Tags

His6 and MBP

Expression

Bacterial

Available Since

July 26, 2010

Availability

Academic Institutions and Nonprofits only
pET21a.AQ_1575

Plasmid

#11416

Depositor

Sung-Hou Kim

Article

Shin et al Proc Natl Acad Sci U S A. 2002 Jun 11. 9

Insert

hypothetical (aq_1575 A. aeolicus)

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut A

Plasmid

#11314

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation A (see paper) on inner 2 binding sites.

Available Since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut B

Plasmid

#11315

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation B (see paper) on inner 2 binding sites.

Available Since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut C

Plasmid

#11316

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation C (see paper) on inner 2 binding sites.

Available Since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut D

Plasmid

#11317

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation D (see paper) on inner 2 binding sites.

Available Since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut E

Plasmid

#11318

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation E (see paper) on inner 2 binding sites.

Available Since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut F

Plasmid

#11319

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation F (see paper) on inner 2 binding sites.

Available Since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut H

Plasmid

#11321

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation H (see paper) on inner 2 binding sites.

Available Since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut GFP

Plasmid

#11323

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 bulged binding sites for CXCR4 siRNA antisense, flanking 2 binding sites for GFP

Use

Luciferase

Tags

Rr-luc

Available Since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pRB327

Plasmid

#8668

Depositor

David Botstein

Article

Schatz et al Mol Cell Biol 1986 Nov;6(11):3722-33.

Insert

TUB1 (TUB1 Budding Yeast)

Expression

Yeast

Available Since

June 30, 2005

Availability

Academic Institutions and Nonprofits only